Abstract: The experiment was carried out using dilution coating method to coat the mud of the Yellow Crane Tower Luzhou-flavor liquor. Plate scribing purification, microscopic examination, and single-bacteria fermentation were conducted, followed by using gas chromatography-mass spectrometer (GC-MS) to screen functional bacteria with high yield of guaiacol. The results of molecular biology techniques showed that two functional strains, B-1 and B-3, with high-yield of guaiacol were Bacillus amyloliquefaciens and Delfitia sp. X-a12, respectively. Their total yields were 3 632.33 μg/L and 14 527.31 μg/L, respectively. The tolerance analysis results showed that both strains were resistant to 4% vol. alcohol, pH=4.5, and 15% sugar.
周金虎,李良,方尚玲,等. 窖泥中高产愈创木酚类功能菌的筛选与鉴定[J]. 食品与发酵工业, 2019, 45(3): 64-70.
ZHOU Jinhu,LI Liang,FANG Shangling,et al. Screening and identification of functional boateria with high yieldguaiacol in the mud[J]. Food and Fermentation Industries, 2019, 45(3): 64-70.