综述与专题评论

酶法合成环肽研究进展

  • 程孝中 ,
  • 周志昉 ,
  • 吴志猛
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  • (江南大学,糖化学与生物技术教育部重点实验室,江苏 无锡,214122)
博士(吴志猛教授为通讯作者,E-mail:zwu@jiangnan.edu.cn)。

收稿日期: 2018-07-12

  网络出版日期: 2019-02-01

基金资助

国家自然科学基金项目(21472070);江苏省特聘教授(2014);江苏省“六大人才高峰”( 2014-SWYY-017);江苏省创新团队(2014)

Research progress on enzyme-mediated cyclization of peptides

  • CHENG Xiaozhong ,
  • ZHOU Zhifang ,
  • WU Zhimeng
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  • (Key Laboratory of Carbohydrate Chemistry & Biotechnology, Jiangnan University, Wuxi 214122, China)

Received date: 2018-07-12

  Online published: 2019-02-01

摘要

环肽具有热稳定性、化学稳定性和选择性好,与靶分子亲和力强等优点,可以用于结构活性研究和药物开发。为了更深入研究它们的结构与功能,环肽的制备成为一个重要问题。目前,主要的合成方法为化学合成,这种方法需要全保护的线性肽底物,给合成带来了一定困难。近年来,酶法合成环肽发展为一种重要的合成策略,具有强大的环化能力以及高度的选择性,可以合成更为复杂的环肽。该文就最近几年报道的酶法合成环肽进行综述。

关键词: ; 环肽; 环化; 多肽

本文引用格式

程孝中 , 周志昉 , 吴志猛 . 酶法合成环肽研究进展[J]. 食品与发酵工业, 2019 , 45(1) : 229 -235 . DOI: 10.13995/j.cnki.11-1802/ts.018264

Abstract

Cyclic peptides are thermally and chemically stable. They have good selectivity and enhanced binding affinity towards target biomolecules. They can be used for structure-activity studies and por drug development. In order to further study their structures and functions, preparing cyclic peptides has become an important problem. Up to date, manufacturing cyclic peptides mainly relies on chemical synthesis, which requires fully protected linear peptide substrate and therefore represents a major challenge. Enzyme-mediated cyclization has been developed as a promising strategy due to their extremely powerful ligating ability and excellent selectivity, which can be applied to generate complex cyclic peptides. Herein, recent advances in enzyme-mediated cyclization of peptides were discussed in this review.

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