采用胰蛋白酶对罗非鱼肉进行酶解，研究酶解产物对Ca2+、Fe2+、Zn2+的结合活性，比较了酶解产物与矿物离子结合后的抗氧化活性。结果表明：水解度为7.41%的酶解产物具有最高的矿物离子结合能力，Ca2+的结合率为78.71%，Fe2+的结合率为73.84%，Zn2+的结合率为71.09%；同时，结合物显示了一定的抗氧化活性，Ca2+结合物1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除率为77.35%，羟自由基清除率为 32.51%，还原力为0.29，均显著高于Fe2+、Zn2+结合物(p<0.05)；氨基酸组成成分分析结果表明：具有高矿物离子结合活性的罗非鱼酶解产物中谷氨酸、天冬氨酸、赖氨酸、丝氨酸含量较高。

The enzymatic hydrolysis from Tilapia meat treated by trypsin, mineral ion binding activities and the antioxidant activities were investigated.The results showed that the hydrolyzates (DH=7.41) had the highest mineral ion binding activity.Its Ca2+ combination rate was 78.71%, Fe2+ combination rate was 73.84%, and Zn2+ combination rate was 71.09%.The combinations exhibited some antioxidant capacity.The Ca2+ combinations scavenging rate of DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and hydroxyl radical, as well as reducing power were 77.35%,32.51%,0.29, respectively, which were significantly higher than those of Fe2+, Zn2+ combinations.In addition, the amino acid sequence analysis showed that the hydrolyzates with higher contents of Glu,Asp,Lys and Ser had the higher mineral ion binding activity.