研究了副溶血性弧菌在不同温度、pH值及NaCl浓度下的生物膜形成规律,采用微孔板法通过结晶紫染色测定生物膜形成量,苯酚硫酸法测定生物膜的胞外多糖,分析了不同的表面活性剂十二烷基硫酸钠(sodium dodecyl sulfate, SDS)、聚乙烯吡咯烷酮(polyvinyl pyrrolidone, PVP)、柠檬酸二铵对其生物膜形成的影响。结果表明,副溶血性弧菌在1%NaCl-pH 6、1% NaCl -pH 7、3% NaCl -pH 9、5% NaCl -pH 8条件下都能够形成生物膜,且3%NaCl和pH 9为强粘附,成膜最佳。37 ℃条件下,菌株成膜性最好,低温则有助于抑制生物膜的形成。SDS和PVP对生物膜的影响呈先促进后抑制的趋势,而柠檬酸二铵可促进生物膜的形成。同时,还分析了SDS促进菌膜形成的作用方式,明确600 mg/L的SDS对菌株胞外多糖分泌起促进作用,为病原微生物的防控提供了一定的理论基础。
The effects of temperature, pH, NaCl concentrations, and different surfactants (sodium dodecyl benzene sulfate (SDS), polyvinylpyrrolidone (PVP), and diammonium citrate) on the formation of Vibrio parahaemolyticus biofilms were studied. The amounts of biofilms formed were measured using microporous plates with crystal violet staining, and extracellular polysaccharides of biofilms were determined by phenol-sulfuric acid. The results showed that V. parahaemolyticus could form biofilms under the conditions of 1% NaCl-pH 6, 1% NaCl-pH 7, 3% NaCl-pH 9, and 5% NaCl-pH 8. Among which, biofilms formed under the condition of 3% NaCl-pH 9 was the best and had strong adhesiveness. The strain also formed good biofilms at 37 ℃ while low temperatures inhibited the formation of biofilms. Moreover, SDS and PVP firstly promoted then suppressed biofilm formation, while diammonium citrate showed promotive effect. Besides, 600 mg/L SDS promoted biofilm formation as it could promote the secretion of extracellular polysaccharides. In conclusion, this study provides a theoretical basis for preventing and controlling pathogens.
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