Abstract: The low-methoxy pectin was extracted from Premna microphylla Turcz with ultrasound-enzyme method. Its physicochemical properties, structural characteristics, antioxidant activity and rheological properties were explored. The results showed that the yield of pectin extracted by ultrasonic-enzyme method was 35.53%, which was 17.28% higher than that of the traditional acid extraction method. The pectin obtained belonged to low-ester pectin (the degree of esterification was 16.80%). The color of the pectin was pale yellow, and its galacturonic acid content was 81.17%. Molecular weight of the pectin was determined by HPLC. The weight average molecular weight (Mw) was 27.24 kDa, the number average molecular weight (Mn) was 15.30 kDa, and the Mw/Mn was 1.78, which indicated that the molecular weightof P. microphylla pectin was relatively low and uniform. D-Galacturonic acid, glucose, rhamnose and galactose were the main monosaccharide components of the pectin. Its structural linearity was low and its main structure was RG-I Type with short side chain, which were found through the analysis of monosaccharide composition. Characteristic peaks of Fourier transform infrared spectrometer confirmed that it contained pectin. SEM showed that the surface was rough and contained a lot of pore structure. X-ray diffraction analysis showed that the pectin had a certain degree of crystallinity. Differential scanning calorimetry and thermogravimetric analysis results showed that pectin had good thermal stability, and the pectin was degraded at 259.3 ℃. Rheological results showed that pectin extracted from P. microphylla presented non-Newtonian shear-thinning behavior. G' of 10 g/L pectin solution was higher than G″, showing better elasticity. IC50 of DPPH and ABTS free radicals were 0.37 and 0.34 g/L respectively in the antioxidant activity test. This research results provide a theoretical basis for the development and comprehensive utilization of low-ester pectin from P. microphylla.
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