从皱纹盘鲍肠道中筛选出能够高效降解海带的菌株,并进行菌种鉴定及粗酶学性质研究。首先以褐藻酸钠为唯一碳源,筛选出褐藻胶裂解酶酶活较高的YY7菌株,通过16S rRNA初步鉴定YY7为弧菌属菌株;通过各种选择性培养基鉴定,YY7菌株不具有溶血性,产蛋白酶和淀粉酶,菌株生长速度快,其褐藻胶裂解酶活力在20 h达到最高54.31 U/mL,最适温度为35 ℃,最适pH 7.5,Na+、Mg2+、K+等金属离子对褐藻胶裂解酶活力具有促进作用,Fe2+和Cu2+起抑制作用。利用YY7菌株发酵液降解海带粉,降解得率可达65%以上,降解效果佳。
The strain that can efficiently degrade kelp was screened from the intestine of abalone. Identification and its enzymatic properties were investigated. Sodium alginate was used as the sole carbon source, and the strain YY7 with high alginate lyase activity was screened, which was further identified as Vibrio sp. by 16s rRNA gene sequence analysis. Additionally, various selective media showed that the strain YY7 was not hemolytic, and it also produced proteases and amylases. The growth rate of strain YY7 was fast, and its alginate lyase activity reached the highest of 54.31 U/mL in 20 h. The optimal reaction temperature of the alginate lyase was 35 ℃, and the optimum pH was 7.5. Metal ions such as Na+, Mg2+, and K+ etc. promoted the activity of the alginate lyase, while Fe2+ and Cu2+ inhibited its activity. The kelp powder was degraded by the fermentation broth of YY7 strain, and over 65% powder was degraded, which indicated that the degradation effect of strain YY7 was good. This study provides a basis for highly efficient degradation of kelp as well as for kelp deep processing.
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