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食品与发酵工业  2019, Vol. 45 Issue (11): 65-70    DOI: 10.13995/j.cnki.11-1802/ts.019872
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
胶红酵母Rhodotorula mucilaginosa CICC 33013胞外多糖的分离纯化及抗氧化活性研究
马文锦*, 李梅林, 王博, 张永显, 于长青, 班省华
(甘肃省轻工研究院有限责任公司,甘肃 兰州,730030)
Isolation, purification and antioxidant activities of extracellular polysaccharidesfrom Rhodotorula mucilaginosa
MA Wenjin*, LI Meilin, WANG Bo, ZHANG Yongxian, YU Changqing, BAN Shenghua
(Gansu Institute of Light Industry Limited Liability Company, Lanzhou 730030, China)
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摘要 研究胶红酵母Rhodotorula mucilaginosa CICC 33013菌株胞外多糖的初级结构与抗氧化活性。分离纯化得到胞外多糖的单一组分REPS2-A,分析其分子质量分布及自由基清除活性。结果表明,多糖组分REPS2-A的分子质量为7.125×106 Da,质量浓度为8 mg/mL时,DPPH自由基清除率为49.1%,ABTS自由基清除率为51.2%,还原力为0.352。该文为了解胶红酵母菌的胞外多糖结构与功能提供了新的数据依据,为后续胶红酵母菌胞外多糖的构-效关系研究提供了实验基础。
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马文锦
李梅林
王博
张永显
于长青
班省华
关键词:  胶红酵母  胞外多糖  结构解析  抗氧化    
Abstract: The primary structures and antioxidant activities of Rhodotorula mucilaginosa extracellular polysaccharides were studied in this paper. A single component REPS2-A in extracellular polysaccharides was obtained by isolation and purification, followed by measuring its molecular weight distribution and free radical scavenging activities. The results showed that the molecular weight of REPS2-A was 7.125×106 Da. Moreover, the scavenging rates of DPPH and ABTS free radicals by 8 mg/mL REPS2-A were 49.1% and 51.2%, respectively, and the reducing power was 0.352. This paper provides a new data basis for understanding the structures and functions of extracellular polysaccharides of R. mucilaginosa, which provides an experimental basis for subsequent studies on correlations between their structures and activities.
Key words:  Rhodotorula mucilaginosa    extracellular polysaccharide    structure analysis    antioxidant
收稿日期:  2019-01-07                出版日期:  2019-06-15      发布日期:  2019-07-08      期的出版日期:  2019-06-15
基金资助: 甘肃省重点研发计划(18YF1FA012);西北特色农畜产品深加工国家地方联合工程研究中心项目(发改高技[2015]3173号);甘肃特色农产品深加工技术创新中心项目(甘科计[2018]23号)
通讯作者:  博士,高级工程师(E-mail:mawj0707@163.com)   
引用本文:    
马文锦,李梅林,王博,等. 胶红酵母Rhodotorula mucilaginosa CICC 33013胞外多糖的分离纯化及抗氧化活性研究[J]. 食品与发酵工业, 2019, 45(11): 65-70.
MA Wenjin,LI Meilin,WANG Bo,et al. Isolation, purification and antioxidant activities of extracellular polysaccharidesfrom Rhodotorula mucilaginosa[J]. Food and Fermentation Industries, 2019, 45(11): 65-70.
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http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.019872  或          http://sf1970.cnif.cn/CN/Y2019/V45/I11/65
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