以短波紫外线照射后双孢菇为实验材料,采用高效液相色谱法测定维生素D2(VD2)的含量,利用响应面法优化其VD2的皂化工艺。利用单因素实验确定的醇碱体积比,以液固比、皂化温度、皂化时间为自变量,VD2的提取量为响应值,采用响应面法分析研究各因素对VD2的提取量的影响,确定最佳工艺参数。结果表明,双孢菇中VD2的最佳皂化工艺为醇碱体积比2∶1,液固比12∶1(mL∶g),皂化温度75 ℃,皂化时间63 min,此条件下,VD2提取量为8.13 μg/g,与理论值相近。实验建立的皂化工艺切实可行,为食用菌中VD2的开发利用提供理论参考。
This study was conducted to optimize the saponification process of vitamin D2(VD2)in white button mushrooms by response surface methodology after irradiating the mushrooms by UV-C. The VD2 content was measured by high performance liquid chromatography. Volume ratio of alcohol to alkali was determined by single factor experiments. The liquid to solid ratio, saponification temperature, and saponification time were taken as independent variables, and the amount of VD2 extracted was the response value. The effects of various factors on extracting VD2 and the optimum process parameters were determined by the response surface methodology. The results showed that the best saponification process of VD2 in white button mushrooms was as follows: the volume ratio of alcohol to alkali was 2∶1(V∶V), the liquid-solid ratio was 12∶1(mL∶g), the saponification temperature was 75 ℃, and the saponification time was 63 min. Under this condition, the amount of VD2 extracted was 8.13 μg/g, which was similar to the theoretical value. The established saponification process is practical, and it provides a theoretical reference for the development and utilization of VD2 in the mushroom.
[1] 中国农业百科全书总编辑委员会蔬菜卷编辑委员会. 中国农业百科全书蔬菜卷[M]. 北京:农业出版社, 1990.
[2] 张金霞,陈强,黄晨阳,等. 食用菌产业发展历史、现状与趋势[J]. 菌物学报, 2015, 34(4): 524-540.
[3] 史琦云,邵威平. 八种食用菌营养成分的测定与分析[J]. 甘肃农业大学学报, 2003(3): 336-339;345.
[4] 吴素玲,孙晓明,王波,等. 双孢蘑菇子实体营养成分分析[J]. 中国野生植物资源, 2006(2): 47-48;52.
[5] SLAWIN′SKA A, FORNAL E, RADZKI W, et al. Study on vitamin D2 stability in dried mushrooms during drying and storage[J]. Food Chemistry, 2016,199: 203-209.
[6] TEICHMANN A, DUTTA P C, STAFFAS A, et al. Sterol and vitamin D2 concentrations in cultivated and wild grown mushrooms:Effects of UV irradiation[J]. LWT - Food Science and Technology, 2007, 40(5): 815-822.
[7] JASINGHE V J, PERERA C O. Ultraviolet irradiation: The generator of Vitamin D-2 in edible mushrooms[J]. Food Chemistry, 2006, 95(4): 638-643.
[8] KOYYALAMUDI S R, JEONG S C, SONG C H, et al. Vitamin D2 formation and bioavailability from agaricus bisporus button mushrooms treated with ultraviolet irradiation[J]. Journal of Agricultural and Food Chemistry, 2009, 57(8): 3 351-3 355.
[9] HUANG S J, LIN C P, TSAI S Y. Vitamin D-2 content and antioxidant properties of fruit body and mycelia of edible mushrooms by UV-B irradiation[J]. Journal of Food Composition and Analysis, 2015(42): 38-45.
[10] 孙梦姣. 紫外照射提高双孢蘑菇中VD2含量的研究[D]. 杭州:浙江大学, 2013.
[11] 李丽,蒋景龙. 紫外光影响食用菌中VD2含量的研究进展[J]. 食品科学, 2015, 36(1): 273-277.
[12] KALARAS M D, BEELMAN R B, ELIAS R J. Effects of postharvest pulsed UV light treatment of white button mushrooms (Agaricus bisporus) on vitamin D2 content and quality attributes[J]. Journal of Agricultural and Food Chemistry, 2012, 60(1): 220-225.
[13] CHEN S Y, YU H T, KAO J P, et al. Consumption of vitamin D2 enhanced mushrooms is associated with improved bone health[J]. Journal of Nutritional Biochemistry, 2015, 26(7): 696-703.
[14] TAOFIQ O, FERNANDES Â, BARROS L, et al. UV-irradiated mushrooms as a source of vitamin D2: A review [J]. Trends in Food Science & Technology, 2017(70): 82-94.
[15] KRINGS U, BERGER R G. Dynamics of sterols and fatty acids during UV-B treatment of oyster mushroom[J]. Food Chemistry, 2014, (149): 10-14.
[16] PHILLIPS K M, RUGGIO D M, HORST R L, et al. Vitamin D and sterol composition of 10 types of mushrooms from retail suppliers in the united states[J]. Journal of Agricultural and Food Chemistry, 2011, 59(14): 7 841-7 853.
[17] JIANG Tianjia, JAHANGIR M M, JIANG Zhenhui, et al. Influence of UV-C treatment on antioxidant capacity, antioxidant enzyme activity and texture of postharvest shiitake (Lentinus edodes) mushrooms during storage[J]. Postharvest Biology and Technology, 2010, 56(3): 209-215.
[18] NÖLLE N, ARGYROPOULOS D, AMBACHER S, et al. Vitamin D-2 enrichment in mushrooms by natural or artificial UV-light during drying[J]. LWT - Food Science and Technology, 2017(85): 400-404.
[19] 胡彬彬,武忠伟,王振河,等. 紫外辐照对鸡腿菇中VD2含量的影响[J]. 湖北农业科学, 2012, 51(13): 2 758-2 760.
[20] KALARAS M D, BEELMAN R B, HOLICK M F, et al. Generation of potentially bioactive ergosterol-derived products following pulsed ultraviolet light exposure of mushrooms (Agaricus bisporus)[J]. Food Chemistry, 2012, 135(2): 396-401.
[21] HUANG S J, LIN C P, TSAI S Y. Vitamin D2 content and antioxidant properties of fruit body and mycelia of edible mushrooms by UV-B irradiation[J]. Journal of Food Composition and Analysis, 2015, 42: 38-45.
[22] 严丽娟,李文斌,洪煜琛,等. 液相色谱-大气压化学电离串联质谱法测定婴幼儿配方奶粉中的VD[J]. 色谱, 2017, 35(4): 427-431.
[23] 姜雨. 番茄中番茄红素提取及稳定性研究[D]. 北京:中国农业大学, 2004.
[24] 李德和,王君为,赵越. 响应面法优化类球红细菌中番茄红素提取工艺[J]. 食品科学, 2015, 36(10): 16-22.
[25] 徐向宏,何明珠. 试验设计与Design-Expert、SPSS应用[M]. 北京:科学出版社, 2010.
[26] 李莉,张赛,何强,等.响应面法在试验设计与优化中的应用[J]. 实验室研究与探索, 2015, 34(8): 41-45.
[27] PLUDOWSKI P, HOLICK M F, GRANT W B, et al. Vitamin D supplementation guidelines[J]. Journal of Steroid Biochemistry & Molecular Biology, 2018, 175: 125-135.