复水是冷冻干燥食品的重要特性,为明确冷冻干燥豆腐复水后其水分分布的规律,采用低场核磁共振研究了不同超声功率(0、100、200、300、400,500 W)制浆对鲜、复水冷冻干燥豆腐水分分布及微观结构的影响。豆腐持水性测定结果表明:复水冷冻干燥豆腐的持水性显著低于鲜豆腐,这可能是由于冷冻干燥处理使复水豆腐的微观结构发生变化,孔洞的尺寸变大,进而降低对水分子的保留率。水分分布测定结果表明:适宜的超声处理可提供鲜、复水冷冻干燥豆腐更多可用于截流自由水组分的结构,缩短T21弛豫时间,增大M21峰面积百分数。扫描电子显微镜观测结果表明:超声制浆及冷冻干燥工艺均可显著影响两种豆腐的微观结构。
Rehydration is an important characteristic of freeze-dried foods, to clarify the rule of moisture distribution of rehydrated freeze-dried tofu, effects of ultrasound power (0, 100, 200, 300, 400, 500 W) on moisture distribution and microstructure of fresh tofu and rehydrated freeze-dried tofu were studied by low field nuclear magnetic resonance (NMR). The results showed that the water holding capacity of freeze-dried tofu was significantly lower than that of fresh tofu, which may be due to the changes in the size of the hole in the microstructure of rehydrated freeze-dried tofu, and therefore reduced the retention of water molecules. The results of water distribution showed that suitable ultrasound treatment could provide fresh tofu and rehydrated freeze-dried tofu more structures for intercepting free water components, decreased the T21 relaxation time and increased the M21 peak area percentage. The results of scanning electron microscope showed that ultrasound pulping and freeze drying process could significantly influence the microstructures of these two kinds of tofu.
[1] 庞中伟,张泽俊. 冷冻豆腐和冷冻干燥豆腐加工[J]. 食品科学, 2004, 25(S1): 128-130.
[2] HU H, CHEUNG I W Y, PAN S, et al. Effect of high intensity ultrasound on physicochemical and functional properties of aggregated soybean β-conglycinin and glycinin[J]. Food Hydrocolloids, 2015, 45: 102-110.
[3] O′SULLIVAN J, PARK M, BEEVERS J. The effect of ultrasound upon the physicochemical and emulsifying properties of wheat and soy protein isolates[J]. Journal of Cereal Science, 2016, 69: 77-84.
[4] HU H, FAN X, ZHOU Z, et al. Acid-induced gelation behavior of soybean protein isolate with high intensity ultrasonic pre-treatments[J]. Ultrasonics Sonochemistry, 2013, 20(1): 187-195.
[5] LIN H F, LU C P, HSIEH J F, et al. Effect of ultrasonic treatment on the rheological property and microstructure of tofu made from different soybean cultivars[J]. Innovative Food Science & Emerging Technologies, 2016, 37: 98-105.
[6] ZHANG Peipei, HU Tan, FENG Shaolong, et al. Effect of high intensity ultrasound on transglutaminase-catalyzed soy protein isolate cold set gel[J]. Ultrasonics Sonochemistry, 2016,29:380-387.
[7] 齐宝坤,李杨,王中江.超声处理对豆腐品质的影响及工艺优化[J]. 食品工业, 2016,37(2):138-141.
[8] HARNKARNSUJARIT N, KAWAI K, WATANABE M, et al. Effects of freezing on microstructure and rehydration properties of freeze-dried soybean curd[J]. Journal of Food Engineering, 2016, 18(4):10-20.
[9] BERTRAM H C, ERSEN H J. Applications of NMR in meat science[J]. Annual Reports on Nmr Spectroscopy, 2004, 53(4): 157-202.
[10] LI T, RUI X, WANG K, et al. Study of the dynamic states of water and effects of high-pressure homogenization on water distribution in tofu by using low-field nuclear magnetic resonance[J]. Innovative Food Science & Emerging Technologies, 2015, 30(1): 61-68.
[11] LI T, RUI X, LI W, et al. Water distribution in tofu and application of T2 relaxation measurements in determination of tofu’s water-holding capacity[J]. Journal of Agricultural & Food Chemistry, 2014, 62(34): 8 594-8 601.
[12] 朱巧梅,郦金龙,刘妍,等. 新型W/O盐卤凝固剂对大豆蛋白凝胶中水分变化的影响[J]. 中国粮油学报, 2014, 29(5): 100-105.
[13] LI Yong,SHI Whentao, CHENG Shasha, et al. Freezing-induced proton dynamics in tofu evaluated by low-field nuclear magnetic resonance[J]. Journal of Food Measurement & Characterization, 2017,11(3):1 003-1 010.
[14] AND M C P, AÑÓN M C. Structural properties of heat-induced soy protein gels as affected by ionic strength and pH[J]. Journal of Agricultural & Food Chemistry, 1998, 46(9): 3 583-3 589.
[15] 胡昊. 利用高场强超声波增强大豆蛋白凝胶性及凝胶缓释效果[D]. 武汉:华中农业大学, 2014: 43-47.
[16] WU W, HUA Y, LIN Q, et al. Effects of oxidative modification on thermal aggregation and gel properties of soy protein by peroxyl radicals[J]. International Journal of Food Science & Technology, 2011, 46(9): 1 891-1 897.
[17] KAO F J, NANWEI SU A, LEE M H. Effect of calcium sulfate concentration in soymilk on the microstructure of firm tofu and the protein constitutions in tofu whey[J]. Journal of Agricultural & Food Chemistry, 2003, 51(21): 6 211-6 216.
[18] JR I D K, KAUZMANN W. Hydration of proteins and polypeptides[J]. Advances in Protein Chemistry, 1974, 28(1): 239-345.
[19] YASUI T, ISHIOROSHI M, NAKANO H, et al. Changes in shear modulus, ultrastructure and spin-spin relaxation times of water associated with heat-induced gelation of myosin[J]. Journal of Food Science, 1979, 44(4): 1 201-1 204.
[20] 韩敏义,刘永安,王鹏,等. 低场核磁共振法研究NaCl对肌原纤维蛋白凝胶水分分布和移动性的影响[J]. 食品科学, 2014, 35(21): 88-93.
[21] 李腾. 低场核磁共振研究大豆和豆腐中的水分分布[D]. 南京:南京农业大学, 2015: 31-45, 52-57.
[22] 杨慧娟,韩敏义,邹玉峰,等. 低场核磁共振研究高压处理对乳化肠特性的影响[J]. 食品科学, 2014, 35(17): 53-57.
[23] 李汴生,阮征,曾庆孝. 超高压处理对豆浆凝胶特性的影响[J]. 食品与发酵工业, 1999, 24(1): 12-17.
[24] 辛颖. 低频超声波辅助提高西兰花冻结与冻藏品质及效率的研究[D]. 无锡:江南大学, 2014: 24-25.
[25] 徐保国. 低频超声波对红心萝卜的冻结和解冻及其机理研究[D]. 无锡:江南大学, 2016: 60-62.
[26] 张慜,李春丽. 生鲜食品新型加工及保藏技术[M]. 北京:中国纺织出版社, 2011.
[27] MALTAIS A, REMONDETTO G E, SUBIRADE M. Mechanisms involved in the formation and structure of soya protein cold-set gels: A molecular and supramolecular investigation[J]. Food Hydrocolloids, 2008, 22(4): 550-559.
[28] HU H, ECY L C, WAN L, et al. The effect of high intensity ultrasonic pre-treatment on the properties of soybean protein isolate gel induced by calcium sulfate[J]. Food Hydrocolloids, 2013, 32(2): 303-311.
[29] RENKEMA J M S, KNABBEN J H M, VLIET T V. Gel formation by β-conglycinin and glycinin and their mixtures[J]. Food Hydrocolloids, 2001, 15(4-6): 407-414.