通过建立洛哌丁胺诱导的小鼠便秘模型,评价凝结芽孢杆菌B.C-39芽孢与低聚果糖、低聚半乳糖、菊粉的复合微生态制剂对便秘的缓解效果。结果表明,与模型组相比,复合微生态制剂显著增加了便秘小鼠的排便颗粒数、粪便质量和水分含量,小肠推进率提高了43.9%,排首粒黑便的时间缩短了125 min,同时显著恢复了小鼠血清中胃动素(motilin,MTL)、胃泌素(gastrin,Gas)、内皮素(endothelin-1,ET-1)、生长抑素(somatostatin,SS)、P 物质(substance P,SP)和血管活性肠肽(vasoactive intestinal peptide,VIP)等胃肠调节肽的分泌水平,提高了小鼠粪便中短链脂肪酸的含量。凝结芽孢杆菌B.C-39复合微生态制剂具有缓解便秘的作用,为开发、应用具有缓解便秘功能的凝结芽孢杆菌微生态制剂提供了理论依据
A mouse model of loperamide-induced constipation was established to evaluate the effects of Bacillus coagulans B.C-39 spores containing oligofructose, galactooligosaccharides and inulin against constipation. A series of constipation related indicators were detected . The results showed that compared with the model group, the compound microecological preparation could significantly increase the fecal pellet numbers, fecal weight and water content of defecation. Meanwhile, the intestinal transit rate increased by 43.9%, and the first black stool defecation time was shortened by 125 minutes.The compound microecological preparation could also restore the levels of gastrointestinal regulatory peptides such as motilin (MTL), gastrin (Gas), endothelin (ET-1), somatostatin (SS), substance P (SP), and vasoactive intestinal peptide (VIP). Furthermore, feeding with compound microecological preparation increased the content of short chain fatty acids in mouse feces. These results showed that Bacillus coagulans B.C-39 compound microecological preparation could relieve constipation effectively.The results provide a theoretical basis for the development and application of Bacillus coagulans microecological preparations for relieving constipation.
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