食品与发酵工业 >

2019 , Vol. 45 >Issue 7: 37 - 44

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.019164

研究报告

解脂亚罗酵母对棒曲霉素降解条件的筛选及部分机理研究

  • 胡慧敏 ,
  • 宗元元 ,
  • 王振宇 ,
  • 张国军 ,
  • 毕阳
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  • (甘肃农业大学 食品科学与工程学院,甘肃 兰州,730070)
硕士研究生(毕阳教授为通讯作者,E-mail:beyang62@163.com)。

收稿日期: 2018-10-26

  修回日期: 2018-12-27

  网络出版日期: 2019-05-14

基金资助

国家重点研发计划项目(2016YFD0400902);甘肃省高等学校科研项目(2016B-043);国家自然科学基金(地区科学基金项目)(31660476)

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Screening and partial mechanism analysis of patulin degradation conditions by Yarrowia lipolytica

  • HU Huimin ,
  • ZONG Yuanyuan ,
  • WANG Zhenyu ,
  • ZHANG Guojun ,
  • BI Yang
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  • (College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China)

Received date: 2018-10-26

  Revised date: 2018-12-27

  Online published: 2019-05-14

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摘要

通过解脂亚罗酵母(Yarrowia lipolytica)与棒曲霉素(patulin, pat)共培养,用高效液相色谱法(HPLC)测定棒曲霉素含量,研究该酵母对棒曲霉素的去除效果,用响应面法筛选最佳脱毒条件,并初步探讨脱毒机制。结果表明,Y. lipolytica能有效脱除棒曲霉素,脱毒效果受温度、pH值、棒曲霉素及酵母初始浓度和酵母活性的影响。在pH值4.6、温度27.5 ℃和酵母初始浓度1×1010 CFU/mL条件下,该酵母对棒曲霉素降解效果最佳,脱毒率可达100%。棒曲霉素对Y. lipolytica的生长具有一定的抑制,但整体抑制程度不大。棒曲霉素初始浓度越高,酵母的脱毒率就越低。酵母初始浓度越高,酵母的脱毒效果越好。活酵母对棒曲霉素的脱毒效果显著高于热致死酵母。此外,酵母胞内物质对棒曲霉素有显著的脱毒作用,而培养上清液对棒曲霉素没有作用。综上所述,Y. lipolytica可有效去除棒曲霉素,生物降解是其主要的作用机制。

关键词： 解脂亚罗酵母; 棒曲霉素; 脱毒; 影响因素

本文引用格式

胡慧敏 , 宗元元 , 王振宇 , 张国军 , 毕阳 . 解脂亚罗酵母对棒曲霉素降解条件的筛选及部分机理研究[J]. 食品与发酵工业, 2019 , 45(7) : 37 -44 . DOI: 10.13995/j.cnki.11-1802/ts.019164

Abstract

Yarrowia lipolytica was co-cultured with patulin. The detoxification effects of Y. lipolytica were investigated by measuring the concentrations of patulin by high performance liquid chromatography (HPLC). Response surface method was used to screen the optimal detoxification conditions. Besides, the mechanisms of detoxification were also examined. The results showed that Y. lipolytica effectively degraded patulin. Its detoxification effects were affected by temperature, pH, initial concentrations of patulin and yeast, as well as yeast activity. At pH=4.6 and 27.5 ℃ with the initial concentration of yeast being 1×1010 CFU/mL, the yeast exhibited the optimal degradation effect on patulin, as the degradation rate reached 100%. Patulin showed certain inhibitory effects on the growth of Y. lipolytica. The higher the initial concentration of patulin, the lower the detoxification rate of yeast. The higher the initial concentration of yeast, the better the detoxification effect. The detoxification effects of living yeast were significantly higher than that of heat-killed ones. Furthermore, its intracellular proteins showed significant detoxification effects than cell culture supernatant. Therefore, it is suggested that Y. lipolytica can effectively degrade patulin, and the major mechanism is by biodegradation.

Key words： Yarrowia lipolytica; patulin; detoxification; influence factors

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