生产与科研经验

微波皂化法提取桔皮中类胡萝卜素及其组分分析

  • 方明 ,
  • 孙志高 ,
  • 于奉生 ,
  • 张群琳 ,
  • 李甜 ,
  • 盛冉 ,
  • 郝静梅
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  • 1(西南大学柑桔研究所,重庆,400712)
硕士研究生(孙志高副研究员为通讯作者,E-mail:cpro@cric.cn)。

收稿日期: 2018-10-10

  修回日期: 2018-10-19

  网络出版日期: 2019-05-14

基金资助

国家重点研发计划(2017YFD0400701-3)

Extraction of carotenoids from citrus peel by microwave-assisted saponification and its components

  • FANG Ming ,
  • SUN Zhigao ,
  • YU Fengsheng ,
  • ZHANG Qunlin ,
  • LI Tian ,
  • SHENG Ran ,
  • HAO Jingmei
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  • 1(Southwest University,Citrus Research Institute, Chongqing 400712,China)

Received date: 2018-10-10

  Revised date: 2018-10-19

  Online published: 2019-05-14

摘要

以南丰蜜桔皮为原料,采用微波皂化法提取桔皮类胡萝卜素,在单因素实验的基础上,通过Box-Behnken实验设计和响应面分析对微波皂化工艺进行了优化,利用C30柱及高效液相色谱-二极管阵列检测-大气压化学电离串联质谱方法对南丰蜜桔皮类胡萝卜素的组成进行分析,并对比考察了微波皂化与传统热皂化和冷皂化对桔皮主要类胡萝卜素组成及含量的影响。结果表明,微波皂化法提取类胡萝卜素的最佳工艺条件为:微波功率500 W、皂化时间10 min、KOH-乙醇溶液浓度1.0 mol/L,其类胡萝卜素得率为(0.709 6±0.11)‰;鉴定出南丰蜜桔皮中23种类胡萝卜素组分,其中β-隐黄素、黄体黄质、9-cis-紫黄质、di-cis-新黄质、花药黄质、叶黄素、玉米黄素为主要类胡萝卜素组分;微波皂化法所提取类胡萝卜素的含量显著高于传统热皂化和冷皂化,这3种皂化方式对含氧类胡萝卜素组成及含量影响较大。

本文引用格式

方明 , 孙志高 , 于奉生 , 张群琳 , 李甜 , 盛冉 , 郝静梅 . 微波皂化法提取桔皮中类胡萝卜素及其组分分析[J]. 食品与发酵工业, 2019 , 45(8) : 115 -122 . DOI: 10.13995/j.cnki.11-1802/ts.019018

Abstract

Carotenoids in Nanfeng mandarin peel were extracted by microwave-assisted saponification. Based on single factor experiments, microwave-assisted saponification process was optimized by BoxBehnken experimental design and response surface analysis. C30 column and high performance liquid chromatography (HPLC) coupled withdiode array detection (DAD) and atmospheric pressure chemical ioniation-tandem mass spectroscopy (APCI-MS) were applied to identify and quantify major carotenoids in Nanfengmandarin peel. Effects of microwave-assisted saponification and traditional thermal and cold saponification on the composition and contents of main carotenoids in citrus peel were compared.The results showed that the optimal conditions for extracting carotenoids by microwave-assisted saponification were as follows: microwaving at 500 W for 10 min, using 1.0 mol/L KOH-ethanol solution. The yields of carotenoids were (0.709 6±0.11)‰. Moreover, a total of 23 carotenoids were identified, with β-cryptoxanthin, luteoxanthin, 9-cis-violaxanthin, di-cis-neox-anthin, antherxanthin, lutein, zeaxanthin were the main components. The contents of carotenoids extracted by microwave-assisted saponification were significantly higher than that extracted by traditional thermal and cold saponification. These three saponification methods had great influences on the composition and contents of oxycarotenoids.

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