食品与发酵工业 >

2019 , Vol. 45 >Issue 13: 257 - 261

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.020370

综述与专题评论

杀灭芽孢杆菌的方法及机理的研究综述

  • 刘仁杰 ,
  • 梁珊 ,
  • 李哲 ,
  • 朴春红
展开
  • 吉林农业大学 食品科学与工程学院,吉林 长春,130118
博士研究生,副教授(朴春红教授为通讯作者,E-mail:piaochunhong9111@163.com)

收稿日期: 2019-02-27

  网络出版日期: 2019-07-28

基金资助

吉林省科技厅项目(20160519013JH);吉林省科技厅项目(20170312022ZX)

收起

Methods and mechanisms for killing Bacillus: a review

  • LIU Renjie ,
  • LIANG Shan ,
  • LI Zhe ,
  • PIAO Chunhong
Expand
  • College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118,China

Received date: 2019-02-27

  Online published: 2019-07-28

Fold

摘要

芽孢杆菌在不利条件下产生内生芽孢,芽孢壁厚且对外界环境有较强抵抗力,常规杀菌方法不易将其杀灭。芽孢在食品中萌发形成营养细胞,严重降低食品安全性,威胁消费者健康。文章从芽孢杆菌在食品中产生的危害出发,对热杀菌、高压杀菌、气体杀菌、化学杀菌以及促萌发杀菌等方法进行分析,比较其杀灭芽孢杆菌的效果以及优缺点。并对其杀菌机理进行探讨,指出膜受体、离子通道、核蛋白以及酶类对芽孢杆菌的影响。以期为芽孢杆菌的研究和控制提供理论参考和技术支撑。

关键词： 芽孢杆菌; 杀菌方法; 杀菌机理

本文引用格式

刘仁杰 , 梁珊 , 李哲 , 朴春红 . 杀灭芽孢杆菌的方法及机理的研究综述[J]. 食品与发酵工业, 2019 , 45(13) : 257 -261 . DOI: 10.13995/j.cnki.11-1802/ts.020370

Abstract

Bacillus can produce endogenous spores under adverse conditions. As the spore wall is thick and has strong resistance to external environment, therefore, Bacillus is difficult to be killed by conventional sterilization methods. The nutrient cells produced in foods when spores germinating seriously affect food safety and threaten consumers' health. Based on the damages caused by Bacillus, this paper analyzed various sterilization methods, such as heating, high pressure, gas sterilization, chemical sterilization, and germination sterilization etc. The effects of these methods were compared and their advantages and disadvantages to kill Bacillus were analyzed. Besides, the effects of membrane receptors, ion channels, nuclear proteins and enzymes on Bacillus were pointed out, in order to provide theoretical reference and technical support for researching and controlling Bacillus.

Key words： Bacillus; sterilization method; bactericidal mechanism

参考文献

[1] 王春,王芊,曹旭.大豆内生菌的分离及菌核病拮抗菌的筛选[J].黑龙江农业科学,2015(4):27-30.
[2] 安红梅.三峡库区耐淹植物内生真菌重金属抗性研究[D].重庆:西南大学, 2015.
[3] 陈丽华, 袁德超,吴毅歆,等. 棉花黄萎病生防内生芽孢杆菌LH-L3的分离鉴定[J]. 棉花学报, 2017, 29(6):550-559.
[4] 陈艳光.枯草芽孢杆菌BSD-2诱导黄瓜抗灰霉病作用机理研究[D]. 石家庄:河北师范大学, 2015.
[5] 李继兵.五株芽孢杆菌新种的分离纯化及其鉴定研究[D].上海:上海海洋大学, 2015.
[6] VENKATESWARAN K, CHECINSKA S A, RATNAYAKE S, et al. Draft genome sequences from a novel clade of Bacillus cereus Sensu Lato strains, isolated from the international space station [J]. Genome Announcements, 2017, 5(32):e00 680-17.
[7] NAGLER K, SETLOW P, LI Y Q, et al. High salinity alters the germination behavior of Bacillus subtilis spores with nutrient and nonnutrient germinants [J]. Applied & Environmental Microbiology, 2014, 80(4):1 314-1 321.
[8] BUDKA H, BUNCIC S, COLION P, et al. Opinion of the scientific panel on biological hazards (BIOHAZ) on Bacillus cereus and other Bacillus spp. in foodstuffs[J]. The EFSA Journal, 2005, 3(4):1-48.
[9] DZIECIOL M, FRICKER M, WANGNER M, et al. A novel diagnostic real-time PCR assay for quantification and;differentiation of emetic and non-emetic Bacillus cereus[J]. Food Control, 2013, 32(1):176-185.
[10] BWATTIE S H, WILLIAMS A G. Detection of toxigenic strains of Bacillus cereus and other Bacillus spp. with an improved cytotoxicity assay[J]. Letters in Applied Microbiology, 2010, 28(3):221-225.
[11] REDONDO-SOLANO M, VALENZUELA-MARTINEZ C, CASSADA D A, et al. Effect of meat ingredients (sodium nitrite and erythorbate) and processing (vacuum storage and packaging atmosphere) on germination and outgrowth of Clostridium perfringens spores in ham during abusive cooling.[J]. Food Microbiology, 2013, 35(2):108-115.
[12] 罗立荣,徐倩.新生儿急性坏死性小肠结肠炎的防治效果观察[J].中国城乡企业卫生,2018,33(5):51-52.
[13] DZIECIOL M, FRICKER M, WAGNER M, et al. A novel diagnostic real-time PCR assay for quantification and differentiation of emetic and non-emetic Bacillus cereus[J]. Food Control, 2013, 32(1):176-185.
[14] BAG A, CHATTOPADHYAY R R. Synergistic antibacterial and antibiofilm efficacy of nisin in combination with p-Coumaric acid against foodborne bacteria Bacillus cereus and Salmonella typhimurium[J]. Letters in Applied Microbiology, 2017, 65(5):366-372.
[15] AOUADHI C, MEJRI S, MAAROUFI A. Inhibitory effects of nisin and potassium sorbate alone or in combination on vegetative cells growth and spore germination of Bacillus sporothermodurans in milk[J]. Food Microbiology, 2015, 46:40-45.
[16] MCKILLIP J L. Prevalence and expression of enterotoxins in Bacillus cereus and other Bacillus spp., a literature review [J]. Antonie van Leeuwenhoek, 2000, 77(4): 393-399.
[17] 郭丹丹.脉冲强磁场对牛奶中微生物和酶的作用研究[D].广州:华南理工大学,2010.
[18] 秦雯娜, 徐新磊,郭立忠,等. 地衣芽孢杆菌XJ-2菌株产胞外多糖发酵条件的优化及其抗氧化活性研究[J]. 四川农业大学学报, 2014, 32(2):165-171.
[19] 赵宏强, 蓝蔚青,张皖君,等. 超高压技术在水产品杀菌保鲜中的研究进展[J]. 食品工业科技, 2016, 37(22):369-373.
[20] 黄训端,潘见,谢慧明,等.高静压对蜡样芽孢杆菌的致死与损伤效应[J].核农学报,2006,20(6):511-515.
[21] 高鑫, 梅俊,李博. 臭氧在食品工业中的应用研究进展[J]. 粮食与饲料工业, 2017, 12(2):35-39.
[22] 耿淑洁,胡学香,胡春,等.臭氧-氯联合灭活饮用水中枯草芽孢杆菌芽孢的研究[J].环境工程学报,2011, 5(3):489-493.
[23] 吴丽樱.豆腐主要腐败菌的研究及其来源分析[D]. 无锡:江南大学, 2017.
[24] 吕世明,陈杖榴,陈建新,等.丁香酚体外抑菌作用研究[J].食品科学,2008,29(9):122-124.
[25] 韩乾杰, 张玲玲,陈敏,等. 植物精油与丁酸钠的体外协同抑菌效果研究[J]. 动物营养学报, 2017, 29(2):712-718.
[26] 倪萍,刘素辉,牛婷婷,等.紫皮蒜和白皮蒜抑菌活性的比较研究[J].医学信息,2013(16):126-126.
[27] 李静,马媛.鱼腥草乙醇浸提液对体外细菌抑制的对比研究[J].江苏农业科学,2011(1):373-374.
[28] TEHRI N, KUMAR N, RAGHU H V, et al. Role of stereospecific nature of germinants in Bacillus megaterium spores germination[J]. Biotech, 2017, 7(4):259.
[29] AOUADHI C, SIMONIN H, MAAROUFI A, et al. Optimization of nutrient-induced germination of Bacillus sporothermodurans spores using response surface methodology[J]. Food Microbiology, 2013, 36(2):320-326.
[30] HO S Y, MITTAL G S, CROSS J D. Effects of high field electric pulses on the activity of selected enzymes [J]. Journal of Food Engineering, 1997, 31(1):69-84.
[31] 上海康久消毒技术中心. 臭氧杀菌消毒机在特医食品安全质量改善中的应用[J]. 食品工业科技, 2016, 37(22):42-43.
[32] 李凤娟.高压CO2对枯草芽孢杆菌芽孢的灭活效能及机理研究[D].合肥:合肥工业大学,2013.
[33] 霍健聪,邓尚贵,励建荣.鱼腥草黄酮的制备及其对枯草芽孢杆菌的抑制机理[J].中国食品学报,2017,17(9):82-89.
[34] 王晓英.蒲公英总黄酮对假单胞菌抑菌机理的探讨[J].食品研究与开发,2012,33(11):18-21.
[35] 王涛.食品组分与抑菌剂对嗜热脂肪芽孢杆菌芽孢耐热性的影响[D].无锡:江南大学, 2011.
[36] SETLOW P. Summer meeting 2013-when the sleepers wake: the germination of spores of Bacillus species[J]. Journal of Applied Microbiology, 2013, 115(6):1 251-1 268.
[37] MOIR A. How do spores germinate?[J]. Journal of Applied Microbiology, 2010, 101(3):526-530.
[38] IGARASHI T, SETLOW P. Interaction between individual protein components of the GerA and GerB nutrient receptors that trigger germination of Bacillus subtilis spores[J]. Journal of Bacteriology, 2005, 187(7):2 513-2 518.
[39] LOVDAL I S, FROM C, MADSLIENad E H, et al. Role of the gerA operon in L-alanine germination of Bacillus licheniformis spores[J]. BMC Microbiology, 2012, 12(1):34.
[40] RAMIREZPERALTA A, STEWART K A V, THOMAS S K, et al. Effects of the SpoVT regulatory protein on the germination and germination protein levels of spores of Bacillus subtilis[J]. Journal of Bacteriology, 2012, 194(13):3 417-3 425.
[41] BEHRAVAN J, CHIRAKKAL H, MASSON A, et al. Mutations in the gerP Locus of Bacillus subtilis and Bacillus cereus affect access of germinants to their targets in spores[J]. Journal of Bacteriology, 2000, 182(7):1 987-1 994.
[42] CORTEZZO D E, SETLOW B, SETLOW P. Analysis of the action of compounds that inhibit the germination of spores of Bacillus species [J]. Journal of Applied Microbiology, 2004, 96(4):725-741.
[43] JOHNSON C L, MOIR A. Proteins YlaJ and YhcN contribute to the efficiency of spore germination in Bacillus subtilis [J]. FEMS Microbiology Letters, 2017, 364(7).
[44] SOUTHWORTH T W, GUFFANTI A A, MOIR A, et al. GerN, an endospore germination protein of Bacillus cereus, is an Na+/H+-K+ antiporter[J]. Journal of Bacteriology, 2001, 183(20):5 896-5 903
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司