A rapid detection method to indicate the age of Shanxi mature vinegarbased on raman spectroscopy
WEN Danhua1,2, LI Yeli1,2, SHI Xiaoya1,2, HUANG Dengyu2,3*
1(School of Life Science, Shanxi University, Taiyuan 030006,China) 2(Food and Drug Safety Rapid Inspection Center, Shanxi University, Taiyuan 030006,China) 3(Shanxi Provincial Market Supervision and Administration Bureau, Taiyuan 030006,China)
Abstract: The aim of this study was to establish a rapid detection method to measure the age of Shanxi mature vinegar based on raman spectra. Firstly, the raman characteristic peak of ligustrazine was determined, followed by optimizing the integral time, integral number, smoothing coefficient, detection power, and sample amount of ligustrazine nano-enhanced raman spectroscopy. The results showed that the optimal working condition was as follows: integrated for 20 s for 10 times, the smoothing coefficient was 10, the power was 300 mW, and the sample volume was 20 μL. The concentration of ligustrazine had a good linear relationship (R2=0.9251) with peak intensity from 5 mg/mL to 200 mg/mL. The precision (2.3%-3.6%) and repeatability (1.6%-3.2%) of the method were good, and the method was accurate as its recovery rate was 80.5%-87.2%. In conclusion, this detection method based on raman spectroscopy can meet the requirements of laboratory and on-site rapid detection, and the experimental results can be used for detecting the age of Shanxi mature vinegar.
温丹华,李叶丽,史晓亚,等. 山西老陈醋醋龄拉曼光谱快速检测方法的建立[J]. 食品与发酵工业, 2019, 45(11): 242-247.
WEN Danhua,LI Yeli,SHI Xiaoya,et al. A rapid detection method to indicate the age of Shanxi mature vinegarbased on raman spectroscopy[J]. Food and Fermentation Industries, 2019, 45(11): 242-247.