Abstract: The pollution problem in red fermented rice (RFR) fermentation cannot be ignored, since it will affect the production and quality of RFR. We found that RFR inoculation fermentation (with Monacus purpureus) was more contaminated by Aspergillus flavus than natural fermentation. However, pollution does not often occur in RFR fermentation when inoculated with commercially available RFR. It was speculated that the open production of commercially available RFR may introduce certain inhibitors that can inhibit A. flavus. To reduce the occurrence of contamination caused by A. flavus, a Bacillus velezensis strain Y5 that can inhibit the growth of A. flavus spores was isolated from commercially available RFR, and the fermentation conditions for improving the resistance to A. flavus contamination in fermentation of RFR were optimized. Confirmatory experiment results showed that inoculation of strain Y5 with small amount in the early stage of RFR fermentation could effectively reduce the contamination rate of A. flavus under extensive condition and would not affect M. purpureus to produce pigment. Hence, this strain can be used as an auxiliary strain in RFR fermentation to ensure the smooth fermentation.
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