Tolerance of Enterococcus durans C11 to environmental stresses andgenomic analysis of genes related to low-temperature adaptation
YAO Wenting1,2, CHEN Lanming1,2*
1(College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306,China) 2(Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage andPreservation, Ministry of Agriculture (Shanghai), Shanghai 201306,China)
Abstract: This study was constructed to dissect the tolerance of Enterococcus durans C11 to environmental stresses and possible molecular mechanisms of its ability to adapt low-temperature environment. Tolerance of E. durans C11 was determined under different temperatures, acidities, osmolality, and artificial gastrointestinal fluids, and comparative genomic analysis was also carried out. The results showed that E. durans C11 was able to grow at 15-25 ℃, but had no significant tolerances to acid (pH 2.0-4.0), osmotic pressure induced by 5.0%-15.0% NaCl and 0.05%-0.3% bile salt, as well as to artificial gastric and intestinal fluids. It was also revealed that E. durans C11 had 33 strain-specific genes, which encoded for 26 hypothetical proteins and 7 functional proteins. Moreover, a number of genes involved in synthesizing cell membrane unsaturated fatty acids, uptaking or synthesizing compatible solutes, and regulating environmental stresses were identified from E. durans C11 genome, which may be related to the low-temperature adaptation of the bacterium. This study provides some data supports for future study on low-temperature adaptation of E. durans.
姚文婷,陈兰明. 耐久肠球菌C11菌株的环境胁迫耐受性及其低温适应相关基因的基因组学鉴定[J]. 食品与发酵工业, 2019, 45(11): 21-28.
YAO Wenting,CHEN Lanming. Tolerance of Enterococcus durans C11 to environmental stresses andgenomic analysis of genes related to low-temperature adaptation[J]. Food and Fermentation Industries, 2019, 45(11): 21-28.
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2019, Vol. 45 
