Abstract: In order to realize food waste-converted animal feed utilization and storage, an anaerobic fermentation process was developed. The public kitchen waste samples fermented by mixed microorganisms were divided into four treatment groups: A: acidification without sterilization, B: acidification with sterilization, C: pickle water without sterilization and D: pickle water with sterilization. The four groups were subjected to anaerobic fermentation at room temperature for 14 days, and the amount of the harmful microorganisms, lactic acid, water, fat, protein, and cellulose, as well as their pH during the fermentation process were determined. The results showed that pathogenic microorganisms, such as Staphylococcus aureus and Salmonella sp., were not detected in any of the group, and the pH values of A, B, C, and D group were 3.38, 3.38, 3.34 and 3.39, respectively. The lactic acid concentrations were 1.19%, 0.43%, 0.90% and 0.73%, respectively. Among them, the A group (acidification without sterilization-treated fermentation) had the best effect.