为筛选出可高效利用棉籽糖的菌种并对其酶学性质进行研究,采用高效液相色谱法测定了棉籽糖含量,平板计数法测定菌种生长量,pNPG法测定α-半乳糖苷酶酶活。结果显示,该实验筛选出屎肠球菌、粪肠球菌和凝结芽孢杆菌3株可以高效利用棉籽糖的菌种,发酵后培养基中棉籽糖剩余含量分别为0、0.36%和1.05%(质量分数)。屎肠球菌α-半乳糖苷酶最适pH值为5,最适温度为45 ℃。粪肠球菌α-半乳糖苷酶最适pH为5,最适温度为50 ℃。凝结芽孢杆菌α-半乳糖苷酶最适pH为8,最适温度为45 ℃。所产α-半乳糖苷酶具有较为稳定的酶学性质,可为今后饲料和食品中棉籽糖的去除提供研究方向。
This study aimed to screen strains that could efficiently utilize raffinose and to illustrate their enzymatic characteristics. The raffinose content, strain growth, and α-galactosidase activity were measured. The isolates that could efficiently utilize raffinose belonged to Enterococcus faecium, Enterococcus faecalis, and Bacillus coagulans. The residual contents of raffinose in the medium after fermenting with E. faecium, E. faecalis, and B. coagulans were 0, 0.36%, and 1.05%, respectively. The optimum pH of the α-galactosidase from E. faecium, E. faecalis and B. coagulans were 5, 5, and 8, respectively, and the optimum temperatures were 45 ℃, 50 ℃ and 45 ℃, respectively. This study provides research directions for removing raffinose from feeds and foods in the future.
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