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食品与发酵工业  2020, Vol. 46 Issue (4): 57-64    DOI: 10.13995/j.cnki.11-1802/ts.022514
  研究报告 本期目录 | 过刊浏览 | 高级检索 |
乳酸菌对发酵牦牛肉灌肠理化性质及挥发性风味物质的影响
龚珏, 唐善虎*, 李思宁, 郑娇, 谭雪梅
(西南民族大学 生命科学与技术学院, 四川 成都, 610041)
Effect of lactic acid bacteria on physicochemical properties and volatile flavor substances of fermented yak meat sausage
GONG Jue, TANG Shanhu*, LI Sining, ZHENG Jiao, TAN Xuemei
(College of Life Science and Technology, Southwest Minzu University, Chengdu 610041,China)
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摘要 该文以自然发酵为对照,研究了清酒乳杆菌(Lactobacillus sakei,LS)、植物乳杆菌(Lactobactillus plantarum,LP)和戊糖片球菌(Pediocccus pentosaceus,PP)作为发酵剂对牦牛肉灌肠理化性质及挥发性风味物质的影响。测定牦牛肉灌肠发酵过程中的pH值、水分含量、色泽、挥发性盐基氮(total voatile base nitrogen,TVB-N)、质构(texture,TPA)以及挥发性风味物质,探究乳酸菌发酵剂对牦牛肉灌肠理化指标及挥发性风味物质的影响。结果表明,随着发酵时间的延长,pH值和水分含量快速下降,接种乳酸菌的处理组下降速度更快,其中pH值最终都降至5.2以下,水分含量降至20%左右;接种了乳酸菌处理组的TVB-N要显著低于对照组(P<0.05);PP组的L*值、a*值、b*值均显著高于其他组(P<0.05);LS、LP组则检测出更大的硬度、黏聚性和咀嚼性;挥发性物质测定结果显示接种乳酸菌的处理组有更多种、更高相对含量的醛类、酮类等物质被检出。接种功能性乳酸菌发酵制作牦牛肉发酵灌肠在一定程度上改善香肠理化特性,同时能促进挥发性风味物质的生成。
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龚珏
唐善虎
李思宁
郑娇
谭雪梅
关键词:  发酵灌肠  牦牛肉  乳酸菌  理化性质  挥发性风味物质    
Abstract: The effects of Lactobacillus sakei (LS), Lactobacillus plantarum (LP) and Pediococcus pentosaceus (PP) as starter culture on the physicochemical properties and volatile flavor substances of yak meat sausage were studied and compared with naturally fermented control group. The pH value, moisture content, color, total volatile base nitrogen (TVB-N), texture (TPA) and volatile flavor substances in the fermentation process of yak meat sausage were determined which in order to explore the effects of lactic acid bacteria on the quality of yak meat sausage. The results showed that with the prolongation of fermentation time, the pH value and moisture content decreased rapidly. The pH value and moisture content of the groups inoculated with lactic acid bacteria decreased faster than those of the control group. The pH value eventually fell to 5.2, and the moisture content decreased about 20%. The TVB-N content in the lactic acid bacteria-treated groups were significantly lower than that of the control group (P<0.05). The L* value, a* value and b* value of the PP group were significantly higher than those of the other groups (P<0.05). LS and LP groups had greater hardness, cohesiveness and chewiness. Higher contents of aldehydes, ketones and other substances of volatile substances were detected in the groups inoculated with lactic acid bacteria. Fermentation with lactic acid bacteria can improve the physicochemical properties and promote the formation of volatile flavor substances of yak meat sausage.
Key words:  fermented sausage    yak meat    lactic acid bacteria    physicochemical properties    volatile flavor substances
收稿日期:  2019-10-11                出版日期:  2020-02-25      发布日期:  2020-04-07      期的出版日期:  2020-02-25
基金资助: 国家重点研发计划(2018YFD0400101);研究生创新项目(CX2019SZ150)
作者简介:  硕士研究生(唐善虎教授为通讯作者,E-mail:stang01@126.com)
引用本文:    
龚珏,唐善虎,李思宁,等. 乳酸菌对发酵牦牛肉灌肠理化性质及挥发性风味物质的影响[J]. 食品与发酵工业, 2020, 46(4): 57-64.
GONG Jue,TANG Shanhu,LI Sining,et al. Effect of lactic acid bacteria on physicochemical properties and volatile flavor substances of fermented yak meat sausage[J]. Food and Fermentation Industries, 2020, 46(4): 57-64.
链接本文:  
http://sf1970.cnif.cn/CN/10.13995/j.cnki.11-1802/ts.022514  或          http://sf1970.cnif.cn/CN/Y2020/V46/I4/57
[1] LUO X L, TONG Z B, WEI Y P, et al. Meat characteristics of Qinghai yak and semi-wild yak[J]. Animal Science Journal, 2010, 77(2):230-234.
[2] 拜彬强,郝力壮,柴沙驼,等.牦牛肉品质特性研究进展[J].食品科学,2014,35(17):290-296.
[3] YIN R H, BAI W L, WANG J M, et al. Development of an assay for rapid identification of meat from yak and cattle using polymerase chain reaction technique[J]. Meat Science, 2009, 83(1):38-44.
[4] 侯丽,柴沙驼,刘书杰,等.青海牦牛肉与秦川牛肉食用品质和加工品质的比较研究[J].食品科学,2013,34(11):49-52.
[5] 王俊钢,李开雄,韩冬印.国内外发酵香肠的研究现状及其展望[J].肉类工业,2009(6):49-51.
[6] HAMMES W P, HERTELl C. New developments in meat starter cultures[J]. Meat Science, 1998, 49:S125-S138.
[7] 龙强,聂乾忠,刘成国.发酵香肠研究进展及展望[J].食品科学,2017,38(13):291-298.
[8] 潘晓倩,成晓瑜,张顺亮,等.不同发酵剂对北方风干香肠色泽和风味品质的改良作用[J].食品科学,2015,36(14):81-86.
[9] CHEN Q, LIU Q, SUN Q, et al. Flavour formation from hydrolysis of pork sarcoplasmic protein extract by a unique LAB culture isolated from Harbin dry sausage[J]. Meat Science, 2015, 100:110-117.
[10] 丁希,唐善虎,李思宁,等.恒温发酵温度对发酵牦牛肉灌肠理化性质的影响[J].食品工业科技,2019,40(5):42-47.
[11] MALLE P,POUMEYROL M. A new chemical criterion for the quality control of fish: Trimethylamine/total volatile basic nitrogen(%)[J]. Journal of Food Protection, 1989, 52(6):419-423.
[12] STOLKERA A M, LINDERS S H M A, VANGINKEL L A, et al. Application of the revised EU criteria for the confirmation of anabolic steroids in meat using GC-MS[J]. Analytical and bioanalytical chemistry, 2004, 378(5):1 313-1 321.
[13] 陈倩,韩齐,孔保华,等.乳酸菌对发酵肉制品中肌肉蛋白降解作用的研究进展[J].食品科学,2014,35(9):279-284.
[14] XU Y, XIA W, YANG F, et al. Effect of fermentation temperature on the microbial and physicochemical properties of silver carp sausages inoculated with Pediococcus pentosaceus[J]. Food Chemistry, 2010, 118(3):512-518.
[15] 陈曦,许随根,周彤,等.贵州酸肉中的植物乳杆菌对发酵香肠风味和品质特性的影响[J].中国食品学报,2018,18(6):174-182.
[16] GØTERUP J, OLSEN K, KNØCHEL S, et al. Color formation in fermented sausages by meat-associated staphylococci with different nitrite- and nitrate reductase activities[J]. Meat Science, 2008, 78(4):492-501.
[17] 韦诚,常荣,段珍珍,等.发酵时长对酸肉物理特性及食用安全性的影响[J].食品与发酵工业,2019,45(8):86-92.
[18] WANG Y, SUN Y, ZHANG X, et al. Bacteriocin-producing probiotics enhance the safety and functionality of sturgeon sausage[J]. Food Control, 2015, 50:729-735.
[19] 吴雪燕,张培培,汪淼,等.中式香肠蛋白氧化降解对产品品质的影响[J].食品工业科技,2014,35(14):151-156.
[20] MASSIMILIANO S, DELTORE M, STECCHINI M. Changes of physicochemical, microbiological, and textural properties during ripening of Italian low-acid sausages. Proteolysis, sensory and volatile profiles[J]. Meat Science, 2009, 81(1):77-85.
[21] GIMENO O, ANSORENA D, ASTIASARÁN I, et al. Characterization of chorizo de Pamplona: Instrumental measurements of colour and texture[J]. Food Chemistry, 2000, 69(2):195-200.
[22] RAHMAN M S, AL-FARSI S A. Instrumental texture profile analysis (TPA) of date flesh as a function of moisture content[J]. Journal of Food Engineering, 2005, 66(4):505-511.
[23] 曹辰辰,冯美琴,孙健,等.功能性发酵剂对发酵香肠氧化稳定性及挥发性风味物质的影响[J].食品科学,2019,40(20):106-113.
[24] YANG Y, ZHANG X, WANG Y, et al. Study on the volatile compounds generated from lipid oxidation of Chinese bacon (unsmoked) during processing: Aroma generated from lipid oxidation in bacon (unsmoked)[J]. European Journal of Lipid Science and Technology, 2017, 119(10):1 600 512.
[25] SIDIRA M, KANDYLIS P, KANELLAKI M, et al. Effect of immobilized Lactobacillus casei on the evolution of flavor compounds in probiotic dry-fermented sausages during ripening[J]. Meat Science, 2015,100: 41-51.
[26] OLIVARES A, NAVARRO J L, FLORES M. Establishment of the contribution of volatile compounds to the aroma of fermented sausages at different stages of processing and storage[J]. Food Chemistry, 2009, 115(4):1 464-1 472.
[27] 温荣欣,扈莹莹,殷小钰,等.食盐添加量对哈尔滨风干肠脂质和蛋白氧化及挥发性化合物形成的影响[J/OL].食品科学:1-13[2019-11-13].http://kns.cnki.net/kcms/detail/11.2206.TS.20190514.1629.002.html.
[28] MATEO J, ZUMALACÁRREGUI J M. Volatile compounds in chorizo and their changes during ripening[J]. Meat Science, 1996, 44(4):255-273.
[29] LARROUTURE C, ARDAILLON V, PÉPIN M, et al. Ability of meat starter cultures to catabolize leucine and evaluation of the degradation products by using an HPLC method[J]. Food Microbiology, 2000, 17(5):563-570.
[30] 要萍,马长伟,周珊,等.运用顶空固相微萃取技术分析宣威火腿中的挥发性化合物[J].食品与发酵工业,2003, 29(10):1-5.
[31] 王恺,慕妮,李亮,等.不同发酵剂对发酵香肠挥发性风味物质的影响[J].食品工业技,2013,34(14):177-181;196.
[32] 常海军,周文斌,朱建飞.重庆城口香肠挥发性风味成分的分离与鉴定[J].食品科学,2016,37(6):146-152.
[33] 谢丽源,郑林用,彭卫红,等.基于主成分分析法对不同温度处理下杏鲍菇的品质评价[J].食品与发酵工业,2016,42(6):80-85.
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