食品与发酵工业 >

2019 , Vol. 45 >Issue 12: 1 - 6

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.019910

研究报告

两阶段溶氧控制及FeSO4添加对谷氨酸棒杆菌合成4-羟基异亮氨酸的影响

  • 孟静 ,
  • 芦楠 ,
  • 朱福周 ,
  • 董解荣 ,
  • 王子申 ,
  • 陈宁 ,
  • 张成林
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  • 1.天津科技大学 生物工程学院,天津,300457；
    2.菱花集团有限公司,山东 济宁,272073
硕士研究生(张成林副教授为通讯作者,E-mail:zcl@tust.edu.cn)。

收稿日期: 2019-01-10

  修回日期: 2019-03-27

  网络出版日期: 2019-07-16

基金资助

国家自然科学基金项目(31300069, 31770053);中国博士后科学基金项目(2017M611170, 2018T110662);天津市科技计划项目(17YFZCSY01050);天津科技大学青年教师创新基金项目(2016LG07)

收起

Effects of two-stage DO control and FeSO4 addition on 4-hydroxyisoleucine production by Corynebacterium glutamicum

  • MENG Jing ,
  • LU Nan ,
  • ZHU Fuzhou ,
  • DONG Jierong ,
  • WANG Zishen ,
  • CHEN Ning ,
  • ZHANG Chenglin
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  • 1. College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;
    2. Linghua Group Ltd, Jining 272073, China

Received date: 2019-01-10

  Revised date: 2019-03-27

  Online published: 2019-07-16

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摘要

优化4-羟基异亮氨酸发酵过程中溶氧水平及FeSO4添加量,以提高其发酵水平。结合菌株Corynebacterium glutamicum HIL18及4-羟基异亮氨酸合成特点,首先考察不同溶氧水平及两阶段溶氧控制对4-羟基异亮氨酸发酵的影响。然后考察FeSO4添加对其发酵的作用。20%溶氧有利于4-羟基异亮氨酸的合成。利用两阶段溶氧控制工艺(0～20 h、20%溶氧;20～64 h、30%溶氧)经64 h发酵,4-羟基异亮氨酸产量达到38.7 g/L,较未使用该工艺提高11.2%。采用两阶段FeSO4添加策略(初始浓度为65 μmol/L、20 h添加30 μmol/L),4-羟基异亮氨酸的产量达到43.4 g/L。采用优化后的工艺使得4-羟基异亮氨酸产量较优化前提高27.3%。获得了4-羟基异亮氨酸发酵过程中两阶段溶氧控制及FeSO4添加工艺。该结果为4-羟基异亮氨酸的高效发酵合成提供参考。

关键词： 4-羟基异亮氨酸; 谷氨酸棒杆菌; 溶氧; 异亮氨酸羟化酶

本文引用格式

孟静 , 芦楠 , 朱福周 , 董解荣 , 王子申 , 陈宁 , 张成林 . 两阶段溶氧控制及FeSO4添加对谷氨酸棒杆菌合成4-羟基异亮氨酸的影响[J]. 食品与发酵工业, 2019 , 45(12) : 1 -6 . DOI: 10.13995/j.cnki.11-1802/ts.019910

Abstract

Effects of DO levels and two-stage DO control strategy on 4-hydroxyisoleucine production were investigated, followed by analyzing the effects of two-stage FeSO4 addition. The results indicated that 20% DO was suitable for 4-hydroxyisoleucine synthesis during one-stage DO control process. During two-stage DO control process (20% DO for 0-20 h and 30% for 20-64 h), 38.7 g/L 4-hydroxyisoleucine was obtained, which was 11.2% higher than that at 20% DO. By using two-stage FeSO4 addition strategy (65 μmol/L for primary concentration and 30 μmol/L FeSO4 added at 20 h), the production of 4-hydroxyisoleucine increased to 43.4 g/L, which was 27.3% higher than the control. In conclusion, the strategy of two-stage DO control and FeSO4 addition provides a reference for highly efficient production of 4-hydroxyisoleucine.

Key words： 4-hydroxyisoleucine; Corynebacterium glutamicum; dissolved oxygen; isoleucine dioxygenase

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