Abstract: A method for the simultaneous determination of 18 sulfonamides in antler velvet of Cervus by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed by using the pretreatment technology of dispersed solid-phase extraction. Antler samples were extracted with acetonitrile and purified with 70 mg PSA, 100 mg C18 and 50 mg neutral alumina. The UPLC analysis was carried out by a 35 ℃ thermostatic Waters Acquity UPLC BEH C18 column(2.1 mm×100 mm,1.7 μm)with a mobile phase composed of 25 mmol/L formic acid acetonitrile-water for gradient elution at 0.3 mL/min. The detection was carried out on MS/MS with ESI in positive ion in the multiple reaction monitoring (MRM) mode. The content of sulfonamides in anter velvet was quantified by external standard method. The results showed that 18 varieties of sulfonamides had a good linear relationship between 0.025-50 ng/mL; the correlation coefficient R2 were all greater than 0.999 1; limit of detection (LOD) of 7 kinds of sulfonamides was 0.025 μg/kg; limit of quantitation (LOQ) was 0.075 μg/kg; LOD of 11 kinds of sulfonamides was 0.075 μg/kg; and LOQ was 0.25 μg/kg. The average recovery of 18 sulfonamides was 80.4%-107.2% with relative standard deviation (RSD) less than 5%. The method has the advantages of simple operation, remarkable purification effect, high sensitivity, good accuracy and low detection limit. It can be used for the determination of 18 sulfonamides in antler velvet of Cervus Nippon Temminck.