研究报告

解淀粉芽孢杆菌胞外多糖对乳酸菌生长及代谢的调控作用

  • 蔡国林 ,
  • 刘逸凡 ,
  • 李晓敏 ,
  • 陆健
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  • 1(工业生物技术教育部重点实验室(江南大学),江苏 无锡,214122)
    2(粮食发酵工艺与技术国家工程实验室(江南大学),江苏 无锡,214122)
    3(江南大学 生物工程学院,江苏 无锡,214122)
博士研究生,副研究员(陆健教授为通讯作者,E-mail:jlu@jiangnan. edu.cn)。

收稿日期: 2018-12-29

  网络出版日期: 2019-06-17

基金资助

国家重点基础研究发展计划(973计划,2013CB7336 02);高等学校学科创新引智计划(111计划)资助项目(111-2-06);江苏高校优势学科建设工程资助项目

Effects of Bacillus amyloliquefaciens exopolysaccharides on proliferation and metabolism of lactic acid bacteria

  • CAI Guolin ,
  • LIU Yifan ,
  • LI Xiaomin ,
  • LU Jian
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  • 1(Key Laboratory of Industrial Biotechnology, Ministry of Education (Jiangnan University), Wuxi 214122, China)
    2(National Engineering Laboratory for Cereal Fermentation Technology (Jiangnan University), Wuxi 214122, China)
    3(School of Biotechnology,Jiangnan University, Wuxi 214122, China)

Received date: 2018-12-29

  Online published: 2019-06-17

摘要

研究解淀粉芽孢杆菌JN4的胞外多糖(exopolysaccharide, EPS-JN4)对乳酸菌生长及代谢的调控作用,有利于阐明EPS-JN4定向增殖乳酸菌的作用机制。采用PCR-变性梯度凝胶电泳技术分析EPS-JN4的定向增殖作用,并考察其对主要乳酸菌生长、主要代谢产物、肠道耐受性和表面疏水性的影响。EPS-JN4可以定向增殖罗伊氏乳杆菌,其对罗伊氏乳杆菌JN125的前期增殖速度较葡萄糖慢,但最高菌浓为葡萄糖的5.25倍,代谢产物中乳酸和醋酸含量则较葡萄糖分别降低了12.2%和32.4%。EPS-JN4增殖的罗伊氏乳杆菌JN125的肠道耐受性和表面疏水性显著提高,对其原因初步分析表明细胞膜蛋白和脂类物质含量提高了40.8%和105.7%,细胞膜组成中的十八碳脂肪酸和不饱和脂肪酸显著提高。EPS-JN4可以作为潜在的益生元,用于调节动物肠道健康。

本文引用格式

蔡国林 , 刘逸凡 , 李晓敏 , 陆健 . 解淀粉芽孢杆菌胞外多糖对乳酸菌生长及代谢的调控作用[J]. 食品与发酵工业, 2019 , 45(10) : 16 -21 . DOI: 10.13995/j.cnki.11-1802/ts.019814

Abstract

This study aimed to investigate the effects of exopolysaccharides from Bacillus amyloliquefaciens JN4 (EPS-JN4) on the propagation and metabolism of probiotics. The specific proliferative effects of EPS-JN4 were analyzed by PCR-denaturing gradient gel electrophoresis, and their effects on the growth, metabolites, intestinal tolerance, and surface hydrophobicity of lactic acid bacteria were also determined. It was found that EPS-JN4 had a specific proliferative effect on Lactobacillus reuteri. Compared to glucose, the early proliferation rate of L. reuteri JN125 treated with EPS-JN4 was slower, but its highest biomass was 4.25 times higher. In addition, the contents of lactic acid and acetic acid reduced by 12.2% and 32.4%, respectively. The intestinal tolerance and surface hydrophobicity of EPS-JN4-treated L. reuteri JN125 significantly improved, which could be due to the protein and lipid contents in cell membranes increased by 40.8% and 105.7%, respectively. Besides, the proportions of 18 C fatty acids and unsaturated fatty acids in cell membranes significantly increased. In conclusion, EPS-JN4 can be used as a potential prebiotic to regulate the intestinal health of animals.

参考文献

[1] SUTHERLAND I W. Novel and established applications of microbial polysaccharides[J]. Trends in Biotechnology, 1998, 16(1):41-46.
[2] ONER E T, HEMANDEZ L, COMBIE J. Review of Levan polysaccharide: From a century of past experiences to future prospects[J]. Biotechnology Advances, 2016, 34(5): 827-844.
[3] SCHMID J. Recent insights in microbial exopolysaccharide biosynthesis and engineering strategies[J]. Current Opinion in Biotechnology, 2018, 53:130-136.
[4] LIANG T W, WANG S L. Recent advances in exopolysaccharides from Paenibacillus spp.: Production, isolation, structure, and bioactivities[J]. Marine Drugs, 2015, 13(4): 1 847-1 863.
[5] BADEL S, BERNARDI T, MICHAUD P. New perspectives for Lactobacilli exopolysaccharides[J]. Biotechnology Advance, 2011, 29(1):54-66.
[6] FELS L, JAKOB F, VOGEL R F, et al. Structural characterization of the exopolysaccharides from water kefir[J]. Carbohydrate Polymers, 2018, 189: 296-303.
[7] ZHANG H, REN W, GUO Q B, et al. Characterization of a yogurt-quality improving exopolysaccharide from Streptococcus thermophilus AR333[J]. Food Hydrocolloids, 2018, 81, 220-228.
[8] YU X, AVALL-JAASKELAINEN S, KOOT J, et al. A comparative characterization of different host-sourced Lactobacillus ruminis strains and their adhesive, inhibitory, and immunomodulation functions[J]. Frontiers in Microbiology, 2017, 8:657.
[9] FUCHS V I, SCHMIDT J, SLATER M J, et al. Influence of immunostimulant polysaccharides, nucleic acids, and Bacillus strains on the innate immune and acute stress response in turbots (Scophthalmus maximus) fed soy bean- and wheat-based diets[J]. Fish Physiology and Biochemistry, 2017, 43(6): 1 501-1 515.
[10] POURABEDIN M, ZHAO X. Prebiotics and gut microbiota in chickens[J]. FEMS Microbiology Letters, 2015, 362(15): fnv122.
[11] GOULET O. Potential role of the intestinal microbiota in programming health and disease[J]. Nutrition Reviews, 2015, 73(Supp 1):32-40.
[12] SALAZAR N, GUEIMONDE M, DW LOS REYES-GAVILÁN C G, et al. Exopolysaccharides produced by Lactic acid bacteria and Bifidobacteria as fermentable substrates by the intestinal microbiota[J]. Critical Reviews in Food Science and Nutrition, 2016, 56(9):1 440-1 453.
[13] GOMAA E Z. Effect of prebiotic substances on growth, fatty acid profile and probiotic characteristics of Lactobacillus brevis NM101-1[J]. Microbiology, 2017, 86(5): 618-628.
[14] MOZZI F, GERBINO E, DE VADEZ G F, et al. Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system[J]. Journal of Applied Microbiology, 2009, 107(1): 56-64.
[15] TOLSTOGUZOV V. Why were polysaccharides necessary? [J]. Origins of Life and Evolution of Biospheres, 2004, 34(6): 571-597.
[16] 蔡国林, 冯文旭,刘逸凡,等. 高产抑制大肠杆菌血凝性的胞外多糖的解淀粉芽孢杆菌[J]. 食品与发酵工业,2019,45(5):14-18.
[17] JACOBS C M, UTTERBACK P L, PARSONS C M. Cecal microbial populations of young chicks fed several prebiotic-type compounds as determined by DGGE and quantitative PCR[J]. Journal of Dairy Science, 2010, 93:283-283.
[18] GOMAA E Z. Effect of prebiotic substances on growth, fatty acid profile and probiotic characteristics of Lactobacillus brevis NM101-1[J]. Microbiology, 2017, 86(5):618-628.
[19] DAS D, BARUAH R, GOYAL A. A food additive with prebiotic properties of an α-d-glucan from Lactobacillus plantarum DM5[J]. International Journal of Biological Macromolecules,2014, 69:20-26.
[20] 杨亚威, 赵爱梅,王辑,等. 传统奶豆腐中产胞外多糖乳酸菌的分离筛选及其潜在益生菌特性[J]. 中国乳品工业, 2015, 43(12):8-13.
[21] 邱然, 陆健. 啤酒污染菌的鉴定及其细胞膜脂肪酸的组成分析[J].食品与发酵工业, 2017, 43(7):55-61.
[22] DE PRETER V, HAMER H M, WINDEY K, et al. The impact of pre- and/or probiotics on human colonic metabolism: Does it affect human health?[J]. Molecular Nutrition and Food Research, 2011, 55(1):46-57.
[23] ZHANG B, WANG Y P, TAN Z F, et al. Screening of probiotic activities of Lactobacilli strains isolated from traditional Tibetan Qula, a raw yak milk cheese[J]. Asian-Australasian Journal of Animal Sciences, 2016, 29(10):1 490-1 499.
[24] SUZUKI K,IIJIMA K,SAKAMOTO K,et al. A review of hop resistance in beer spoilage lactic acid bacteria[J]. Journal of the Institute of Brewing, 2006, 112(2):173-191.
[25] MYKYTCZUK N C S, TREYORS J T, LEDUC L G, et al. Fluorescence polarization in studies of bacterial cytoplasmic membrane fluidity under environmental stress [J]. Progress in Biophysics and Molecular Biology, 2007, 95(3):60-82.
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