为了研发保鲜效果好的复合活性包装膜,将不同剂量的绿球藻多糖与壳聚糖复合制成可食活性膜,并测定了其物理性能(厚度、密度、溶解度、溶胀度、透明度、水蒸气透过率)和机械性能(抗拉强度、断裂伸长率),并对其结构进行了表征(红外光谱分析、X射线衍射分析、电子显微镜和原子力显微镜扫描),也考察了其1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)自由基的清除活性。结果表明,添加5~10 g/L的绿球藻多糖可增加壳聚糖复合膜厚度、密度、溶解度、溶胀度和DPPH自由基清除率,并减少水蒸气透过率,当绿球藻多糖为5 g/L时,透明度、抗拉强度和断裂伸长率均无显著差异。可见,绿球藻多糖作为天然活性物质能够很好地溶入壳聚糖膜中,形成紧密结构,制备成包装膜,提高复合膜的阻湿性能及抗氧化性,有利于其保鲜性能的提升。
In order to develop a composite packaging film with a good freshness-keeping effect, this study used different doses of Chlorococcum sp. GD polysaccharides composited with chitosan to make an edible active film. Its physical properties (thickness, density, solubility, swelling degree, opacity, water vapor permeability), and mechanical properties (tensile strength, elongation degree at break) were measured. Its structure was characterized by fourier transform infrared analysis, X-ray diffraction analysis, scanning electron microscope, and atomic force microscope scanning. Moreover, its ability to scavenge DPPH (1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl) was investigated. The results showed that adding 0.5%-1% of Chlorococcum sp. GD polysaccharides increased the thickness, density, solubility, swelling degree, and DPPH scavenging ability of the chitosan composite film. In addition, the water vapor permeability of the film was reduced. When 0.5% of Chlorococcum sp. GD polysaccharides was added, there were no significant differences in opacity, tensile strength, and elongation degree at break. The results indicated that as a natural active substance, Chlorococcum sp. GD polysaccharide could well dissolve into chitosan film to form a compact structure and prepare to be a packaging material to improve the moisture resistance and oxidative resistance of the composite film, which is beneficial to enhance its freshness-keeping effect.
[1] 周晓媛,蔡佑星,邓靖,等. 果蔬保鲜膜的保鲜机理与研究进展[J]. 食品研究与开发, 2008(11): 148-152.
[2] TAVASSOLI-KAFRANI E, SHEKARCHIZADEH H, MASOUDPOUR-BEHABADI M. Development of edible films and coatings from alginates and carrageenans [J]. Carbohydrate Polymers, 2016, 137(1): 360-374.
[3] DONHOWE I G, FENNEMA O. Edible films and coatings: Characteristics, formation, definitions, and testing methods [M]. Lancaster: Technomic Publishers, 1994.
[4] GENNADIOS A, WELLER C L. Edible films and coatings from wheat and corn proteins [J]. Food Technology, 1990, 44(10): 63-69.
[5] TOMIHATA K, IKADA Y. In vitro and in vivo degradation of films of chitin and its deacetylated derivatives[J]. Biomaterials, 1997, 18(7): 567.
[6] MOHAMED C, CLEMENTINE K A, DIDIER M, et al. Antimicrobial and physical properties of edible chitosan films enhanced by lactoperoxidase system [J]. Food Hydrocolloids, 2013, 30(2): 576-580.
[7] 李羚,高云涛,戴云,等. 螺旋藻及螺旋藻多糖体外清除活性氧及抗氧化作用研究[J]. 化学与生物工程, 2007, 24(3): 55-57.
[8] 周德庆,李振,柳淑芳,等. 藻类食品安全性评价与产品开发[J]. 中国食品学报, 2006, 6(1): 396-401.
[9] 夏冰,郭育涛,李晓晨. 螺旋藻多糖粗提的新方法工艺条件研究[J]. 应用化工, 2010, 39(6): 892-894.
[10] 张智宏. 壳聚糖/石榴皮提取物复合膜的制备及性能研究[D]. 昆明:昆明理工大学, 2013.
[11] ANDREUCCETTI C, CARVALHO R A, GALICIA-GARCíA T, et al. Effect of surfactants on the functional properties of gelatin-based edible films [J]. Journal of Food Engineering, 2011, 103(2): 129-136.
[12] SILVA M A D, BIERHALZ A C K, KIECKBUSCH T G. Alginate and pectin composite films crosslinked with Ca2+ ions: Effect of the plasticizer concentration [J]. Carbohydrate Polymers, 2009, 77(4): 736-742.
[13] PARK P J, JE J Y, KIM S K. Free radical scavenging activities of differently deacetylated chitosans using an ESR spectrometer [J]. Carbohydrate Polymers, 2004, 55(1): 17-22.
[14] MAYACHIEW P, DEVAHASTIN S. Effects of drying methods and conditions on release characteristics of edible chitosan films enriched with Indian gooseberry extract [J]. Food Chemistry, 2010, 118(3): 594-601.
[15] SRINIVASA P C, RAMESH M N, THARANATHAN R N. Effect of plasticizers and fatty acids on mechanical and permeability characteristics of chitosan films [J]. Food Hydrocolloids, 2007, 21(7): 1 113-1 122.
[16] 彭勇,李云飞,项凯翔. 绿茶多酚提高壳聚糖包装膜的抗氧化性能[J]. 农业工程学报, 2013, 29(14): 269-276.
[17] 全国塑料制品标准化技术委员会. GB 13022—91塑料薄膜拉伸性能试验方法[S]. 北京:中国标准化出版社, 1991.
[18] SIRIPATRAWAN U, HARTE B R. Physical properties and antioxidant activity of an active film from chitosan incorporated with green tea extract [J]. Food Hydrocolloids, 2010, 24(8): 770-775.
[19] BLOIS M S. Antioxidant determinations by the use of a stable free radical [J]. Nature, 1958, 181(4 617): 1 199-1 200.
[20] 中国国家标准化管理委员会. GB 10457—2009食品用塑料自粘保鲜膜[S]. 北京:中国标准化出版社, 2009.
[21] 方海峰. 壳聚糖/牛蒡/明胶/甘油复合膜制备及其在蓝莓保鲜中的应用[D]. 哈尔滨:东北林业大学, 2014.
[22] 刘晓菲,程春生,覃宇悦,等.增塑剂对壳聚糖/纳米蒙脱土复合膜物理性能的影响[J]. 食品与发酵工业, 2011, 37(2): 87-90.
[23] LECETA I, GUERRERO P, IBARBURU I, et al. Characterization and antimicrobial analysis of chitosan-based films [J]. Journal of Food Engineering, 2013, 116(4): 889-899.
[24] 王丽岩. 壳聚糖基活性包装膜的性能及其在食品贮藏中应用的研究[D]. 长春:吉林大学, 2013.
[25] 陈红,李彩云,李侠,等. 壳聚糖/微晶甾醇可食性复合膜的制备、性能及结构表征[J]. 食品科学, 2017, 38(9): 91-98.
[26] SOUZA A C D, DIAS A M A, SOUSA H C, et al. Impregnation of cinnamaldehyde into cassava starch biocomposite films using supercritical fluid technology for the development of food active packaging [J]. Carbohydrate Polymers, 2014, 102(4): 830-837.
[27] 刘伟. 姜酚抑菌作用及姜酚—壳聚糖复合膜性质与应用研究[D]. 北京:中国农业大学, 2015.
[28] XU Y X, KIM K M, HANNA M A, et al. Chitosan-starch composite film: preparation and characterization [J]. Industrial Crops & Products, 2005, 21(2): 185-192.
[29] 彭勇. 可食性壳聚糖活性包装膜成膜组分研究[D]. 上海:上海交通大学, 2014.
[30] CAVA D, CATALA R, GAVARA R, et al. Testing limonene diffusion through food contact polyethylene by FT-IR spectroscopy: Film thickness, permeant concentration and outer medium effects [J]. Polymer Testing, 2005, 24(4): 483-489.
[31] BANERJEE T, MITRA S, KUMAR S A, et al. Preparation, characterization and biodistribution of ultrafine chitosan nanoparticles [J]. International Journal of Pharmaceutics, 2002, 243(1-2): 93.
[32] 吴颖. 新型淀粉膜的制备及其结构和性能的研究[D]. 天津:天津大学, 2009.
[33] KANATT S R, CHANDER R, SHARMA A. Chitosan and mint mixture: A new preservative for meat and meat products [J]. Food Chemistry, 2008, 107(2): 845-852.
