食品与发酵工业 >

2026 , Vol. 52 >Issue 17: 356 - 364

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.045206

分析与检测

基于超高效液相色谱-高分辨质谱的茶叶非挥发性成分分析与品质评价

  • 黎露露 ,
  • 尹艳艳 ,
  • 王夜梅 ,
  • 张梦瑞 ,
  • 许文静 ,
  • 路虎 ,
  • 范奇高
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  • 1(贵州习酒股份有限公司,贵州 遵义,564622)
    2(酱香型白酒生物制造技术贵州省院士创新团队工作站,贵州 遵义,564622)
第一作者:硕士,工程师(范奇高工程师为通信作者,E-mail:1354326886@qq.com)

收稿日期: 2025-11-18

  修回日期: 2026-01-25

  网络出版日期: 2026-09-21

基金资助

遵义市科技创新团队建设项目(遵KCTD074)

收起

Analysis and quality evaluation of non-volatile components in tea based on ultra-high performance liquid chromatography-high resolution mass spectrometry

  • LI Lulu ,
  • YIN Yanyan ,
  • WANG Yemei ,
  • ZHANG Mengrui ,
  • XU Wenjing ,
  • LU Hu ,
  • FAN Qigao
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  • 1(Guizhou Xijiu Co.Ltd., Zunyi 564622, China)
    2(Guizhou Academician Innovation Team Workstation of Jiangxiangxing Baijiu Bio-Manufacturing Technology, Zunyi 564622, China)

Received date: 2025-11-18

  Revised date: 2026-01-25

  Online published: 2026-09-21

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摘要

为实现茶叶中儿茶素、氨基酸及生物碱等多种非挥发性成分的同步分析,该研究建立了一种基于超高效液相色谱-高分辨质谱的定性定量分析方法。以0.1%甲酸-50%甲醇(体积分数)作为提取溶剂,通过超声辅助同步提取29种目标化合物。通过系统优化,采用Hypersil GOLD色谱柱(150 mm×2.1 mm,1.9 μm),以0.1%甲酸水(体积分数)和甲醇为流动相进行梯度洗脱,在正负离子切换模式下实现儿茶素、生物碱和氨基酸成分的同步分离。方法学验证表明,29种目标物在其线性范围内决定系数R2＞0.988 7,加标回收率为80.77%~117.23%,相对标准偏差为0.59%~9.81%,表明方法准确度高、稳定性好。将该方法应用于实际样品分析,基于29种成分进行主成分分析,提取到5个主成分,累计方差贡献率为97.39%,并构建茶叶品质综合评价模型。该方法操作简便、灵敏度高,适用于茶叶多组分同步检测与品质综合评价,为茶叶质量控制和标准化评价提供了参考。

关键词： 茶叶; 非挥发性成分; 超高效液相色谱-高分辨质谱; 同时测定; 品质评价

本文引用格式

黎露露 , 尹艳艳 , 王夜梅 , 张梦瑞 , 许文静 , 路虎 , 范奇高 . 基于超高效液相色谱-高分辨质谱的茶叶非挥发性成分分析与品质评价[J]. 食品与发酵工业, 2026 , 52(17) : 356 -364 . DOI: 10.13995/j.cnki.11-1802/ts.045206

Abstract

This study developed a method based on ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) to achieve simultaneous analysis of multiple non-volatile components in tea.A solution of 0.1% formic acid-50% methanol (volume fraction) was used as the extraction solvent for the ultrasonic-assisted simultaneous extraction of 29 target compounds, including catechins, amino acids, and alkaloids.After systematic optimization, a Hypersil GOLD column (150 mm×2.1 mm, 1.9 μm) was used, with a gradient elution of 0.1% formic acid water (volume fraction) and methanol as the mobile phase.The simultaneous separation and identification of catechins, alkaloids, and amino acids were achieved under positive and negative ion switching modes.Method validation showed good linearity (R2>0.988 7) for all analytes within their respective concentration ranges.The average recoveries at three spiked levels ranged from 80.77% to 117.23%, with relative standard deviations between 0.59% and 9.81%.These results indicated that the method provided high accuracy, repeatability, and stability.The established method was successfully applied to real tea samples.Based on the contents of the 29 components, principal component analysis was performed, extracting five principal components with a cumulative variance contribution rate of 97.39%.A comprehensive quality evaluation model for tea was subsequently constructed.This method is simple, sensitive, and suitable for simultaneous determination and quality assessment of multiple components in tea, providing a valuable reference for tea quality control and standardized evaluation.

Key words： tea; non-volatile compounds; ultra-high performance liquid chromatography-high resolution mass spectrometry; simultaneous determination; quality evaluation

参考文献

[1] 曾鸿哲, 文帅, 方雯雯, 等. 茶叶活性成分健康功能研究新进展[J]. 中国茶叶, 2025, 47(2):1-13.
Zeng H Z, Wen S, Fang W W, et al. Research progress on the health functions of tea active ingredients[J]. China Tea, 2025, 47(2):1-13.
[2] 刘静, 李丽, 孙玉香, 等. 茶叶中主要化学成分的质谱分析[J]. 福建茶叶, 2020, 42(6):8-9.
Liu J, Li L, Sun Y X, et al. Mass spectrometry analysis of main chemical components in tea[J]. Tea in Fujian, 2020, 42(6):8-9.
[3] 彭凯雄, 郑钰涵, 徐天泽, 等. 茶叶活性成分的功能及其健康食品研究进展[J]. 粮食与油脂, 2023, 36(3):17-20.
Peng K X, Zheng Y H, Xu T Z, et al. Research progress on the function and healthy food of tea active components[J]. Cereals & Oils, 2023, 36(3):17-20.
[4] Luo Q X, Luo L B, Zhao J M, et al. Biological potential and mechanisms of tea’s bioactive compounds: An Updated review[J]. Journal of Advanced Research, 2024, 65:345-363.
[5] Winiarska-Mieczan A, Tomaszewska E, Jachimowicz K. Antioxidant, anti-inflammatory, and immunomodulatory properties of tea-the positive impact of tea consumption on patients with autoimmune diabetes[J]. Nutrients, 2021, 13(11):3972.
[6] Wu Z Y, Sun L L, Chen R H, et al. Chinese tea alleviates CCl4-induced liver injury through the NF-κB or Nrf2 signaling pathway in C57BL-6J mice[J]. Nutrients, 2022, 14(5):972.
[7] 孙雨宁, 刘倩芬, 刘丹, 等. 茶叶缓解结肠炎及其机制研究进展[J]. 食品科学, 2025, 46(18):425-439.
Sun Y N, Liu Q F, Liu D, et al. Progress in understanding the role and mechanism of action of tea in alleviating colitis[J]. Food Science, 2025, 46(18):425-439.
[8] Xu X Y, Zhao C N, Cao S Y, et al. Effects and mechanisms of tea for the prevention and management of cancers: An updated review[J]. Critical Reviews in Food Science and Nutrition, 2020, 60(10):1693-1705.
[9] 赵东贤. 黄山毛峰茶多酚提取工艺优化及体外抗菌活性研究[J]. 吉林农业科技学院学报, 2024, 33(6):1-4; 9.
Zhao D X. Optimization of extraction process and in vitro antimicrobial activity of Huangshan Maofeng tea polyphenols[J]. Journal of Jilin Agricultural Science and Technology College, 2024, 33(6):1-4; 9.
[10] 黄逢阳, 李浩, 冯玮, 等. 黑茶的抗肥胖功效及其作用机制研究进展[J]. 食品安全质量检测学报, 2024, 15(4):242-249.
Huang F Y, Li H, Feng W, et al. Research progress on the anti-obesity efficacy of dark tea and its mechanism of action[J]. Journal of Food Safety and Quality, 2024, 15(4):242-249.
[11] 刘昕, 吴建铭. 高效液相色谱法同时检测茶叶提取物中9种儿茶素类和3种生物碱类化合物[J]. 香料香精化妆品, 2024(1):114-118.
Liu X, Wu J M. Simultaneous determination of nine catechins and three alkaloids in tea extracts by HPLC[J]. Flavour Fragrance Cosmetics, 2024(1):114-118.
[12] 李倩, 邵金良, 尹本林, 等. 不同加工方式的云南大叶种茶生物活性成分差异分析[J]. 食品工业科技, 2025, 46(18):37-46.
Li Q, Shao J L, Yin B L, et al. Difference analysis of bioactive components of Yunnan large-leaf tea by different processing technologies[J]. Science and Technology of Food Industry, 2025, 46(18):37-46.
[13] 李胜楠, 赵轩, 陈婷婷. HPLC法测湖北产地多种茶叶的茶水中咖啡因的含量[J]. 广州化工, 2024, 52(3):83-85.
Li S N, Zhao X, Chen T T. Detemination of caffeine cconcentration in tea from Hubei Province by HPLC[J]. Guangzhou Chemical Industry, 2024, 52(3):83-85.
[14] 张媛婷, 罗静, 闫鹏, 等. 基于指纹图谱和化学计量学的茶叶种类鉴别和质量评价[J]. 扬州大学学报(农业与生命科学版), 2024, 45(1):106-113; 146.
Zhang Y T, Luo J, Yan P, et al. Species identification and quality evaluation of tea based on HPLC fingerprint and stoichiometry[J]. Journal of Yangzhou University (Agricultural and Life Science Edition), 2024, 45(1):106-113; 146.
[15] 管梦迪, 郑亚茹, 杨柱林, 等. 甘油提取结合液相色谱-串联质谱法检测茶叶及副产物中7类化学成分[J]. 食品科学, 2024, 45(6):153-163.
Guan M D, Zheng Y R, Yang Z L, et al. Glycerin extraction combined with high performance liquid chromatography-tandem mass spectromet[J]. Food Science, 2024, 45(6):153-163.
[16] 刘培琴, 胡超琼, 邹燕, 等. HPLC-MS/MS法测定茶叶中儿茶素类、茶黄素类物质[J]. 中国测试, 2024, 50(7):63-69.
Liu P Q, Hu C Q, Zou Y, et al. Determination of catechins and theaflavins in tea by HPLC-MS/MS[J]. China Measurement & Testing Technology, 2024, 50(7):63-69.
[17] 杜颖颖, 刘相真, 叶美君, 等. 超高效液相色谱-串联质谱法测定茶叶中游离氨基酸成分[J]. 色谱, 2019, 37(6):597-604.
Du Y Y, Liu X Z, Ye M J, et al. Detection of free amino acids in tea using ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2019, 37(6):597-604.
[18] 卢叶, 张季, 陈艳, 等. 一种使用UPLC-MS/MS同时分析茶叶中多类非挥发性化合物的分析方法及其应用: CN119438469A [P]. 2025-02-14.
Lu Y, Zhang J, Chen Y, et al. An analytical method for simultaneous analysis of multiple non-volatile compounds in tea using UPLC-MS/MS and its application: CN119438469A [P]. 2025-02-14.
[19] Xiao Y, He C, Chen Y L, et al. UPLC–QQQ–MS/MS-based widely targeted metabolomic analysis reveals the effect of solid-state fermentation with Eurotium cristatum on the dynamic changes in the metabolite profile of dark tea[J]. Food Chemistry, 2022, 378:131999.
[20] 刘先军, 冯家力, 曾栋, 等. 超高效液相色谱-串联质谱法测定蜂蜜中18种生物碱及风险评估[J]. 分析测试学报, 2023, 42(11):1517-1523.
Liu X J, Feng J L, Zeng D, et al. Risk assessment and determination of 18 alkaloids in honey by ultra performance liquid chromatography-tandem mass spectrometry[J]. Journal of Instrumental Analysis, 2023, 42(11):1517-1523.
[21] 王强, 汪晨霞, 江蓝蓝, 等. 固相萃取-超高效液相色谱串联质谱法同时测定保健品中5种生物碱[J]. 中国酿造, 2019, 38(6):185-190.
Wang Q, Wang C X, Jiang L L, et al. Simultaneous determination of five alkaloids in health products by SPME-UPLC-MS/MS[J]. China Brewing, 2019, 38(6):185-190.
[22] 张楠, 王雪, 王珂雯, 等. 基于不同提取溶剂和色谱分离模式的芒果浆代谢组学研究[J]. 食品工业科技, 2025, 46(12):10-19.
Zhang N, Wang X, Wang K W, et al. Comparative study of different extraction solvents and liquid chromatography separation modes on metabolomics analysis of mango pulp[J]. Science and Technology of Food Industry, 2025, 46(12):10-19.
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