Abstract: This study aimed to obtain an economical, effective and rapid method to synchronously extract Cyclocarya paliurus polysaccharides and flavonoids to improve the extraction rate and utilization rate of raw materials to the greatest extent. Microbial fermentation technology was used to extract Cyclocarya paliurus polysaccharides and flavonoids, and the process was optimized by single factor and orthogonal tests. The results showed the optimal extraction process was as follows: mixed Cyclocarya paliurus leave and water in a solid to liquid ratio of 1∶20, added 0.4% (w/w) cellulase and hemicellulase composite enzyme, followed by inoculating 5.0% seed liquid that consisted of Aspergillus niger and Bacillus subtilis in a ratio of 1∶2, and fermented at 160 r/min and 30 ℃ for 2 d. Under this condition, the yield of Cyclocarya paliurus polysaccharides was 7.57%, which was 50.50% higher than that before optimization. Moreover, the yield of flavonoids was 6.98%, which increased by 49.46% in comparison to the control. Therefore, microbial fermentation technology can greatly improve Cyclocarya paliurus polysaccharides and flavonoids extraction yields, which provides a theoretical basis for developing Cyclocarya paliurus functional products.