食品与发酵工业 >

2019 , Vol. 45 >Issue 1: 202 - 209

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.016598

分析与检测

顶空固相微萃取-气相色谱串联质谱结合保留指数分析杭白菊挥发性成分

  • 张鹏云 ,
  • 李蓉 ,
  • 李浩洋 ,
  • 龙春霞 ,
  • 杨璐齐 ,
  • 张峰
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  • 1(广东药科大学 公共卫生学院,广东 中山,528458)
    2(中山出入境检验检疫局 检验检疫技术中心,广东 中山,528400)
    3(中国检验检疫科学研究院 食品安全研究所,北京,100176)
硕士研究生(李蓉研究员和龙春霞副教授为共同通讯作者,E-mail:lirong828@126.com,cx-long@163.com)。

收稿日期: 2017-12-25

  网络出版日期: 2019-02-01

基金资助

国家重点研发计划项目“进出口药食同源产品质量检测技术研究”(2017YFF0211000)

收起

Analysis of volatile compounds in Chrysanthemum moriflium Ramat by HS-SPME-GC-MS/MS combing retention index

  • ZHANG Pengyun ,
  • LI Rong ,
  • LI Haoyang ,
  • LONG Chunxia ,
  • YANG Luqi ,
  • ZHANG Feng
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  • 1(School of Public Health,Guangdong Pharmaceutical University,Zhongshan 528458, China)
    2(Inspection and Quarantine Technology Center,Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528400, China)
    3(Institute of Food Safety,Chinese Academy of Inspection and Quarantine,Beijing 100176, China)

Received date: 2017-12-25

  Online published: 2019-02-01

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摘要

采用顶空固相微萃取(headspace solid phase microextraction,HS-SPME)技术,利用气相色谱-三重四极杆串联质谱和自动质谱退卷积定性系统(AMDIS),结合保留指数法分析杭白菊挥发性成分。以峰面积和出峰数为指标,优化得到HS-SPME的较佳萃取条件为:0.3 g杭白菊碎片、50/30 μm二乙基苯/碳分子筛/聚二甲基硅氧烷萃取头、添加1.0 g NaCl并加入8 mL蒸馏水,萃取温度70 ℃、萃取时间50 min、解吸5 min,在较佳萃取条件下检测杭白菊中的挥发性成分。结果表明:从杭白菊中共检测出88种化合物,主要包括烯烃类、醇类、酮类、醛类、酯类、酚类、酸类、烷烃类、芳香烃类等物质,其中相对含量较高的有烯烃类(26.48%)、醇类(23.55%)和酮类(5.86%)。

关键词： 顶空固相微萃取(HS-SPME); 杭白菊; 挥发性成分; 保留指数

本文引用格式

张鹏云 , 李蓉 , 李浩洋 , 龙春霞 , 杨璐齐 , 张峰 . 顶空固相微萃取-气相色谱串联质谱结合保留指数分析杭白菊挥发性成分[J]. 食品与发酵工业, 2019 , 45(1) : 202 -209 . DOI: 10.13995/j.cnki.11-1802/ts.016598

Abstract

Volatiles in Chrysanthemum moriflium Ramat were investigated by using headspace solid-phase microextraction (HS-SPME), gas chromatography-triple quadrupole mass spectrometry(GC-MS/MS), and automated mass spectral deconvolut ion and identification system (AMDIS) based on the retention index (RI). According to the peak area and the number of peaks, the optimal HS-SPME conditions were as follows: extracted 0.3 g Chrysanthemum moriflium Ramat with 1.0 g NaCl and 8 mL distilled water at 70 ℃ for 50 min by the 50/30 μm divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber, then desorb the fibre for 5 min. Under this condition, 88 volatile compounds in total were identified, which were mainly alkenes, alcohols, ketones, aldehydes, esters, phenols, acids, alkanes, and aromatic hydrocarbons. Among them, alkenes (26.48%), alcohols (23.55%), and ketones(5.86%) were predominantly present.

Key words： headspace solid phase microextraction (HS-SPME); Chrysanthemum moriflium Ramat; volatile compounds; retention index

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