试验以黄鹤楼酒浓香型窖泥为材料,采用传统微生物分离方法即稀释涂布平板法对样品进行涂布,然后平板划线纯化、镜检,单菌发酵利用GC-MS筛选出高产愈创木酚类物质的功能菌株,然后利用分子生物学技术对菌种进行鉴定,最后进行菌种的耐受性分析。试验结果为:筛选出2株高产愈创木酚类物质的功能菌B-1、B-3,经鉴定分别为Bacillus amyloliquefaciens、Delftia sp.X-a12,总产量分别为3 632.33和14 527.31 μg/L。耐受性分析结果为菌株B-1、B-3均耐4%vol酒精度、pH 4.5、15%糖度。
The experiment was carried out using dilution coating method to coat the mud of the Yellow Crane Tower Luzhou-flavor liquor. Plate scribing purification, microscopic examination, and single-bacteria fermentation were conducted, followed by using gas chromatography-mass spectrometer (GC-MS) to screen functional bacteria with high yield of guaiacol. The results of molecular biology techniques showed that two functional strains, B-1 and B-3, with high-yield of guaiacol were Bacillus amyloliquefaciens and Delfitia sp. X-a12, respectively. Their total yields were 3 632.33 μg/L and 14 527.31 μg/L, respectively. The tolerance analysis results showed that both strains were resistant to 4% vol. alcohol, pH=4.5, and 15% sugar.
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