为探究pH变换法对鱼蛋白气味特性的影响,以蓝点马鲛鱼为研究对象,在生熟两种状态下,对比原料肉、传统漂洗鱼糜、酸碱鱼分离蛋白的一般化学组成及氨基酸组成,重点探讨酸碱鱼分离蛋白的气味特性。利用电子鼻技术定性判断4种样品的气味轮廓差异,进一步利用固相微萃取气相色谱和质谱联用(solid phase microextraction-gas chromatography mass spectrometry,SPME-GC-MS)对挥发性物质进行定量分析。结果显示,电子鼻可以有效区分4种熟制样品的气味轮廓,与原料肉相比,气味轮廓差异按照由大到小的顺序为:漂洗鱼糜>酸分离蛋白>碱分离蛋白,生制样品的差异性顺序与熟制相同但是区分度较小;进一步经GC-MS定量分析,在4种生样中分别检测出 26、19、23、22种挥发性成分,在4种熟样中分别检出 23、26、32、24种挥发性成分,均以醛、酮、醇类为主。与原料肉相比,其他3种经过处理的样品中醇、酮类挥发性物质种类及含量都发生改变,且熟制样品与生制样品间含量差异较大。由此说明pH变换法能够有效降低蓝点马鲛鱼的腥味,得到的鱼分离蛋白与漂洗鱼糜相比具有较好的气味特性。因此,对腥味较重的蛋白源可利用此方法回收得到具有良好气味、蛋白含量高、脂质含量低等特点的蛋白,并为其深加工提供理论依据。
This study aimed to explore the effect of pH shift on odor characteristics of fish protein. Scomberomorus niphonius was used as the research object in raw and cooked states. The proximate composition and amino acid composition of raw meat, water washing surimi (WM), acid-aided protein isolates samples(AC), and alkali-aided protein isolates samples(AK) were compared. The odor characteristics of AC and AK were focused. Electronic nose (E-nose) was used to determine the differences in odor profiles of the four samples. The odor substances were further quantified by solid phase microextraction-gas chromatography mass spectrometry (SPME-GC-MS). The results showed that E-nose could effectively distinguish the odor profiles of the four cooked samples. Compared to raw meat, the differences in odor profiles from the largest to the smallest: WM>AC>AK. Compared with cooked samples, raw samples had the same variability but less discrimination. Further quantitative analysis showed that twenty-six, nineteen, twenty-three, and twenty-two volatile compounds were detected in four raw samples. Twenty-three, twenty-six, thirty-two, and twenty-four volatile compounds were detected in four cooked samples. Aldehydes, ketones, and alcohols were the main volatile compounds in all samples. Compared with raw meat, the kinds and contents of alcohols and ketones in other processed samples changed, and there was a big difference between cooked and raw samples. It was concluded that pH shift could effectively reduce the fishy off-flavor of Scomberomorus niphonius. The fish protein isolates had better odor characteristics than WM. Therefore, protein sources with heavy fishy off-flavor could use this method to make proteins have good odor and have high protein content and low lipid content etc., providing a theoretical basis for its further processing.
[1] 谢婷婷. 冻藏鲅鱼品质变化的研究[D]. 大连:大连工业大学,2015.
[2] 史策,韩烽烽,刘鹏,等.鳕鱼和鲅鱼鱼肉蛋白酶解产物功能特性及抗氧化性[J]. 肉类研究, 2013,27(8):5-7.
[3] 魏华茂,杨文鸽,张梦芸,等.带鱼鱼糜漂洗水中回收蛋白的性质及其在鱼糜中的再利用[J].现代食品科技,2016,32(6):321-327.
[4] NOLSØE H, UNDELAND I. The acid and alkaline solubilization process for the isolation of muscle proteins: state of the art[J]. Food & Bioprocess Technology, 2009, 2(1):1-27.
[5] 刘诗长. 罗非鱼分离蛋白的制备及其性质研究[D]. 湛江:广东海洋大学,2011.
[6] 袁凯,张龙,谷东陈,等. 基于漂洗工艺探究白鲢鱼糜加工过程中蛋白质氧化规律[J]. 食品与发酵工业,2017,43(12):30-36.
[7] WHITE S S, FOX K M, JERVIS S M, et al. Influence of heating and acidification on the flavor of whey protein isolate[J]. Journal of Dairy Science,2013,96(3):1 366-1 379.
[8] 胡传明,王丹青,王锡昌,等. 条斑紫菜与坛紫菜挥发性气味的电子鼻分析[J]. 广西科学,2016,23(2):138-143.
[9] 崔方超,李婷婷,杨兵,等. 电子鼻结合GC-MS分析草鱼脱腥前后气味变化[J]. 食品科学,2014,35(20):126-130.
[10] 施文正. 草鱼肉挥发成分及其影响因素的研究[D]. 上海:上海海洋大学,2010.
[11] 顾赛麒,陶宁萍,吴娜,等. 一种基于ROAV值鉴别蟹类关键特征性气味物的方法[J]. 食品工业科技,2012,33(13):410-416.
[12] 付庆. 酸碱处理对于鲢鱼鱼糜品质的影响[D]. 无锡:江南大学, 2009.
[13] 王方. 酸碱处理对鲫鱼糜腥味脱除效果及凝胶特性影响的研究[D]. 武汉:华中农业大学,2014.
[14] 常通. 酸碱法提取鳙鱼蛋白及鱼糜凝胶品质的研究[D]. 武汉:华中农业大学, 2014.
[15] FU X, XU S, WANG Z. Kinetics of lipid oxidation and off-odor formation in silver carp mince: The effect of lipoxygenase and hemoglobin[J]. Food Research International, 2009, 42(1): 85-89.
[16] 王建辉,刘永乐,刘冬敏,等. 冷藏期间草鱼鱼片脂肪氧化变化规律研究[J]. 食品科学,2013,34(6):243-246.
[17] 张培培,吴雪燕,汪淼,等.肉制品中脂肪氧化与蛋白质氧化及相互影响[J].食品与发酵工业,2013,39(5):143-148.
[18] PAKER I, BEAMER S, JACZYNSKI J, et al. Compositional characteristics of materials recovered from headed gutted silver carp (Hypophthalmichthys molitrix) by isoelectric solubilization and precipitation using organic acids[J]. Journal of Food Science, 2013, 78(3):E445-E451.
[19] MARMON S K, UNDELAND I. Protein isolation from gutted herring (Clupea harengus) using pH-shift processes.[J]. Journal of Agricultural & Food Chemistry, 2010, 58(19):10 480.
[20] 张强,王倩倩,陆剑锋,等. 不同方法提取鲢鱼皮胶原蛋白的理化特性比较[J]. 现代食品科技,2014,30(5):104-110.
[21] 王帅,吴旭干,陶宁萍,等. 中华绒螯蟹六月黄营养及气味品质研究[J]. 中国食品学报,2017,17(4):219-227.
[22] 高先楚,王锡昌,顾赛麒,等.中华绒螯蟹性腺加热熟制前后挥发性成分和脂肪酸组成分析[J]. 现代食品科技,2014,30(9):265-274.
[23] 韩小孩,张耀辉,孙福军,等. 基于主成分分析的指标权重确定方法[J]. 四川兵工学报,2012,33(10):124-126.
[24] 段秀霞,施文正,汪之和,等. 熟制与贮藏对凡纳滨对虾挥发性成分的影响[J]. 水产学报,2017,41(6):971-983.
[25] GLENDA FRATINI, SALOMÉ LOIS, MANUEL PAZOS,et al. Volatile profile of Atlantic shellfish species by HS-SPME GC/MS[J]. Food Research International,2012,48(2):856-865.
[26] 付湘晋,许时婴,王璋,等. 酸碱处理对鲢鱼肌原纤维蛋白热变性、聚集、胶凝性质的影响[J]. 食品科学,2008,29(6):100-103.
[27] JOSEPHSON D B, LINDSAY R C, STUIBER D A. Identification of compounds characterizing the aroma of fresh whitefish (Coregonus clupeaformis)[J]. Journal of Agricultural and Food Chemistry, 1983,31(2):326-330.
[28] 刘纯友. 不同牛种组织中功能性脂质分析及热处理对脂质氧化影响研究[D]. 武汉:华中农业大学, 2015.
[29] 林婉玲,杨贤庆,侯彩玲,等. 浸渍冻结对凡纳滨对虾冻藏过程中品质的影响[J]. 食品科学,2014,35(10):223-229.
[30] 陈小冬,江猛,王菊琳,等. 白鲢鱼糜熟制后挥发性气味的对比[J]. 食品科技,2016,41(4):152-160.
[31] 杜国伟. 鲢鱼糜的脱腥技术研究[D]. 无锡:江南大学,2007.
[32] SRINIVASAN D, JOHN E. Kinsella. Binding of carbonyls to fish actomyosin[J]. Journal of Agricultural and Food Chemistry, 1983, 31(4):856-859.
[33] SIEK T J, ALBIN I A, SATHER L A,et al. Comparison of flavor thresholds of aliphatic lactones with those of fatty acids, esters, aldehydes,alcohols, and ketones1,2[J]. Journal of Dairy Science,1971, 54(1):1-4.
[34] 田迪英,焦慧,陶崴,等. 5种海鱼挥发性气味成分分析[J]. 食品与发酵工业,2015,41(10):155-159.
[35] 钱琴莲,李晔,王求娟,等. 基于GC-MS和电子鼻技术的金枪鱼胰脏酶解气味解析[J]. 食品科学,2016,37(8):121-126.
[36] 刘安军,褚兰玲,郑婕,等. 鲤鱼土腥味成分的检测与去除[J]. 现代食品科技,2011,27(2):219-223.