基于微流控技术与分光光度法,研制了一套用于水产品中甲醛、双氧水和SO2快速检测的微流控芯片系统。该系统在一次性扇形微流控芯片上集成了进样、显色反应及检测单元,每张芯片可同时检测甲醛、双氧水和SO2三种指标。结果显示,微流控芯片系统能够在5 min内实现对水产品中甲醛、双氧水、SO2三种指标的准确检测,检出限分别可达到0.3、0.4、0.2 mg/L,回收率在92.38%~107.98%,相对标准偏差均低于4%。微流控芯片系统能够实现对水产品中甲醛、双氧水和SO2进行现场、快速、全自动、高通量检测,适合基层非专业人员开展筛选需求,体现了微流控芯片在食品快速检测应用中的巨大潜力。
Based on microfluidic technology and spectrophotometry, a microfluidic chip system for rapid detection of formaldehyde, hydrogen peroxide, and sulfur dioxide in aquatic products was developed. This disposable fan-shaped microfluidic chip was integrated with sampling, color reaction, and detection chamber. Each chip could detect 3 indexes such as formaldehyde, hydrogen peroxide, and sulfur dioxide at the same time. The results showed that the microfluidic chip system could accurately detect formaldehyde, hydrogen peroxide, and sulfur dioxide in aquatic products in 5 minutes with detection limits of 0.3 mg/L, 0.4 mg/L, and 0.2 mg/L, respectively. The recovery rate was 92.38%-107.98%, and the relative standard deviation was less than 4%. The microfluidic chip system can realize on-site, rapid, automatic, and high throughput detection of formaldehyde, hydrogen peroxide and sulfur dioxide in aquatic products, which is suitable for unprofessional individuals to screen the products, showing a great potential of applying microfluidic chip in rapid detection for foods.
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