异常汉逊酵母(Hansenula anomala)具有较强的发酵力和酯化力,并能积累色氨酸。为了进一步理解其发酵过程中的基因表达与代谢的关系,在转录组测序的基础上,利用生物信息学方法,分别对5个发酵时间点(0、24、48、72、96 h)的异常汉逊酵母菌的差异表达基因的功能进行了分析。结果表明,各发酵时间点与其相邻的上一个时间点相比,发酵24、48、72、96 h的上调表达的基因数量分别为585、487、154、615,下调表达的基因数量分别为1 112、725、5、245。差异表达基因功能的GO富集和KEGG通路富集结果表明,异常汉逊酵母菌的代谢强度在0~48 h下降,48~96 h上升,并且在96 h时,具有较强的代谢活动和遗传信息处理能力。产色氨酸通路的差异基因表达矩阵分析表明,异常汉逊酵母菌合成色氨酸的速率在0~24 h下降,随后上升;而色氨酸的分解速率从0~48 h上升,随后下降。这些结果可为异常汉逊酵母菌的分子育种及其代谢调控研究提供理论依据。
Hansenula anomala has outstanding performances on fermentation, esterification, and accumulating tryptophan. To illustrate the relationship between genes expression and metabolism of H. anomala during fermentation, differentially expressed genes (DEGs) at different fermentation points (0, 24, 48, 72, 96 h) were analyzed by using transcriptomic sequencing and bioinformatic methods. The results showed that 585, 487, 154, and 615 genes were up-regulated at 24, 48, 72, 96 h, respectively, while 1112, 725, 5, and 245 genes were down-regulated. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment of DEGs indicated that the metabolic activity of H. anomala decreased from 0 h to 48 h, but increased from 48 h to 96 h. Additionally, H. anomala had strong metabolic activity and genetic information processing ability at 96 h. The differential gene expression matrix analysis of the tryptophan pathway showed that the synthesis rate of tryptophan in H. anomala decreased from 0 h to 24 h and increased thereafter, while the decomposition rate of tryptophan increased from 0 h to 48 h, and then decreased. In summary, these results provides a theoretical basis for molecular breeding and metabolic regulation of H. anomala.
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