食品与发酵工业 >

2019 , Vol. 45 >Issue 8: 244 - 251

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.019609

综述与专题评论

益生菌喷雾干燥影响机制及优化策略分析

  • 肖怀秋 ,
  • 李玉珍 ,
  • 林亲录 ,
  • 赵谋明 ,
  • 刘军
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  • 1(湖南化工职业技术学院 制药与生物工程学院,湖南 株洲,412004)
    2(中南林业科技大学 食品科学与工程学院,湖南 长沙,410004)
    3(华南理工大学 食品科学与工程学院,广东 广州,510640)
副教授(李玉珍副教授为通讯作者,E-mail:yuzhenli@163.com)。

收稿日期: 2018-12-12

  修回日期: 2018-12-26

  网络出版日期: 2019-05-14

基金资助

湖南省自然科学基金科教联合基金项目(2017JJ5 020,2018JJ5022);湖南省教育厅科研项目(16C0550);湖南化工职业技术学院校级科研项目(HNHY2017013)

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Spray drying of probiotics, affecting factors, and optimization strategies

  • XIAO Huaiqiu ,
  • LI Yuzhen ,
  • LIN Qinlu ,
  • ZHAO Mouming ,
  • LIU Jun
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  • 1(School of Pharmeceutical and Bioegineering, Hunan Chemical Vocational Technology College, Zhuzhou 412004, China)
    2(College of Food Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China)
    3(School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China)

Received date: 2018-12-12

  Revised date: 2018-12-26

  Online published: 2019-05-14

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摘要

益生菌作为一种有益于肠道菌群的微生态制剂,在缓解急慢性胃肠炎、治疗腹泄、改善消化,缓解乳糖不耐症、减少病原菌于肠内的定植和生长、提升免疫力、缓解神经发育障碍等方面发挥重要作用。近些年,用喷雾干燥技术进行益生菌菌体干燥并对喷雾干燥工艺进行优化开展了大量的研究,取得了丰硕的成果。该文回顾了益生菌喷雾干燥的基本原理,并对影响益生菌喷雾干燥的因素进行了系统而全面分析。为提升益生菌喷雾干燥的活菌率,提出了益生菌喷雾干燥工艺优化策略,以期对益生菌的喷雾干燥研究起到推动作用。

关键词： 益生菌; 喷雾干燥技术; 影响机制; 优化策略

本文引用格式

肖怀秋 , 李玉珍 , 林亲录 , 赵谋明 , 刘军 . 益生菌喷雾干燥影响机制及优化策略分析[J]. 食品与发酵工业, 2019 , 45(8) : 244 -251 . DOI: 10.13995/j.cnki.11-1802/ts.019609

Abstract

Probiotics, a kind of microecological agents, are beneficial for intestinal flora and play important roles in alleviating acute and chronic gastroenteritis, treating abdominal diarrhea, improving digestion, alleviating lactose intolerance, reducing the colonization and growth of pathogenic bacteria in the intestine, enhancing immunity, and alleviating neurodevelopmental disorders. In recent years, many researches were conducted on parameters to optimize spray drying technology (SDT) to prepare dry probiotics, and significant progresses have been achieved. In this review, the basic principle of SDT to spray dry probiotics was summarized. The factors that affect SDT were systematically and comprehensively analyzed. The strategies for further optimization of SDT were prospected in order to improve the survival rate of probiotic during drying process, which aimed to promote the development of SDT.

Key words： probiotics; spray drying technology; affecting mechanisms; optimization strategies

参考文献

[1] FLOCH M H. The role of prebiotics and probiotics in gastrointestinal disease[J]. Gastroenterol Clin North Am, 2018, 47(1): 179-191.
[2] PAMER E G. Resurrecting the intestinal microbiota to combat antibiotic-resistant pathogens[J]. Science, 2016, 352(6 285): 535-538.
[3] GILBERT J A, KRAJMALNIKBROWN R, PORAZINSKA D L,et al. Toward effective probiotics for autism and other neuro- developmental disorders[J]. Cell, 2013, 155(7): 1 446-1 448.
[4] 傅楠, 陈晓东. 益生菌在喷雾干燥过程中的活性变化与保护策略[J].化工进展, 2018, 37(5): 1 633-1 645.
[5] ANANTA E,VOLKERT M,KNORR D.Cellular injuries and storage stability of spray-dried Lactobacillus rhamnosus GG[J]. International Dairy Journal, 2005, 15(4): 399-409.
[6] DESMOND C,ROSS R P,O′CALLAGHAN E O,et al. Improved survival of Lactobacillus paracasei NFBC 338 in spray-dried powders containing gum acacia[J]. Journal of applied microbiology, 2010, 93(6): 1 003-1 011.
[7] 陆英,陈卫,田丰伟,等. 利用热激处理提高益生菌Lactobacillus casei BD-Ⅱ抗热性的研究[J]. 中国乳品工业, 2006, 34(7): 17-19.
[8] TEIXEIRA P, CASTRO H, KIRBY R. Inducible thermotolerance in Lactobacillus bulgaricus[J]. Letters in applied microbiology, 2010, 18(4): 218-221.
[9] TRIPATHI M K, GIRI S K. Probiotic functional foods: Survival of probiotics during processing and storage[J]. Journal of Functional Foods, 2014, 9(9): 225-241.
[10] GOLOWCZYC M A, SILVA J, ABRAHAM A G,et al. Preservation of probiotic strains isolated from kefir by spray drying[J]. Letters in applied microbiology, 2010, 50(1): 7-12.
[11] CORCORAN B M, ROSS R P, FITZGERALD G F,et al. Comparative survival of probiotic lactobacilli spray-dried in the presence of prebiotic substances[J]. Journal of applied microbiology, 2010, 96(5): 1024-1 039.
[12] YANG YI, HUANG SONG, WANG JUAN,et al. Mg2+ improves the thermotolerance of probiotic Lactobacillus rhamnosus GG, Lactobacillus casei Zhang and Lactobacillus plantarum P-8[J]. Letters in applied microbiology, 2017, 64(4): 283-288.
[13] CARVALHO A S, SILVA J, HO P, et al. Effect of various growth media upon survival during storage of freeze-dried Enterococcus faecalis and Enterococcus durans[J]. Journal of Applied Microbiology, 2010, 94(6): 947-952.
[14] SILVA J, CARVALHO A S, PEREIRA H, et al. Induction of stress tolerance in Lactobacillus delbrueckii ssp. bulgaricus by the addition of sucrose to the growth medium[J]. Journal of Dairy Research, 2004, 71(1): 121-125.
[15] MOZZI F, ROLL N G,DE GIORI G S,et al. Effect of galactose and glucose on the exopolysaccharide production and the activities of biosynthetic enzymes in Lactobacillus casei CRL 87[J]. Journal of applied microbiology, 2010, 91(1): 160-167.
[16] KETS E, TEUNISSEN P, DE BONT J. Effect of compatible solutes on survival of lactic acid bacteria subjected to drying[J]. Appl Environ Microbiol, 1996, 62(1): 259-261.
[17] CORREA DEZA M A,GRILLO-PUERTAS M,SALVA S,et al. Inorganic salts and intracellular polyphosphate inclusions play a role in the thermotolerance of the immunobiotic Lactobacillus rhamnosus CRL 1505[J]. Plos One, 2017, 12(6): e0179242.
[18] AGUDELO J, CANO A,GONZÁLEZ-MARTÍNEZ C,et al. Disaccharide incorporation to improve survival during storage of spray dried Lactobacillus rhamnosus in whey protein-maltodextrin carriers[J]. Journal of Functional Foods, 2017(37): 416-423.
[19] LESLIE S B, ISRAELI E, LIGHTHART B,et al. Trehalose and sucrose protect both membranes and proteins in intact bacteria during drying[J]. Applied and environmental microbiology, 1995, 61(10): 3 592-3 597.
[20] BROECKX G,VANDENHEUVEL D, CLAES I J J,et al. Drying techniques of probiotic bacteria as an important step towards the development of novel pharmabiotics[J]. International journal of pharmaceutics, 2016, 505(1-2): 303-318.
[21] SALAR-BEHZADI S, WU SHENGQIAN, TOEGEL S,et al. Impact of heat treatment and spray drying on cellular properties and culturability of Bifidobacterium bifidum BB-12[J]. Food Research International,2013, 54(1): 93-101.
[22] SANTIVARANGKNA C, KULOZIK U, FOERST P. Alternative drying processes for the industrial preservation of lactic acid starter cultures[J]. Biotechnology Progress, 2010, 23(2): 302-315.
[23] 张建峰,耿宏伟,王丕武.酿酒活性干酵母生产工艺优化及干燥剂的选择[J]. 食品科学,2011,32(9): 213-216.
[24] KAILASAPATHY K. Protecting probiotics by microencapsulation[J]. Materials Transactions, 2018, 37(4): 738-742.
[25] SˇIPAILIENÈ A, PETRAITYTÈ S. Encapsulation of probiotics: Proper selection of the probiotic strain and the influence of encapsulation technology and materials on the viability of encapsulated micro- organisms[J]. Probiotics Antimicrob Proteins, 2018, 10(1): 1-10.
[26] SHORI A B. Microencapsulation improved probiotics survival during gastric transit[J]. Hayati Journal of Biosciences, 2017,24(1):1-5.
[27] SURONO I,VERHOEVEN J, VERBRUGGEN S, et al.Microencapsulation increases survival of the probiotic Lactobacillus plantarum IS-10506, but not Enterococcus faecium IS-27526 in a dynamic, computer controll- ed in vitro model of the upper gastrointestinal tract[J]. Journal of applied microbiology, 2018, 124(6): 1 604-1 609.
[28] 黄乐天,曹丁,黄魁英,等.微囊化蜡样芽孢杆菌的喷雾干燥工艺优化[J].安徽农业科学,2014(20):6 546- 6 548.
[29] 李宁,田洪涛,吴蕊,等. 双歧杆菌微胶囊喷雾干燥工艺的影响因素研究[J].食品与发酵工业,2007,33(8): 89-91.
[30] 谭文乐,肖更生,吴继军,等. 醋酸菌喷雾干燥工艺的研究[J].现代食品科技, 2010, 26(3): 272-276.
[31] 罗佳琦, 于晓晨, 于才渊. 嗜酸乳杆菌喷雾干燥技术的研究[J]. 干燥技术与设备, 2008(6): 273-278.
[32] BUSTAMANTE M, OOMAH B D, RUBILAR, MóNICA,et al. Effective Lactobacillus plantarum and Bifidobacterium infantis encapsulation with chia seed (Salvia hispanica L.) and flaxseed (Linum usitatissimum L.) mucilage and soluble protein by spray drying[J]. Food Chemistry, 2017, 216:97-105.
[33] EMILIE L, STÉPHANE G, CAROLINE P,et al. Cellular injuries in Cronobacter sakazakii CIP 103183T and Salmonella enterica exposed to drying and subsequent heat treatment in milk powder[J]. Frontiers in Microbiology, 2018, 9:475
[34] BOZA Y, BARBIN D,SCAMPARINI A R P. Effect of spray-drying on the quality of encapsulated cells of Beijerinckia sp[J]. Process Biochemistry, 2004, 39(10): 1 275-1 284.
[35] 郭云,孟祥晨.喷雾干燥对发酵乳杆菌KLDS1.0709存活影响的研究[J].食品工业科技,2011,32(2):109- 111.
[36] PARASTIWI ANDRIANI,EKOJONO.Design of spray dryer process control by maintaining outlet air temperature of spray dryer chamber[C]∥2016 International Seminar on Intelligent Technology and Its Applications [ISITIA]. IEEE, 2016: 619- 622
[37] RIVEROS B, FERRER J, B RQUEZ R. Spray drying of a vaginal probiotic strain of Lactobacillus acidophilus[J]. Drying Technology, 2009, 27(1): 123-132.
[38] KIM D H, LEE S B, PARK H D. Effect of air-blast drying and the presence of protectants on the viability of yeast entrapped in calcium alginate beads with an aim to improve the survival rate[J]. Applied Microbiology & Biotechnology, 2017, 101(1): 93-102.
[39] ZHOU X, DONG J, GAO J,et al. Activity-loss characteristics of spores of Bacillus thuringiensis during spray drying[J]. Food & Bioproducts Processing, 2008, 86(1): 37-42.
[40] DONZ E,BOIRON P,COURTHAUDON J L. Characterization of industrial dried whey emulsions at different stages of spray-drying[J]. Journal of Food Engineering, 2014, 126(1): 190-197.
[41] MENG X C, STANTON C,FITZGERALD G F,et al. Anhydrobiotics: The challenges of drying probiotic cultures[J]. Food Chemistry, 2008, 106(4): 1 406-1 416.
[42] TEIXEIRA P, CASTRO H, KIRBY R. Spray drying as a method for preparing concentrated cultures of Lactobacillus bulgaricus[J]. Journal of applied microbiology, 2010, 78(4): 456-462.
[43] ABE F, MIYAUCHI H, UCHIJIMA A,et al. Effects of storage temperature and water activity on the survival of bifidobacteria in powder form[J]. International Journal of Dairy Technology, 2010, 62(2): 234-239.
[44] MORGAN C A, HERMAN N, WHITE P A, et al. Preservation of micro-organisms by drying: A review[J]. J Microbiol Methods, 2006, 66(2): 183-193.
[45] SUNNY-ROBERTS E O, KNORR D. Protective effect of monosodium glutamate on survival of Lactobacillus rhamnosus GG and Lactobacillus rhamnosus E-97800 (E800) strains during spray-drying and storage in trehalose-containing powders[J]. International Dairy Journal, 2009, 19(4): 209-214.
[46] LIAN W C, HSIAO H C, CHOU C C. Survival of bifidobacteria after spray-drying[J]. International journal of food microbiology, 2002, 74(1-2): 79-86.
[47] HUANG SONG,CAUTY C, DOLIVET A,et al. Double use of highly concentrated sweet whey to improve the biomass production and viability of spray-dried probiotic bacteria[J]. Journal of Functional Foods, 2016, 23: 453-463.
[48] DIJKSTRA A R, ALKEMA W, STARRENBURG M J,et al. Fermentation-induced variation in heat and oxidative stress phenotypes of Lactococcus lactis MG1363 reveals transcriptome signatures for robustness[J]. Microbial Cell Factories, 2014, 13(1): 1-11.
[49] DESMOND C, STANTON C, FITZGERALD G F, et al. Environmental adaptation of probiotic lactobacilli towards improvement of performance during spray drying[J]. International Dairy Journal, 2002, 12(2-3): 183-190.
[50] GARDINER G E,O'SULLIVAN E, KELLY J,et al.Comparative survival rates of human-derived probiotic Lactobacillus paracasei and L. salivarius strains during heat treatment and spray drying[J]. Applied and environmental microbiology, 2000, 66(6): 2 605-2 612.
[51] BUCIO A, HARTEMINK R, SCHRAMA J W,et al. Survival of Lactobacillus plantarum 44a after spraying and drying in feed and during exposure to gastrointestinal tract fluids in vitro[J]. Journal of General and Applied Microbiology, 2005, 51(4): 221-227.
[52] REYES V, CHOTIKO A, CHOULJENKO A,etal. Influence of wall material on production of spray dried Lactobacillus plantarum NRRL B-4496 and its viability at different storage conditions[J]. Drying Technology, 2018,36 (14): 1 738-1 748.
[53] HERDEIRO R S, PEREIRA M D, PANEK A D,et al. Trehalose protects Saccharomyces cerevisiae from lipid peroxidation during oxidative stress[J]. BBA-General Subjects, 2006, 1 760(3): 340-346.
[54] ZHENG Xufeng, FU Nan, DUAN Manlei,et al. The mechanisms of the protective effects of reconstituted skim milk during convective droplet drying of lactic acid bacteria[J]. Food Research International, 2015,76(Pt 3): 478-488.
[55] SONG Huang, YI Yang, NAN Fu,et al. Calcium-aggregated milk: A potential new option for improving the viability of lactic acid bacteria under heat stress[J]. Food & Bioprocess Technology, 2014, 7(11): 3 147-3 155.
[56] WANG J, HUANG S, FU N,et al. Thermal aggregation of calcium-fortified skim milk enhances probiotic protection during convective droplet drying[J]. Journal of Agricultural & Food Chemistry,2016,64(30): 6 003.
[57] KHEM S,SMALL D M,MAY B K.The behaviour of whey protein isolate in protecting Lactobacillus plantarum [J]. Food chemistry, 2016(190): 717-723.
[58] 范娜, 陈雪峰. 益生菌抗热保护剂的研究[J]. 食品研究与开发, 2011, 32(4): 94-98.
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