食品与发酵工业 >

2019 , Vol. 45 >Issue 6: 231 - 235

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.018137

综述与专题评论

免疫分析技术在“新型瘦肉精”残留检测中的应用

  • 王耀 ,
  • 曹金博 ,
  • 陈曦 ,
  • 李燕虹 ,
  • 陈秀金 ,
  • 李兆周 ,
  • 邓瑞广 ,
  • 胡骁飞
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  • 1(河南科技大学 食品与生物工程学院,食品加工与安全国家级实验教学示范中心,河南 洛阳,471023)
    2(河南省农业科学院农业部动物免疫学重点实验室,河南省动物免疫学重点实验室,河南 郑州,450002)
博士(胡骁飞研究员为通讯作者,E-mail:huxf1972@126.com)。

收稿日期: 2018-06-27

  网络出版日期: 2019-04-18

基金资助

国家自然科学基金项目(31702218);国家科技支撑计划(20141BAD13B05);河南省科技攻关项目(182102110445);农业部动物免疫学重点实验室/河南省动物免疫学重点实验室开放课题(PKLAI20170603);河南科技大学博士科研启动基金项目(13480062)

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Application of immunoassay technique in detecting new type lean meat powder residues

  • WANG Yao ,
  • CAO Jinbo ,
  • CHEN Xi ,
  • LI Yanhong ,
  • CHEN Xiujin ,
  • LI Zhaozhou ,
  • DENG Ruiguang ,
  • HU Xiaofei
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  • 1(College of Food and Bioengineering, National Demonstration Center for Experimental Food Processing and Safety Education, Henan University of Science & Technology, Luoyang 471023, China)
    2 (Key Laboratory for Animal Immunology of the Ministry of Agriculture, Henan Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China)

Received date: 2018-06-27

  Online published: 2019-04-18

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摘要

近年来,一些“新型瘦肉精”药物被非法用于畜禽养殖,如苯乙醇胺A、赛庚啶、可乐定等,该类药物添加在饲料和动物饮水中能有效促进禽畜生长,提高瘦肉率,降低养殖成本,但其在肉制品中的残留会对人体产生蓄积毒性。免疫分析技术利用高亲和力抗体对抗原进行特异识别,灵敏度高,是目前快速检测方法研究的热点之一,已在“传统瘦肉精”的快速检测中成熟应用,但其在“新型瘦肉精”快速检测中的应用仍处于起步阶段。该文对免疫分析技术在“新型瘦肉精”残留检测中的应用进行综述,并对其未来发展方向进行展望。

关键词： 新型瘦肉精; 免疫分析; 应用

本文引用格式

王耀 , 曹金博 , 陈曦 , 李燕虹 , 陈秀金 , 李兆周 , 邓瑞广 , 胡骁飞 . 免疫分析技术在“新型瘦肉精”残留检测中的应用[J]. 食品与发酵工业, 2019 , 45(6) : 231 -235 . DOI: 10.13995/j.cnki.11-1802/ts.018137

Abstract

In recent years, some new types of lean meat powder are used illegally for livestock and poultry breeding, such as phenylethanolamine A, cyproheptadine, clonidine etc. These drugs added in animal feeds and drinking water can effectively promote the growth of livestock, improve the ratio of lean meat, and reduce the production cost. However, their residues in meat products could accumulate in human bodies and generate cumulative toxicity. Immunoassay technology uses high affinity antibodies to recognize antigen specifically and with high sensitivity. At present, it is one of the hot spots in fast detection methods. It has been used in rapid detection of traditional lean meat powder, but its application in detecting new lean meat powder is still in its infancy. In this paper, using immunoassay technique to detect new lean meat powder residues was reviewed, and its future development direction was prospected.

Key words： new type lean meat powder; immunoassay; application

参考文献

[1] 张改平,王选年,肖肖.瘦肉精的毒害作用及其试纸快速检测技术[J].中国动物检疫,2011,28(5):1-6.
[2] BI S,PANG B,WANG T,et al.Investigation on the interactions of clenbuterol to bovine serum albumin and lysozyme by molecular fluorescence technique[J].Spectrochimica Acta Part A Molecular & Biomolecular Spectroscopy,2014,120(24):456-461.
[3] BRAMBILLA G,CENCI T,FRANCONI F,et al.Clinical and pharmacological profile in a clenbuterol epidemic poisoning of contaminated beef meat in Italy[J].Toxicology Letters,2000,114(1-3):47-53.
[4] 王政,倪卫忠,严敏鸣.新型“瘦肉精”种类及其检测方法研究进展[J].上海畜牧兽医通讯,2013(2):30-31.
[5] KONISHI H,IGA I,NAGAI K.Underestimation of rat serum vancomycin concentrations measured by an enzyme-multiplied immunoassay technique and the strategy for its avoidance[J].Drug Testing & Analysis,2014,6(4):350.
[6] DAI M,GONG Y,LIU A,et al.Development of a colloidal gold-based lateral-flow immunoassay for the rapid detection of phenylethanolamine A in swine urine[J].Analytical Methods,2015,7(10):4 130-4 137.
[7] PAPICH M G.Cyproheptadine Hydrochloride[M].Fourth Edition. Saunders Handbook of Veterinary Drugs St. Louis: W.B. Saunders,2016:197-198.
[8] FIELDING S,LAL H.Clonidine: New research in psychotropic drug pharmacology[J].Medicinal Research Reviews,1981,1(1):97-123.
[9] HEETLA H W,STAAL M J,PROOST J H,et al.Clinical relevance of pharmacological and physiological data in intrathecal baclofen therapy[J].Archives of Physical Medicine & Rehabilitation,2014,95(11):2 199-2 206.
[10] MUHLENDAHL K E,KRIENKE E G.Toxicity of cyproheptadine. Side effects and accidental overdosage[J].Monatsschrift Fur Kinderheilkunde,1978,126(3):123-126.
[11] 王汉斌,熊锡山,孙成文,等.北京怀柔“4.23”急性可乐定中毒事件临床救治体会[J].中国科学:生命科学,2011,41(10):1 043-1 050.
[12] SEGER D L. Clonidine toxicity revisited[J].Journal of Toxicology Clinical Toxicology,2002,40(2):145-55.
[13] 王霞,康海燕.中枢性肌松剂巴氯芬临床应用及不良反应[J].临床医学,2011,31(2):114-115.
[14] YANG J,WANG Z,ZHOU T,et al.Determination of cyproheptadine in feeds using molecularly imprinted solid-phase extraction coupled with HPLC[J].Journal of Chromatography B,2015,990:39-44.
[15] YANG F,LIU Z,LIN Y,et al.Development an UHPLC-MS/MS method for detection of β-agonist residues in milk[J].Food Analytical Methods,2012,5(1):138-147.
[16] KARAMOLEGOU F,DASENAKI M,BELESSI V,et al.Multi-residue determination of 7 β-agonists in liver and meat using gas chromatography-mass spectrometry[J].Food Analytical Methods,2018,11(10):2 925-2 942.
[17] S′NIEGOCKI T,POSYNIAK A,Z·MUDZKI J.Improved gas chromatography-mass spectrometry method for the determination of clenbuterol and salbutamol in animal urine[J].Bulletin-Veterinary Institute in Pulawy,2005,49(4):443-447.
[18] OPLATOWSKA-STACHOWIAK M,REIRING C,SAJIC N,et al.Development and in-house validation of a rapid and simple to use ELISA for the detection and measurement of the mycotoxin sterigmatocystin[J].Analytical & Bioanalytical Chemistry,2018,410(12):3 017-3 023
[19] QU X,LIN H,DU S,et al.Development of a nano-gold capillary immunochromatographic assay for rapid and semi-quantitative detection of clenbuterol residues[J].Food Analytical Methods,2016,9(9):1-10.
[20] 刘硕,陶晓奇.β-兴奋剂在动物性食品中残留的免疫分析方法研究进展[J].食品与发酵工业,2017,43(8):273-279.
[21] LI Z,WANG Y,LI D,et al.Development of an indirect competitive enzyme-linked immunosorbent assay for screening ethopabate residue in chicken muscle and liver[J].RSC Advance,2017,7:36 072-36 080.
[22] LIU Z,ZHI A,ZHAO L,et al.Development of an ELISA for detection of Sudan I in food samples using monoclonal antibody[J].Food and Agricultural Immunology,2014,25(4):556-568.
[23] LI Y,LU S,LIU Z,et al.A monoclonal antibody based enzyme-linked immunosorbent assay for detection of phenylethanolamine A in tissue of swine[J].Food Chemistry,2015,167:40-44.
[24] WANG X,LIUFU T,BELOGLAZOVA N V,et al.Development of a competitive indirect enzyme-linked immunosorbent assay for screening phenylethanolamine A residues in pork samples[J].Food Analytical Methods,2016,9(11):3 099-3 106.
[25] 黄士新,李丹妮,顾欣,等.赛庚啶ELISA检测试剂盒的研制及其性能评价[J].中国兽药杂志,2016,52(11):44-48.
[26] 李丹妮,顾欣,黄华,等.可乐定ELISA检测试剂盒的研制及其性能评价[J].中国兽药杂志,2017,51(8):40-45.
[27] DZANTIEV B B,BYZOVA N A,URUSOV A E,et al.Immunochromatographic methods in food analysis[J].Trac Trends in Analytical Chemistry,2014,55(55):81-93.
[28] SUN Y,HU X,ZHANG Y,et al.Development of an immunochromatographic strip test for the rapid detection of zearalenone in corn[J].Journal of Agricultural and Food Chemistry,2014,62(46):11 116-11 121.
[29] ZHANG G P,WANG X N,YANG J F,et al.Development of an immunochromatographic lateral flow test strip for detection of β-adrenergic agonist Clenbuterol residues[J].Journal of Immunological Methods,2006,312(1-2):27-33.
[30] WANG Y,LI Z,PEI Y,et al.Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of soybean allergen β-conglycinin[J].Food Analytical Methods,2017,10(7):2 429-2 435.
[31] 聂雯莹,罗晓琴,李金超,等.动物尿液中苯乙醇胺A胶体金快速检测方法的建立[J].中国农业科学,2015,48(19):3 931-3 940.
[32] DAI M,GONG Y,LIU A,et al.Development of a colloidal gold-based lateral-flow immunoassay for the rapid detection of phenylethanolamine A in swine urine[J].Analytical Methods,2015,7(10):4 130-4 137.
[33] 付志锋,魏伟,李翠芳,等.电化学发光免疫传感技术在生物药物分析中的研究进展[J].中国科学:化学,2011, 41(5):20-31.
[34] WANG H,YUAN R,CHAI Y,et al.An ultrasensitive peroxydisulfate electrochemiluminescence immunosensor for Streptococcus suis serotype 2 based on l -cysteine combined with mimicking bi-enzyme synergetic catalysis to in situ generate coreactant[J].Biosensors & Bioelectronics,2013,43(15):63-68.
[35] LI J,GUO S,WANG E.Cheminform abstract: Recent advances in new luminescent nanomaterials for electrochemiluminescence sensors[J].Cheminform,2012,2(9):3 579-3 586.
[36] 济南大学.无标记电致化学发光瘦肉精免疫传感器的制备方法和应用:中国,201510034905.5[P].2015-10-21.
[37] 汤庆会.低毒性量子点和CdSe@SiO2的合成及用于电化学发光检测小分子物质[D].苏州:苏州大学,2015.
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