在pH 3.46 的酸性介质中,卡马西平与考马斯亮蓝-溴代十六烷基吡啶反应生成离子缔合物,使共振光散射(resonance light scattering, RLS)信号明显增强,在最大共振光散射波长344 nm处,卡马西平的质量浓度在0.006～0.33 mg/L与体系共振光散射强度的增加值(ΔIRLS)呈良好的线性关系,检出限为0.005 6 mg/L,定量限为0.012 mg/kg。据此建立了快速、准确、高灵敏测定肉食中残留卡马西平的RLS新方法。该法用于肉食中残留卡马西平含量的测定,回收率为98.5%～102%,相对标准偏差(n=5)为2.2%～2.6%。
In an acidic medium of pH 3.46, carbamazepine reacted with coomassie brilliant blue- cetylpyridyl bromide to form an ion association, which led to the resonance light scattering (RLS) signal increased obviously. At the maximal resonant light scattering wavelength of 344 nm, the mass concentration of carbamazepine in the range of 0.006-0.33 mg/L showed a good linear relationship with the increase in resonant light scattering intensity (ΔIRLS). The detection limit was 0.0056 mg/ L and the limit of quantitation was 0.012 mg/kg. Based on the above results, a rapid, accurate, highly sensitive new RLS method for determining carbamazepine in meat foods was established. This method was applied to determine residual carbamazepine in meat foods with 98.5%-102% recovery rate and the relative standard deviation (n=5) was 2.2%-2.6%.
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