Abstract: Zearalenone, an estrogen-like mycotoxin produced by strains from Fusarium sp., causes abortion and has estrogenic, teratogenic, carcinogenic and neurotoxic effects. Its contamination affects the safety of animal and plant-derived foods and raw materials seriously. In recent years, the higher sensitive and specific biosensor used for zearalenone detection is developed and applied. Combining related national and international research achievements in the past four years, biosensor detection technologies such as electrochemical biosensors, fluorescent biosensors, aptamer biosensors and cell sensors. used for detecting zearalenone mycotoxins were reviewed. Their sensitivity, specificity and other characteristics during the detection process were emphasized. Meanwhile, the probable problems and directions for future development were discussed.
 GUTUERREZ F A, RUBIANES M D, RIVAS G A. Electrochemical sensor foraminoacids and glucose based on glassy carbon electrodes modified with multi-walled carbon nanotubes and copper microparticles dispersed inpolyethylenimine [J] . Electroanal Chem 2016, 765:16-21.  MASSART F, SAGGESE G. Oestrogenic mycotoxin exposures and precocious pubertal development[J] . International Journal of Andrology, 2010, 33(2):369-376.  HIGGINSON J. International agency for research on cancer[J] . Encyclopedia of Toxicology, 2014, 133(9): 1067-1069.  POOR M, KUNSAGI-MATE S, BALINT M, et al. Interaction of mycotoxin zearalenone with human serum albumin [J] . Journal of Photochemistry & Photobiology B Biology, 2017, 170:16-24.  PIACENTINI K C, ROCHA L O, SAVI G D, et al. Occurrence of deoxynivalenol and zearalenone in brewing barley grains from Brazil [J] . Mycotoxin Res, 2018:1-6.  SAVIG D, PIACENTINI K C, ROCHA L O, et al. Incidence of toxigenic fungi and zearalenone in rice grains from Brazil [J] . International Journal of Food Microbiology, 2018, 270:5-13.  JIE L, SUN L, ZHANG J, et al. Aflatoxin B1, zearalenone and deoxynivalenol in feed ingredients and complete feed from central China [J] . Food Additives & Contaminants Part B Surveillance, 2016, 9(2):91-97.  WU L, LI J, LI Y, et al. Aflatoxin B1, zearalenone and deoxynivalenol in feed ingredients and complete feed from different Province in China [J] . Journal of Animal Science and Biotechnology, 2017, 7(2):428-437.  MA R, ZHANG L, LIU M, et al. Individual and combined occurrence of mycotoxins in feed ingredients and complete feeds in china [J] . Toxins, 2018, 270(3):5-13.  KOVACS M. Nutritional health aspects of mycotoxins [J] . Orv Hetil, 2004,145(34):1739-1746.  European Food Safety Authority (EFSA). Scientific opinion on the risks for public health related to the presence of zearalenone in food [J] . EFSA, 2011, 9(1): 2197-2321.  YIN S T, ZHANG Y Y, GAO R, et al. The inmunomodulatory effects induced by dietary zearalenone in pregnant rats[J] . Immunopharmacol & Immunotoxicol, 2014, 36(3): 187-194.  中华人民共和国卫生部. GB 2761—2017, 食品安全国家标准食品中真菌毒素限量标准[S] . 北京:中国标准出版社, 2017.  RHOUATI A, BULBUL G, LATIF U, et al. Nano-Aptasensing in mycotoxin analysis: Recent updates and progress [J] . Toxins, 2017, 9(11):349.  ARDUINI F, AMINE A, MOSCONE D, et al. Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B 1, detection (review) [J] . Microchimica Acta, 2010, 170(3-4):193-214.  BUSMAN C M M M. Rapid and advanced tools for mycotoxin analysis: A review [J] . Food Addit Contam Part A Chem Anal Control Expo Risk Assess, 2010, 27(5):688-700.  YAO H, HRUSKA Z, MAVUNGU J D. Developments in detection and determination of aflatoxins [J] . World Mycotoxin Journal, 2015, 8(2):181-191.  谢顺碧,袁若. 电化学生物传感器中的信号放大技术的研究进展[J] . 化学传感器, 2016, 36(2):11-25.  LIU L, CHAO Y, CAO W, et al. A label-free amperometric immunosensor for detection of zearalenone based on trimetallic Au-core/AgPt-shell nanorattles and mesoporous carbon [J] . Analytica Chimica Acta, 2014, 847:29-36.  AFZALI D, FATHIRAD F. Determination of zearalenone with a glassy carbon electrode modified with nanocomposite consisting of palladium nanoparticles and a conductive polymeric ionic liquid [J] . Microchimica Acta, 2016, 183(9):1-6.  AFZALI D, PADASH M, MODTAFAVI A. Determination of trace amounts of zearalenone in beverage samples with an electrochemical sensor [J] . Mycotoxin Research, 2015, 31(4):1-6.  RIBERI W I, TARDITTO L V, ZON M A, et al. Development of an electrochemical immunosensor to determine zearalenone in maize using carbon screen printed electrodes modified with multi-walled carbon nanotubes/polyethyleneimine dispersions [J] . Sensors & Actuators B Chemical, 2017, 254:1271-1277.  LIU N, NIE D, TAN Y, et al. An ultrasensitive amperometric immunosensor for zearalenones based on oriented antibody immobilization on a glassy carbon electrode modified with MWCNTs and AuPt nanoparticles [J] . Microchimica Acta, 2016, 184(1):1-7.  SADRABADI N R, ENSAFI A A, HEYDARI-BAFROOEI E, et al. Screening of food samples for zearalenone toxin using an electrochemical bioassay based on DNA-zearalenone interaction [J] . Food Analytical Methods, 2016, 9(9):2463-2470.  XU W, YING Q, CHEN S, et al. Electrochemical indirect competitive immunoassay for ultrasensitive detection of zearalenone based on a glassy carbon electrode modified with carboxylated multi-walled carbon nanotubes and chitosan[J] . Microchimica Acta, 2017,184(9): 3339-3347.  ZHAO W W, XU J J, CHEN H Y. Photoelectrochemical bioanalysis: the state of the art [J] . Chemical Society Reviews, 2015, 44(3):729-741.  LIU N, CHEN S, LI Y, et al. Self-enhanced photocathodic matrix based on poly-dopamine sensitized TiO2mesocrystals for mycotoxin detection assisted by a dual amplificatory nanotag [J] . New Journal of Chemistry, 2017, 41(9):3380-3386.  ZHU Q, CAI F, ZHANG J, et al. Highly sensitive electrochemiluminescent immunosensor based on gold nanoparticles-functionalized zinc oxide nanorod and poly(amidoamine)-graphene for detecting brombuterol [J] . Biosensors & Bioelectronics, 2016, 86:899-906.  ZHANG X, WANG X, SUN M, et al. A magnetic nanoparticle based enzyme-linked immunosorbent assay for sensitive quantification of zearalenone in cereal and feed samples [J] . Toxins, 2015, 7(10):4216-4231.  LIU J, HU Y, ZHU G, et al. Highly sensitive detection of zearalenone in feed samples using competitive surface-enhanced raman scattering immunoassay [J] . Journal of Agricultural & Food Chemistry, 2014, 62(33):8325-8332.  EDUPUGANTI S R, EDUPUGANTI O P, O’KENNEDY R. Generation of anti-zearalenone scFv and its incorporation into surface plasmon resonance-based assay for the detection of zearalenone in sorghum[J] . Food Control, 2013, 34(2):668-674.  HOSSAIN M Z, MARAGOS C M. Gold nanoparticle-enhanced multiplexed imaging surface plasmon resonance (iSPR) detection of Fusarium mycotoxins in wheat [J] . Biosensors & Bioelectronics, 2017, 101:245-252.  JOSHI S, SEGARRA-FAS A, PETERS J, et al. Multiplex surface plasmon resonance biosensing and its transferability towards imaging nanoplasmonics for detection of mycotoxins in barley [J] . Analyst, 2016, 141(4):1307-1318.  WANG Zhan-hui, LI Cheng-long, WEN Kai, et al. A universal multi-wavelength fluorescence polarization immunoassay for multiplexed detection of mycotoxins in maize [J] . Biosensors & Bioelectronics, 2016, 79:258-165.  ZHANG X, ERMIN S A, WEN K, et al. Fluorescence polarization immunoassay based on a new monoclonal antibody for the detection of the zearalenone class of mycotoxins in maize [J] . Journal of Agricultural & Food Chemistry, 2017, 65(10):2240-2247.  LIU N, NIE D X, WU A B, et al. Ultrasensitive immunoassays based on biotin-streptavidin amplified system for quantitative determination of family zearalenones [J] . Food Control, 2015, 57:202-209.  ZHAO F, SHEN Q, WANG H, et al. Development of a rapid magnetic bead-based immunoassay for sensitive detection of zearalenone [J] . Food Control, 2017,79:227-233.  HENDRICKSON O D, CHERTOCIVH J O, ZHERDEV A V, et al. Ultrasensitive magnetic ELISA of zearalenone with pre-concentration and chemiluminescent detection [J] . Food Control, 2017,84:330-338.  ZHAN S, HUANG X, CHEN R, et al. Novel fluorescent ELISA for the sensitive detection of zearalenone based on H2O2-sensitive quantum dots for signal transduction [J] . Talanta, 2016, 158:51-56.  ZHANG X, WANG X, SUN M, et al. A magnetic nanoparticle based enzyme-linked immunosorbent assay for sensitive quantification of zearalenone in cereal and feed samples[J] . Toxins, 2015, 7(10):4216-4231.  WANG Y K, ZOU Q, SUN J H, et al. Screening of single-stranded DNA (ssDNA) aptamers against a zearalenone monoclonal antibody and development of a ssDNA-based enzyme-linked oligonucleotide assay for determination of zearalenone in corn [J] . Journal of Agricultural & Food Chemistry, 2015, 63(1):136-141.  WU Z, XU E, CHUGHTAI M F J, et al. Highly sensitive fluorescence sensing of zearalenone using a novel aptasensor based on upconverting nanoparticles[J] . Food Chemistry, 2017, 230:673-680.  NIAZ S, WANG X, PASHA I, et al. A novel bioassay based on aptamer-functionalized magnetic nanoparticle for the detection of zearalenone using time resolved-fluorescence NaYF 4: Ce/Tb nanoparticles as signal probe [J] . Talanta, 2018, 186(15): 97-103.  TAGHDISI S M, DANESH N M, RAMEZANI M, et al. Novel colorimetric aptasensor for zearalenone detection based on nontarget-induced aptamer walker, gold nanoparticles, and exonuclease-assisted recycling amplification [J] . ACS applied materials & interfaces, 2018,10 (15): 12504-12509.  GOUD K Y, HAYAT A, SATYANARAYANA M, et al. Aptamer-based zearalenone assay based on the use of a fluorescein label and a functional graphene oxide as a quencher [J] . Microchimica Acta, 2017, 184(11):1-8.  JI J, GU W, SUN C, et al. A novel recombinant cell fluorescence biosensor based on toxicity of pathway for rapid and simple evaluation of DON and ZEN [J] . Scientific Reports, 2016, 6:31270.  DU Wen-shu, ZHU Pei, JIANG Dong-lei, et al. A novel and simple cell-based electrochemical impedance biosensor for evaluating the combined toxicity of DON and ZEN[J] . Biosensors & Bioelectronics, 2015, 70:447-454.  SUN Xiu-lan, XIA Shuang, ZHU Pei, et al. Development of a simple and convenient cell-based electrochemical biosensor for evaluating the individual and combined toxicity of DON, ZEN, and AFB1[J] . Biosensors & Bioelectronics, 2017, 97:345-351.