本文对屎肠球菌Enterococcus faecium EC-12的万古霉素耐药基因进行了筛查。通过PCR方法，以七种典型肠球菌万古霉素耐药基因(vanA、vanB、vanC1、vanC2 /C3、vanD、vanE和vanG)进行特异性扩增，结果均为阴性。PCR方法检测万古霉素耐药肠球菌简便、高效、准确，可用于肠球菌万古霉素耐药基因的快速筛查。

The atrazine-degrading strains were selectedusing high atrazine concentration solid culture and high performance liquid chromatography (HPLC), then the dynamics of growth and atrazine degradation rate were determined, and the effects of co-substrates and environmental condition ondegradation rate were also evaluated. The results showed that 10 strains had degradative capability for atrazinewith the degradation rate from 13.3% to 41.1%, and the maximum degradation rate was obtained with strain XQB-33. Among 10 strains, 4 strains (XQB-1, XQB-21, XQB-25, XQB-33) showed the higher atrazine degradation capacity (38.1%, 34.6%, 37.3% and 41.1%, respectively)．Furthermore, 4 strains with higher degradative capacity reached logarithmic phase, and showed the highest atrazine degradation rate after 72h liquid medium culture. After addition of glucose, sodium citrate, sucrose and succinic acid sodium acetate as co-substrates, the degradation rate of 4 strains increased significantly, especially for 0.5% glucose, the degradation rate of XQB-1 and XQB-1 increased by 15.2% and 14.3%, respectively.The environmental factors can affect the degradation efficiency. The optimum reaction conditions for 4 strains degradation were inoculation amount 2.0%，initial concentration of atrazine 100-200 mg.L-1, initial pH value 5-6, culture temperature at 30℃, and volume at 75-100100mL/150mL.