生产与科研应用

阿魏酸酯酶对晒青毛茶发酵过程的影响

  • 雷林超 ,
  • 赵明双 ,
  • 龙宣蓉 ,
  • 陈培钦 ,
  • 李夏兰
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  • 华侨大学 化工学院,福建 厦门,361000
硕士研究生(李夏兰教授为通讯作者,E-mail:xialan@hqu.edu.cn)

收稿日期: 2018-12-06

  网络出版日期: 2019-08-20

基金资助

福建省自然科学基金项目(No.2017J01065);福建省大学生创新创业训练计划项目(No.201810385090);华侨大学研究生科研创新基金资助项目(No.17013087013)

Effects of ferulic acid esterase on fermentation process of sun-dried tea

  • LEI Linchao ,
  • ZHAO Mingshuang ,
  • LONG Xuanrong ,
  • CHEN Peiqin ,
  • LI Xialan
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  • College of Chemical Engineering, Huaqiao University,Xiamen 361000, China

Received date: 2018-12-06

  Online published: 2019-08-20

摘要

以晒青毛茶为原料,研究阿魏酸酯酶对晒青毛茶发酵过程中活性物质的影响。利用高效液相色谱法以及分光光度计法测定茶叶中各种活性物质含量的变化。实验结果表明,发酵8 d后茶叶的水浸出物、还原糖、阿魏酸及低聚木糖均比晒青毛茶高,茶汤的pH值及游离氨基酸含量下降。在晒青毛茶中同时添加3 mL(75 U/mL)阿魏酸酯酶和3 mL(75 U/mL)木聚糖酶,在发酵第8天阿魏酸及低聚木糖分别可达 838.9 μg/g及3.78 μg/g,比不添加任何酶的茶样分别高20.9%及38.6%。在晒青毛茶发酵过程中,同时添加阿魏酸酯酶与木聚糖酶有利于提高茶叶的品质,从而提升茶叶的附加值。该研究结果对发酵型茶的保健功效提供一定的理论依据。

本文引用格式

雷林超 , 赵明双 , 龙宣蓉 , 陈培钦 , 李夏兰 . 阿魏酸酯酶对晒青毛茶发酵过程的影响[J]. 食品与发酵工业, 2019 , 45(14) : 128 -132 . DOI: 10.13995/j.cnki.11-1802/ts.019553

Abstract

The effects of ferulic acid esterase on active components of sun-dried tea during fermentation were studied. After 8 days enzymatic fermentation, the levels of water extract, reducing sugars, ferulic acid and xylooligosaccharides increased, while pH and free amino acid decreased in comparison to sun-dried tea. Ferulic acid esterase (75 U/mL) and xylanase (75 U/mL) resulted the contents of ferulic acid and xylooligosaccharides raised up to 838.9 and 3.78 μg/g, respectively, which were 20.9% and 38.6% higher than the control, respectively. Therefore, ferulic acid esterase and xylanase are beneficial to improve the quality of the tea and increase the added value during sun-dried tea fermentation. This study provides a theoretical basis for revealing health benefits of fermented tea.

参考文献

[1] ZHENG W J, WAN X C, BAO G H. Brick dark tea: A review of the manufacture,chemical constituents and bioconversion of the major chemical components during fermentation[J].Phytochemistry Reviews,2015, 14(3):499-523.
[2] ZHU Y F, CHEN J J, JI X M, et al.Changes of major tea polyphenols and production of four new B-ring fission metabolites of catechins from post-fermented Jing-Wei Fu brick tea[J]. Food Chemistry, 2015, 170: 110-117.
[3] ABE M,TAKAOKA N,IDEMOTO Y, et al.Characteristic fungi observed in the fermentation process for Puer tea[J]. International Journal of Food Microbiology, 2008,124(2):199-203.
[4] LI Q, CAI S, LI Y, et al. Biochemical components associated with microbial community shift during the pile-fermentation of primary dark tea [J].Frontiers in Microbiology,2018, 9(1 509):1-13.
[5] ZHANG W,YANG R,FANG W,et al.Characterization of thermophilic fungal community associated with pile fermentation of Pu-erh tea[J]. International journal of food microbiology, 2016, 227:29-33.
[6] DAMÁSIO A R L,BRAGA C M P. Biomass-to-bio-products application of feruloyl esterase from Aspergillus clavatus[J].Appl Microbiol Biotechnol,2013, 97(15):6 759-6 767.
[7] BRAGA C M P, DA-SILVA D P, DA-SILVA L D J, et al.Addition of feruloyl esterase and xylanase produced on-site improves sugarcane bagasse hydrolysis[J]. Bioresource Technology,2014,170(5):316-324.
[8] OLIVEIRA D M, MOTA T R, OLIVA B.et al. Feruloyl esterases:Biocatalysts to overcome biomass recalcitrance and for the production of bioactive compounds[J].Bioresource Technology,2019,278:408-423.
[9] 刘闫,胡茂丰,刘素纯.冠突散囊菌产阿魏酸酯酶及其发酵麸皮和黑毛茶形成阿魏酸的研究[J].农产品加工,2015,4(8):1-3;6.
[10] BEGOÑA B, GÓMEZ-CORDOVÉS C,SANCHO A I,et al.Growth and release of hydroxycinnamic acids from Brewer’s spent grain by Streptomyces avermitilis CECT 3339[J].Enzyme and Microbial Technology, 2003, 32(1):140-144.
[11] DE-OLIVEIRA D M,SALVADOR V H, MOTA T R,et al.Feruloyl esterase from Aspergillus clavatus improves xylan hydrolysis of sugarcane bagasse[J].AIMS Bioengineering, 2017,4 (1): 1-11.
[12] YU P, MCKINNON J J, CHRISTENSEN D A. Improving the nutritional value of oat hulls for ruminant animals with pretreatment of a multienzyme cocktail: In vitro studies[J].Journal of Animal Science 2005, 5 (83):1 133-1 141.
[13] 李夏兰,程珊影,杨道秀,等.阿魏酸酯酶和木聚糖酶协同降解麦糟[J].化工进展,2012, 31(5):1 096-1 102;1 108.
[14] 罗云,王镇发,梅胜,等.阿魏酸酯酶酶化发酵饲料饲喂三元猪的影响[J].食品与生物技术学报, 2018,37(5):527-534.
[15] 郭桂义,葛国平.不同茶类茶汤pH值的研究[J].食品科技,2012,37(5):74-76.
[16] 中华人民共和国国家质量监督检验检疫总局. GB/T 8305—2013,茶水浸出物测定[S].北京:中国标准出版社,2013.
[17] 李环,陆佳平,王登进.DNS法测定山楂片中还原糖含量的研究[J].食品工业科技,2013,34(18):75-77.
[18] 中华人民共和国国家质量监督检验检疫总局.GB/T 8314—2013,茶水浸出物测定[S].北京:中国标准出版社,2013.
[19] 李夏兰,范韵敏,方柏山.来自桔青霉的阿魏酸酯酶的分离纯化、理化性质[J].微生物学报,2010,50(8):1 058-1 064.
[20] 中华人民共和国国家质量监督检验检疫总局.GB/T 23776—2018,茶水浸出物测定[S].北京:中国标准出版社,2018.
[21] 白玉艳,赵艳,王白娟.云南勐库大叶种普洱茶水浸出物含量的研究[J].食品工业,2013(6):14-16.
[22] 张秀秀.外源氨基酸和糖对普洱茶品质的影响[D]. 广州:华南理工大学,2012.
[23] 吕海鹏,谷记平,林智,等.普洱茶的化学成分及生物活性研究进展[J].茶叶科学,2007,27(1):8-18.
[24] 郭桂义,邵静娜,王在群,等.信阳毛尖茶汤pH值的初步研究[J].食品科技,2011,36(3):92-94.
[25] 刘盼盼,钟小玉,许勇泉,等.茶叶中有机酸及其浸出特性研究[J].茶叶科学,2013,33(5):405-410.
[26] ZHANG L,ZHANG Z Z,ZHOU Y B,et al.Chinese dark teas: Postfermentation, chemistry and biological activities[J]. Food Research International,2013, 53(2):600-607.
[27] 冯超浩,刘通讯.不同潮水量条件下普洱茶渥堆过程化学成分的变化[J].食品科学,2013,34(7):135-139.
[28] YU P, MAENZ D D, MCKINNON J J, et al. Release of ferulic acid from oat hulls by Aspergillus ferulic acid esterase and Trichoderma xylanase[J].Journal of Agricultural & Food Chemistry,2002, 50(6):1 625-1 630.
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