食品与发酵工业 >

2019 , Vol. 45 >Issue 1: 121 - 127

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.017077

研究报告

萝卜硫素-玉米醇溶蛋白纳米水分散体制备及性质

  • 田倚凡 ,
  • 陈芳 ,
  • 谭小路 ,
  • 赵国华 ,
  • 叶发银
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  • 1(西南大学 食品科学学院,重庆,400715)
    2(食品科学与工程国家级实验教学示范中心(西南大学),重庆,400715)
    3(重庆工商大学 环境与资源学院,重庆,400067)
本科生(叶发银副教授为通讯作者,E-mail:fye@swu.edu.cn)。

收稿日期: 2018-02-12

  网络出版日期: 2019-02-01

基金资助

国家自然科学基金青年科学基金项目 (31601401);重庆市基础科学与前沿技术研究专项一般项目(cstc2017 jcyjAX0430);重庆市教委科学技术研究项目(KJ1706170)

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Preparation and physicochemical properties of sulforaphane- encapsulated zein aqueous nano-dispersions

  • TIAN Yifan ,
  • CHEN Fang ,
  • TAN Xiaolu ,
  • ZHAO Guohua ,
  • YE Fayin
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  • 1(College of Food Science, Southwest University, Chongqing 400715, China)
    2(National Demonstration Center for Experimental Food Science and Technology Education (Southwest University), Chongqing 400715, China)
    3(College of Environment and Resources, Chongqing Technology and Business University, Chongqing 400067, China)

Received date: 2018-02-12

  Online published: 2019-02-01

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摘要

以低浓度的阿拉伯胶为稳定剂,采用反溶剂法制备萝卜硫素-玉米醇溶蛋白纳米水分散体,并对其理化性质及萝卜硫素的释放特性进行研究。结果表明:未包埋萝卜硫素的玉米醇溶蛋白-阿拉伯胶纳米颗粒平均粒径184.1 nm,多分散指数0.135,ζ-电位-33.5 mV,包埋萝卜硫素的颗粒平均粒径159.8~180.2 nm,多分散指数0.152~0.198,ζ-电位-32.1~-37.5 mV,颗粒近球形;当萝卜硫素与玉米醇溶蛋白的质量比为1∶25时,包封率为92.3%,载量43.4 mg/g;随着二者质量比增加,包封率显著下降,载量增幅不大,约60.6~78.7 mg/g。随着pH升高,pH 2~4时颗粒平均粒径显著减小,pH 4~8时其变化不显著;随着环境温度升高,25~55 ℃时,颗粒平均粒径变化不显著,55~85 ℃,平均粒径从200 nm左右增加到350 nm左右。萝卜硫素释放结果表明,60 min内累计释放率接近50%,240 min内几乎完全释放。

关键词： 萝卜硫素; 玉米醇溶蛋白; 纳米颗粒; 阿拉伯胶; 包埋

本文引用格式

田倚凡 , 陈芳 , 谭小路 , 赵国华 , 叶发银 . 萝卜硫素-玉米醇溶蛋白纳米水分散体制备及性质[J]. 食品与发酵工业, 2019 , 45(1) : 121 -127 . DOI: 10.13995/j.cnki.11-1802/ts.017077

Abstract

In this study, the sulforaphane-encapsulated zein aqueous nano-dispersions were prepared by anti-solvent method by using low concentrate gum arabic as a stabilizer. The physicochemical properties and releasing characteristics of sulforaphane were investigated. The results showed that the average size, polydispersity index (PDI), and zeta potential of zein/gum arabic nanoparticles without encapsulating sulforaphane were 184.1 nm, 0.135 and -33.5 mV, respectively. The size, PDI, and zeta potential of sulforaphane-encapsulated zein/gum arabic nanoparticles varied from 159.8 nm to 180.2 nm, 0.152 to 0.198, and -32.1 mV to -37.5 mV, respectively. The TEM results showed that the nanoparticles were nearly spherical. When the weight ratio of sulforaphane to zein was 1∶25, the encapsulation efficiency and loading capacity were 92.3% and 43.4 mg/g, respectively. As the weight ratio increased, the encapsulation efficiency decreased significantly while loading capacity slightly increased in the range of 60.6-78.7 mg/g. When the pH was increasing, the particle sizes of the nanoparticles at pH=2-4 decreased significantly, while changes at pH 4-8 were insignificant. The particle size changed insignificantly at 25-55 ℃, while it increased from 200 nm to about 350 nm with an increase in ambient temperature (55-85 ℃). The results of released sulforaphane showed that the cumulative releasing rate within 60 min was nearly 50% and almost completely released within 240 min. This study provides an experimental reference for encapsulating of sulforaphane.

Key words： sulforaphane; zein; nanoparticles; gum arabic; encapsulation

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