通过改良丁酸梭菌筛选培养基,提高了丁酸梭菌的筛选效率,获得1株高产胞外多糖(exopolysaccharide,EPS)的丁酸梭菌RO-07。进一步研究丁酸梭菌RO-07的肠道耐受性和抗生素敏感性,结果表明,该菌株对质量浓度为0.5~4 g/L的牛胆盐具有良好的耐受性,对人工胃液和人工肠液有较好的耐受性,对氨基糖苷类抗生素的耐药性较强。对丁酸梭菌EPS的抗氧化特性研究表明,终质量浓度为0.33 mg/mL的EPS对·OH的清除活性达到75.2%,对O-2·清除能力达到95%,具有很强的抗氧化性。
The screening efficiency of Clostridium butyricum was improved by optimizing the screening medium. The C. butyricum strain RO-07 with high-yield of exopolysaccharide (EPS) was obtained and identified. The intestinal tolerance and antibiotic susceptibility of C. butyricum RO-07 were further analyzed. The results showed that the strain RO-07 possessed superior tolerance to 0.05%-0.40% bovine bile salts, as well as to simulated gastric juice and intestinal fluid. In addition, strain RO-07 exhibited strong resistance to aminoglycosides antibiotics. Furthermore, EPS extracted from RO-07 with a final concentration of 0.33 mg/mL showed antioxidant activities towards ·OH and O-2·, with scavenging capabilities of 75.2% and 95%, respectively. Therefore, EPS extracted from RO-07 were strongly anti-oxidative.
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