Abstract: Antioxidant peptides show great potential in the fields of food,health products,medicine and cosmetics.In this paper,the preparation,purification,structure identification,molecular modification,antioxidant activity evaluation and mechanism of antioxidant peptides were reviewed,the applications of in silico tools in the research of antioxidant peptides were analyzed and summarized.The problems existing in the research and development of antioxidant peptides were pointed out,and the development prospect of antioxidant peptides was outlooked,aiming to provide a reference for their further development and utilization.
[1] LI S,TAN H Y,WANG N,et al.The role of oxidative stress and antioxidants in liver diseases[J].International Journal of Molecular Sciences,2015,16(11):26 087-26 124. [2] ESFANDI R,WALTERS M E,TSOPMO A.Antioxidant properties and potential mechanisms of hydrolyzed proteins and peptides from cereals[J].Heliyon,2019,5(4):e01 538. [3] SHEN R,LIU D S,HOU C C,et al.Protective effect of Potentilla anserina polysaccharide on cadmium-induced nephrotoxicity in vitro and in vivo[J].Food &Function,2017,8(10):3 636-3 646. [4] JIN J E,AHN C B,JE J Y.Purification and characterization of antioxidant peptides from enzymatically hydrolyzed ark shell (Scapharca subcrenata)[J].Process Biochemistry,2018,72:170-176. [5] CHEN Z,BERTIN R,FROLDI G.EC50estimation of antioxidant activity in DPPH center dot assay using several statistical programs[J].Food Chemistry,2013,138(1):414-420. [6] FENG Y X,RUAN G R,JIN F,et al.Purification,identification,and synthesis of five novel antioxidant peptides from Chinese chestnut (Castanea mollissima Blume) protein hydrolysates[J].Lwt-Food Science and Technology,2018,92:40-46. [7] ZENG Y Y,JIAN H Q.Comparison of antioxidant activities of melanin fractions from chestnut shell[J].Molecules,2016,21(4):487. [8] ZENG W C,SUN Q,ZHANG W H,et al.Antioxidant activity in vivo and biological safety evaluation of a novel antioxidant peptide from bovine hair hydrolysates[J].Process Biochemistry,2017,56:193-198. [9] MINKIEWICZ P,IWANIAK A,DAREWICZ M.BIOPEP-UWM database of bioactive peptides:Current opportunities[J].International Journal of Molecular Sciences,2019,20(23):5 978. [10] ASHAOLU T J.Antioxidative peptides derived from plants for human nutrition:Their production,mechanisms and applications[J].European Food Research and Technology,2020,246(5):853-865. [11] BORRAJO P,PATEIRO M,BARBA F J,et al.Antioxidant and antimicrobial activity of peptides extracted from meat by-products:A review[J].Food Analytical Methods,2019,12(11):2 401-2 415. [12] 郑晓吉,许程剑,牛博楠,等.新疆阿魏菇抗氧化肽分离纯化及抗氧化性质[J].食品与生物技术学报,2015,34(5):524-529. ZHENG X J,XU C J,NIU B N,et al.Separation,purification and antioxidant activity study of antioxidant peptides from Pleurotus ferulae lenzi in Xinjiang[J].Journal of Food Science and Biotechnology,2015,34(5):524-529. [13] 栗铭鸿,姜伊悦,张小勇,等.酶法制备元蘑蛋白肽及其分级组分抗氧化活性研究[J].食品与机械,2019,35(11):176-181. LI M H,JIANG Y Y,ZHANG X Y,et al.Preparation and antioxidant activity of protein peptides by enzymatic from Hohenbuehelia serotina[J].Food &Machinery,2019,35(11):176-181. [14] 李桂峰,王向东,赵国建,等.酶解双孢菇蛋白制备抗氧化肽的研究[J].中国食品学报,2011,11(5):37-43. LI G F,WANG X D,ZHAO G J,et al.Enzymolysis technology for preparation of antioxidant peptides from Agaricus bisporus protein[J].Journal of Chinese Institute of Food Science and Technology,2011,11(5):37-43. [15] WONG F C,XIAO J B,WANG S Y,et al.Advances on the antioxidant peptides from edible plant sources[J].Trends in Food Science &Technology,2020,99:44-57. [16] PHONGTHAI S,RAWDKUEN S.Fractionation and characterization of antioxidant peptides from rice bran protein hydrolysates stimulated by in vitro gastrointestinal digestion[J].Cereal Chemistry,2020,97(2):316-325. [17] ZHANG J,WEN C,LI C,et al.Antioxidant peptide fractions isolated from wheat germ protein with subcritical water extraction and its transport across Caco-2 cells[J].Journal of Food Science,2019,84(8):2 139-2 146. [18] ZHANG S,ZHANG M,YANG R,et al.Preparation,identification,and activity evaluation of antioxidant peptides from protein hydrolysate of corn germ meal[J].Journal of Food Processing and Preservation,2019,43(10):e14 160. [19] BALLATORE M B,DEL R B M,BRABER N L V,et al.Antioxidant and cytoprotective effect of peptides produced by hydrolysis of whey protein concentrate with trypsin[J/OL].Food Chemistry,2020.DOI:10.1016/j.foodchem.2020,319:126472. [20] YAGHOUBZADEH Z,GHADIKOLAII F P,KABOOSI H,et al.Antioxidant activity and anticancer effect of bioactive peptides from rainbow trout (Oncorhynchus mykiss) skin hydrolysate[J].International Journal of Peptide Research and Therapeutics,2020,26(1):625-632. [21] YANG X R,QIU Y T,ZHAO Y Q,et al.Purification and characterization of antioxidant peptides derived from protein hydrolysate of the marine bivalve mollusk Tergillarca granosa[J].Marine Drugs,2019,17(5):251. [22] ADMASSU H,GASMALLA M A A,YANG R J,et al.Bioactive peptides derived from seaweed protein and their health benefits:Antihypertensive,antioxidant,and antidiabetic properties[J].Journal of Food Science,2018,83(1):6-16. [23] 曹亚兰.大豆抗氧化肽的制备、分离纯化及结构鉴定[D].广州:华南理工大学,2012. CAO Y L.Study on the preparation,purification and identification of soybean antioxidant peptide[D].Guangzhou:South China University of Technology,2012. [24] 王俊杰.胚胎蛋源性抗氧化肽的制备、分离纯化与结构鉴定[D].南昌:南昌大学,2014. WANG J J.Study on separation,purification and identification of embryonated eggs antioxidant peptide[D].Nanchang:Nanchang University,2014. [25] LUO X,FEI Y,XU Q,et al.Isolation and identification of antioxidant peptides from tartary buckwheat albumin (Fagopyrum tataricum Gaertn.) and their antioxidant activities[J].Journal of Food Science,2020,85(3):611-617. [26] XIA J A,SONG H,HUANG K,et al.Purification and characterization of antioxidant peptides from enzymatic hydrolysate of mungbean protein[J].Journal of Food Science,2020,85(6):1 735-1 741. [27] WANG M,LI C,LI H,et al. In vitro and in silico antioxidant activity of novel peptides prepared from Paeonia ostii ‘Feng Dan’ hydrolysate[J].Antioxidants,2019,8(10):433. [28] ZHANG X,CAO D,SUN X,et al.Preparation and identification of antioxidant peptides from protein hydrolysate of marine alga Gracilariopsis lemaneiformis[J].Journal of Applied Phycology,2019,31(4):2 585-2 596. [29] WANG W Y,ZHAO Y Q,ZHAO G X,et al.Antioxidant peptides from collagen hydrolysate of redlip croaker (Pseudosciaena polyactis) scales:Preparation,characterization,and cytoprotective effects on H2O2-damaged HepG2 Cells[J].Marine Drugs,2020,18(3):156. [30] HU X M,WANG Y M,ZHAO Y Q,et al.Antioxidant peptides from the protein hydrolysate of monkfish (Lophius litulon) muscle:Purification,identification,and cytoprotective function on HepG2 cells damage by H2O2[J].Marine Drugs,2020,18(3):153. [31] YANG J,HUANG J,DONG X,et al.Purification and identification of antioxidant peptides from duck plasma proteins[J/OL].Food Chemistry,2020.DOI:10.1016/j.foodchem.2020.126534. [32] WANG J,LU S,LI R,et al.Identification and characterization of antioxidant peptides from Chinese dry-cured mutton ham[J].Journal of the Science of Food and Agriculture,2020,100(3):1 246-1 255. [33] CHAI T T,LAW Y C,WONG F C,et al.Enzyme-assisted discovery of antioxidant peptides from edible marine invertebrates:A review[J].Marine Drugs,2017,15(2):42. [34] NWACHUKWU I D,ALUKO R E.Structural and functional properties of food protein-derived antioxidant peptides[J].Journal of Food Biochemistry,2019,43(1):e12 761. [35] ZHANG J,DU H Y,ZHANG G N,et al.Identification and characterization of novel antioxidant peptides from crucian carp (Carassius auratus) cooking juice released in simulated gastrointestinal digestion by UPLC-MS/MS and in silico analysis[J].Journal of Chromatography B,2020,1 136:121 893. [36] WITT K A,GILLESPIE T J,HUBER J D,et al.Peptide drug modifications to enhance bioavailability and blood-brain barrier permeability[J].Peptides,2001,22(12):2 329-2 343. [37] WANG C,LI B,WANG B,et al.Degradation and antioxidant activities of peptides and zinc-peptide complexes during in vitro gastrointestinal digestion[J].Food Chemistry,2015,173:733-740. [38] CHEN X,FANG F,WANG S.Physicochemical properties and hepatoprotective effects of glycated Snapper fish scale peptides conjugated with xylose via maillard reaction[J/OL].Food and Chemical Toxicology,2020.DOI:10.1016/j.fct.2020.111115. [39] LIN S,LIANG R,XUE P,et al.Antioxidant activity improvement of identified pine nut peptides by pulsed electric field (PEF) and the mechanism exploration[J].LWT-Food Science and Technology,2017,75:366-372. [40] ZHAO X H,SONG J T.Evaluation of antioxidant properties in vitro of plastein-reaction-stressed soybean protein hydrolysate[J].International Journal of Food Properties,2014,17(1):152-162. [41] YE Q,WU X,ZHANG X,et al.Organic selenium derived from chelation of soybean peptide-selenium and its functional properties in vitro and in vivo[J].Food Function,2019,10(8):4 761-4 770. [42] 郭洪辉,陈晖,俞云,等.河豚鱼皮胶原肽螯合锌的组成与急性毒性研究[J].食品与发酵工业,2020,46(23):41-46. GUO H H,CHEN H,YU Y,et al.Study on the composition and acute toxicity of collagen peptide chelated zinc from puffer skin[J].Food and Fermentation Industries,2020,46(23):41-46. [43] WANG J Z,SUN H M,ZHANG C H,et al.Safety assessment of Maillard reaction products of chicken bone hydrolysate using Sprague-Dawley rats[J].Food &Nutrition Research,2016,60:27 827. [44] 阚旭辉,郭红英,谭兴和,等.可食性植物源抗氧化肽的研究进展[J].湖南农业科学,2016(9):111-114. KAN X H,GUO H Y,TAN X H,et al.Research progress on antioxidant peptides from edible plants source[J].Hunan Agricultural Sciences,2016(9):111-114. [45] RANI S,POOJA K,PAL G K.Exploration of rice protein hydrolysates and peptides with special reference to antioxidant potential:Computational derived approaches for bio-activity determination[J].Trends in Food Science &Technology,2018,80:61-70. [46] AGYEI D,TSOPMO A,UDENIGWE C C.Bioinformatics and peptidomics approaches to the discovery and analysis of food-derived bioactive peptides[J].Analytical and Bioanalytical Chemistry,2018,410(15):3 463-3 472. [47] ZHENG Y J,WANG X,ZHUANG Y L,et al.Isolation of novel ACE-inhibitory and antioxidant peptides from quinoa bran albumin assisted with an in silico approach:Characterization,in vivo antihypertension,and molecular docking[J].Molecules,2019,24(24):4 562. [48] SHANG W H,TANG Y,SU S Y,et al.In silico assessment and structural characterization of antioxidant peptides from major yolk protein of sea urchin Strongylocentrotus nudus[J].Food &Function,2018,9(12):6 435-6 443. [49] BECHAUX J,FERRARO V,SAYD T,et al.Workflow towards the generation of bioactive hydrolysates from porcine products by combining in silico and in vitro approaches[J].Food Research International,2020,132:109 123. [50] CARRASCO-CASTILLA J,HERNÁNDEZ-ÁLVAREZ A J,JIMÉNEZ-MARTÍNEZ C,et al.Use of proteomics and peptidomics methods in food bioactive peptide science and engineering[J].Food Engineering Reviews,2012,4:224-243. [51] AGYEI D,ONGKUDON C M,WEI C Y,et al.Bioprocess challenges to the isolation and purification of bioactive peptides[J].Food and Bioproducts Processing,2016,98:244-256.