研究报告

豌豆低聚肽硒螯合物制备及结构特征研究

  • 秦修远 ,
  • 刘文颖 ,
  • 陈立城 ,
  • 刘戚 ,
  • 鲁军 ,
  • 谷瑞增 ,
  • 蔡木易 ,
  • 张春乐
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  • 1(中国食品发酵工业研究院有限公司,北京市蛋白功能肽工程技术研究中心,北京,100015)
    2(广东中食营科生物科技有限公司,广东 东莞,523122)
秦修远助理工程师和刘文颖工程师并列第一作者(蔡木易教授级高工和张春乐工程师为通讯作者,E-mail:caimuyi@vip.sina.com;chunle.zhang@gdsnf.com)。

收稿日期: 2017-09-14

  网络出版日期: 2019-03-11

基金资助

十三五国家重点研发计划(2016YFD0400601);北京市科委专项(北京市科委科技创新基地培育与发展工程专项)(Z161100005016030)

Preparation and structure characterization of selenium-chelatingpea oligopeptides

  • QIN Xiuyuan ,
  • LIU Wenying ,
  • CHEN Licheng ,
  • LIU Qi ,
  • LU Jun ,
  • GU Ruizeng ,
  • CAI Muyi ,
  • ZHANG Chunle
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  • 1 (Beijing Engineering Research Center of Protein and Functional Peptides,China National Research Institute of Food andFermentation Industries,Beijing 100015,China)
    2 (Guangdong Sino Nutri-food Biological Technology Co., Ltd, Dongguan 523122, China)

Received date: 2017-09-14

  Online published: 2019-03-11

摘要

以豌豆低聚肽和亚硒酸钠为原料,采用正交法研究了制备豌豆低聚肽硒螯合物的最佳工艺,结果表明,在温度为80 ℃,豌豆低聚肽质量浓度为50 g/L,肽盐质量比为2∶1,pH=9时,螯合30 min,产物得率为27.87%,螯合率为57.23%。通过紫外全波长扫描、扫描电镜、傅里叶红外光谱分析豌豆低聚肽螯合前后结构特征变化,定性结果表明,螯合前后物质的结构发生了变化,对光吸收性能加强,硒离子与低聚肽中NH2+以及—COO—形成配位键。

本文引用格式

秦修远 , 刘文颖 , 陈立城 , 刘戚 , 鲁军 , 谷瑞增 , 蔡木易 , 张春乐 . 豌豆低聚肽硒螯合物制备及结构特征研究[J]. 食品与发酵工业, 2019 , 45(3) : 28 -33 . DOI: 10.13995/j.cnki.11-1802/ts.015777

Abstract

Pea oligopeptides and sodium selenite were used as raw materials, the best process for preparing selenium-chelating pea oligopeptides was studied by using orthogonal methods. The results showed that when chelating at 80 ℃ for 30 min, with 5% pea oligopeptides and an oligopeptide to salt mass ratio 2∶1 and at pH=9, the yield and the chelating rate were 27.87% and 57.23%, respectively. The structural characteristics of pea oligopeptides were analyzed by ultraviolet full wavelength scanning (UV), scanning electron microscopy, and infrared radiation (IR). The results showed that the structure of the chelating substances changed before and after chelation, and the optical absorption properties were enhanced. The coordination bonds between selenium ions and NH2+ and —COO— in oligopeptides were formed.

参考文献

[1] 吴建中,赵谋明,宁正祥. 食品中的生物活性多肽[J]. 食品与发酵工业, 2002, 28(11):46-50.
[2] 张秋萍. 豌豆分离蛋白酶解产生物活性肽的研究[D]. 无锡:江南大学, 2013.
[3] 范小飞,虞建宏. 微量元素硒的生物学功能及测定方法的研究[J].江苏预防医学,2011,22(1): 62-63.
[4] 莫莹,李亚男,王建,等.藻类硒蛋白研究进展[J]. 安徽农业科学,2013,41(17):7 399-7 403.
[5] 唐新欣,贺蓉. 中国缺硒状况的调查[J]. 慢性病学杂志, 2002(6):22-24.
[6] WANG Y D, WANG X, WONG Y S. Generation of selenium-enriched rice with enhanced grain yield, selenium content and bioavailability through fertilisation with selenite[J]. Food Chemistry, 2013, 141(3):2 385.
[7] C-B′HYMER C, CARUSO J A. Selenium speciation analysis using inductively coupled plasma-mass spectrometry[J]. Journal of Chromatography A, 2006, 1114(1):1-20.
[8] 代向向. 云芝生物转化无机硒生产富硒云芝糖肽的研究[D]. 上海:上海师范大学, 2015.
[9] 李洪艳. 真鲷鱼头制取鱼油及肽硒螯合物的研究[D]. 天津:天津科技大学, 2013.
[10] 贾姗姗,朱连勤,朱风华,等. 3,3′-二氨基联苯胺分光光度法检测硒[J].畜牧与兽医, 2012,44(6):74-76.
[11] 高菲,王维有,鲁军,等. 海洋鱼骨胶原肽钙螯合物的制备及红外光谱表征[J]. 中国海洋大学学报(自然科学版), 2015, 45(1):47-54.
[12] 宋莎莎,高菲,任迪峰,等. 乌鸡肽铁(Ⅱ)螯合物的制备及红外光谱鉴定[J]. 食品与发酵工业, 2013, 39(6):13-17.
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