以曙红为探针,利用其与恩诺沙星形成的新缔合物的光学性质,实现对恩诺沙星的准确且灵敏检测。在酸性条件下,恩诺沙星与曙红相互反应而形成的新缔合物使曙红的最大吸收波长从514 nm红移至520 nm处,且520 nm波长处的吸光度随着恩诺沙星浓度的增加而减小。基于此而建立了一种曙红探针光度法检测恩诺沙星含量的新方法。在优化的实验条件下,恩诺沙星浓度在0.08~10.0 g/mL范围内与体系在520 nm处的吸光度呈良好的线性关系(R2=0.992 4),方法的检出限与定量限分别为0.05 g/mL、0.08 g/mL。对1.0 g/mL的恩诺沙星进行连续8次测定,其RSD为4.9%。利用本方法对鸡肉与鸡蛋中恩诺沙星含量进行测定并做回收率实验,平均回收率分别为93.61%与92.17%。该方法有望为食品监督部门提供一种测定食品中恩诺沙星含量的新途径。
关键词：
曙红; 恩诺沙星; 探针光度法; 鸡蛋; 鸡肉 ; ;
Eosin and enrofloxacin can form a complex that can shift the maximum absorption wavelength of eosin from 514 nm to 520 nm in acidic media, and the absorbance at 520 nm decreases with increasing amount of enrofloxacin. Based on this principle, current study established a new accurate and sensitive method to detect enrofloxacin by using eosin as a probe. Under optimal conditions, the absorbance at 520 nm had a good linear relationship with 0.08-10.0 μg/mL enrofloxacin (R2=0.992 4). Moreover, this method had 0.05 μg/mL detection limit, 0.08 μg/mL quantitation limit, and the relative standard deviation was 4.9%. Furthermore, the average recovery rates of the method for detecting enrofloxacin in chicken and eggs were 93.61% and 92.17%, respectively. Overall, this proposed method offers a new tool for food supervision authority to quantify enrofloxacin in foods.
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