通过研究在模拟胃肠消化体系中桑叶生物碱抗氧化活性的变化规律,为该物质在生物体内抗氧化作用研究提供参考。采用桑叶粉为原料,按料液比1∶30,60 ℃,功率800 W,超声处理60 min提取桑叶生物碱,测定其自由基清除能力、FRAP值、ORAC值、DNA和蛋白质氧化损伤保护潜力。结果表明,桑叶生物碱在模拟胃肠消化后,DPPH自由基清除能力、FRAP值极显著提高,是未消化时的3.88、2.44倍(P＜0.01);在油脂体系中具有较好的清除自由基作用,消化后其对β-胡萝卜素褪色的抑制率分别95.14%,是未消化时的6.12倍(P＜0.01);ORAC值显著提高到595.23 μmol Trolox/10 g md(P＜0.05),较未消化时增长了49.31%;桑叶生物碱经过胃肠消化后显著降低BSA羰基含量(P＜0.05),较未消化时减少了30.24%,且有效保护DNA不被损伤。基于研究结果,桑叶生物碱作为功能性食品(抗氧化剂)具有较大潜力。
The mulberry leaf alkaloids were ultrasonically extracted at 60 ℃ and 800 W for 60 min, and the ratio of solid to liquid was 1∶30. Its free radicals scavenging ability, values of FRAP and ORAC, and protective effects on protein and DNA oxidative damages were tested in simulated gastrointestinal digestion. After digestion, the DPPH free radical scavenging capacity and FRAP value of mulberry leaf alkaloids were significantly increased by 3.88 and 2.44 times of that before digestion (P<0.01). Besides, it had good free radical scavenging abilities in the oil system. After digestion, it inhibited the bleaching rate of β-carotene by 95.14%, which was 6.12 times higher than that before digestion (P<0.01). The ORAC value significantly increased to 595.23 μmol Trolox/10g md (P < 0.05), with an increase of 49.31% compared to undigested alkaloids. Moreover, the content of BSA carbonyl group significantly reduced after digestion (P < 0.05) by 30.24%. In addition, digested mulberry leaf alkaloids could effectively protect DNA against oxidative damage. The results indicated that mulberry leaf alkaloids have a great potential to be a functional healthy food as a natural antioxidant.
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