Abstract: In this paper,shrimp meat infected Listeria monocytogenes was treated by phage ListexTMP100 at room temperature(20℃),cold(4℃,0℃) and frozen(-18℃) in order to investigate the reduction of Listeria monocytogenes in shrimp meat during storage.The population of Listeria monocytogenes in shrimp meat using plate count method.The results showed that phage ListexTMP100 can effectively reduce the population of Listeria monocytogenes in shrimp meat when it's concentration was more than 2×107 PFU/mL(P<0.05).The population of Listeria monocytogenes declined 1.5 log10CFU/g at 20℃ for one hour by treated phage ListexTMP100(2×107pfu/mL).The death rate of Listeria monocytogenes reached 99.9% at 4℃ and 0℃ and the number declined to 1.38Log10CFU/g at-18℃ for 24 hours at the same concentration of phage.These results showed that phage ListexTMP100 is a good biological antibacterial agent against Listeria monocytogenes in shrimp meat which makes it an ideal potential biological agent to improve the safety of fishery products for consumption.