以玉米皮为唯一碳源培养裂褶菌，分别测定发酵液中半纤维素酶活性，结果显示木聚糖酶酶活为7.45 U/mL、乙酰木聚糖酯酶酶活为3.41 U/mL、α-L-阿拉伯呋喃糖苷酶和α-葡萄糖醛酸苷酶活相对较低，分别为0.44 U/mL和0.074 U/mL。根据酶活测定结果克隆了裂褶菌的木聚糖酶基因Xyn22，完成了该基因在大肠杆菌中的表达、重组蛋白纯化和性质研究。Xyn22的最适反应温度为40 ℃，当反应温度在45 ℃时其相对酶活在80%以上。Xyn22的最适pH为5.0，在4.5～6.5的范围内酶活均在80%左右。Xyn22在pH 3.0～10.5范围内较稳定，酶活保持在80%左右。Mg2+、Ba2+、EDTA和Hg2+对酶活有很大的抑制作用，Hg2+可以使Xyn22几乎完全失活。

Corn bran is a by-product of corn starch processing.Most of it has not been fully utilized.Microbial fermentation and enzymolysis technology provide new ideas for the utilization of corn bran.Corn bran as the sole carbon source culture of Schizophyllum commune, hemicellulase activity in fermentation broth was determined and the results showed the highest xylanase activity was 7.45 U/mL, acetyl xylan esterase activity was 3.41 U/mL.α-L-arabinofuranosidase and α-glucuronidase activity was relatively low as 0.44 U/mL and 0.074 U/mL.The xylanase gene Xyn22 was cloned according to enzyme activity and its expression in E.coli BL21(DE3).Then purification and characterization of recombinant protein were completed.The optimum reaction temperature was 40 ℃, and the relative enzyme activity was high than 80% when the reaction temperature reached 45 ℃.The optimal pH was 5.0.The activity was about 80% in the range of 4.5- 6.5.Xyn22 was stable in the range of pH 3.0 to 10.5.Mg2+, Ba2+, EDTA and Hg2+ has significant inhibitory effect on the enzyme activity, Hg2+ could almost completely inactivated Xyn22.