研究报告

甘南传统牦牛酸奶来源抗氧化性乳酸发酵菌株的筛选

  • 张俊 ,
  • 王炳文 ,
  • 赵保堂 ,
  • 何兴芬 ,
  • 王娇 ,
  • 杨富民
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  • 1甘肃农业大学 食品科学与工程学院,甘肃 兰州,730070
    2甘肃农业职业技术学院,甘肃 兰州,730020
硕士研究生(杨富民教授为通讯作者,E-mail:yfumin@163.com)。

网络出版日期: 2019-09-03

基金资助

甘肃省科技计划重点研发计划(18YF1NA076);甘肃特色农畜产品加工技术研究(GSAU-ZL2015-048);甘肃省青年科技基金计划(17JR5RA152)

Antioxidative lactic acid fermenting strains screening from traditional yak yogurt in Gannan

  • ZHANG Jun ,
  • WANG Bingwen ,
  • ZHAO Baotang ,
  • HE Xingfen ,
  • WANG Jiao ,
  • YANG Fumin
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  • 1College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China
    2Gansu Agriculture Technology College,Lanzhou 730020, China

Online published: 2019-09-03

摘要

为寻找甘南传统牦牛酸奶中具有优良发酵与抗氧化性能的乳酸菌,对分离纯化的菌株以H2O2耐受力、耐酸、耐胆盐和凝乳发酵性进行初步筛选,再以1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)、羟基(·OH)自由基和超氧阴离子(O-2·)的清除率筛选抗氧化能力强的菌株。结果表明,所筛选出的6株乳酸菌中,菌株M5的发酵性能与抗氧化活性最强,生理生化及分子生物学特征鉴定结果为副干酪乳杆菌。该菌株凝乳发酵时间为6.2 h;上清液对DPPH和羟自由基清除率分别为47.8%和54.4%;无细胞提取物对超氧阴离子的清除率为49.9%。酵母存活细胞模型验证,其无细胞提取物、发酵上清液、完整细胞对酿酒酵母的存活率分别提高了95.3%、130%和28.5%。试验所筛选出的副干酪乳杆菌M5的发酵及抗氧化能力强,具有进一步开发利用价值。

本文引用格式

张俊 , 王炳文 , 赵保堂 , 何兴芬 , 王娇 , 杨富民 . 甘南传统牦牛酸奶来源抗氧化性乳酸发酵菌株的筛选[J]. 食品与发酵工业, 2019 , 45(15) : 74 -80 . DOI: 10.13995/j.cnki.11-1802/ts.020373

Abstract

This study aimed to screen lactic acid bacteria with excellent fermentation and antioxidant capacity from Gannan traditional yak's yoghurt. The isolated and purified strain was screened by H2O2-resistant capacity, acid resistance, bile salt resistance and curd fermentation, followed by evaluating their scavenging effects on DPPH·, ·OH and O-2·. The results showed that the strain M5 had the strongest fermentation performance and antioxidant activity. The strain M5 was identified as Lactobacillus paracasei by physiological, biochemical and molecular biological characterization. The curd fermentation time of this strain was 6.2 h, and the DPPH· and OH· scavenging rates of the fermentation supernatant were 47.8% and 54.4%, respectively. Moreover, the cell-free extract could scavenge O-2· by 49.9%. The yeast viable cell model verified that the cell-free extract, fermentation supernatant and intact cells increased the survival rate of Saccharomyces cerevisiae by 95.3%, 130%, and 28.5%, respectively. Therefore, L. paracasei M5 has strong fermentation and antioxidant capacity, which has further development and utilization value.

参考文献

[1] 武万强, 王琳琳,赵建新,等.植物乳杆菌生理特性及益生功能研究进展[J]. 食品与发酵工业, 2019,45(1):1-13.
[2] SWETWIWATHANA A, VISESSANGUAN W. Potential of bacteriocin-producing lactic acid bacteria for safety improvements of traditional Thai fermented meat and human health[J]. Meat Science, 2015, 109:101-105.
[3] LIU H, GONG J S, CHABOT D, et al. Improved survival of Lactobacillus zeae LB1 in a spray dried alginate-protein matrix[J]. Food Hydrocolloids, 2018, 78: 100-108.
[4] ISHII N. Oxidative stress and aging in Caenorhabditis elegans[J]. Free Radical Research, 2000, 33(6): 857-864.
[5] MULLER F L, LUSTGARTEN M S, JANG Y, et al. Trends in oxidative aging theories[J]. Free Radical Biology and Medicine, 2007, 43(4): 477-503.
[6] LUO D H, FANG B S. Structural identification of ginseng polysaccharides and testing of their antioxidant activities[J]. Carbohydrate Polymers, 2008, 72(3): 376-381
[7] DAS D, GOYAL A. Antioxidant activity and γ-aminobutyric acid (GABA) producing ability of probiotic Lactobacillus plantarum DM5 isolated from Marcha of Sikkim[J]. LWT-Food Science and Technology, 2015, 61(1): 263-268.
[8] DING W R, WANG L N, ZHANG J, et al. Characterization of antioxidant properties of lactic acid bacteria isolated from spontaneously fermented yak milk in the Tibetan Plateau[J]. Journal of Functional Foods, 2017, 35: 481-488.
[9] SHAKIBAIE M, MOHAMMADI-KHORSAND T, ADELI-SARDOU M, et al. Probiotic and antioxidant properties of selenium-enriched Lactobacillus brevis LSe isolated from an Iranian traditional dairy product[J]. Journal of Trace Elements in Medicine and Biology, 2017, 40: 1-9.
[10] LIU D M, WANG P, ZHANG X Y, et al. Characterization of nitrite degradation by Lactobacillus casei subsp. rhamnosus LCR 6013[J]. PLoS one, 2014, 9(4): e93 308.
[11] 杜瑞平,郭帅,潘娜,等. 乳酸菌胞外多糖益生功能研究进展[J].动物营养学报,2018,30(7):2 439-2 444.
[12] HUANG Y H, LAI Y J, CHOU C C. Fermentation temperature affects the antioxidant activity of the enzyme-ripened sufu, an oriental traditional fermented product of soybean[J]. Journal of bioscience and bioengineering, 2011, 112(1): 49-53.
[13] 刘慧.现代食品微生物学试验技术[M].北京:中国轻工出版社, 2006:143-163.
[14] 洪松虎. 抗氧化活性乳酸菌的筛选及其在水产品加工贮藏中的应用[D]. 宁波:宁波大学, 2009.
[15] 王桢, 李阳,车帅,等. 北极海洋沉积物高抗氧化活性菌株的筛选及其多样性分析[J]. 海洋科学进展, 2015, 33(1):63-70.
[16] 周晏阳, 孔雪英,吴梅,等.1株牦牛源产细菌素植物乳杆菌的益生特性分析[J]. 食品科学, 2018, 39(14):132-137.
[17] 罗璠,杨丽雪,魏婕,等. 不同原料乳对发酵酸奶品质的影响[J].西南民族大学学报(自然科学版),2018,44(1):14-18.
[18] ZHANG L, LIU C, LI D, et al. Antioxidant activity of an exopolysaccharide isolated from Lactobacillus plantarum C88[J]. International Journal of Biological Macromolecules, 2013, 54(1): 270-275.
[19] LI S, ZHAO Y, ZHANG L, et al. Antioxidant activity of Lactobacillus plantarum strains isolated from traditional Chinese fermented foods[J]. Food Chemistry, 2012, 135(3): 1 914-1 919.
[20] 陈明, 柯文灿,保安安,等. 青藏高原牦牛酸奶中具高抗氧化能力乳酸菌的筛选[J].食品工业科技,2016,37(8):201-205.
[21] 林祥娜,夏永军,王光强,等. 抗氧化活性乳酸菌的筛选[J]. 中国食品学报,2017,17(6):103-109.
[22] 凌代文,东秀珠. 乳酸细菌分类鉴定及试验方法[M]. 北京:中国轻工业出版社,1993: 6.
[23] FONTANA L, PARTRIDGE L, LONGO V D. Extending healthy life span—from yeast to humans[J]. Science, 2010, 328(5976): 321-326.
[24] BURTNER C R, MURAKAMI C J, KENNEDY B K, et al. A molecular mechanism of chronological aging in yeast[J]. Cell cycle, 2009, 8(8): 1 256-1 270.
[25] 巨晓英,寇晓虹.副干酪乳杆菌的功能及其在食品工业中的应用研究进展[J].中国乳品工业,2009,37(8):48-50.
[26] ANCOS B D, PILAR C M, G MEZ R. Characteristics of stirred low-fat yoghurt as affected by high pressure[J]. International Dairy Journal, 2000, 10(1):105-111.
[27] 华鹤良. 乳酸菌的分离鉴定及其抗菌肽与发酵性能研究[D].扬州:扬州大学,2014.
[28] REJINIEMON T S, HUSSAIN R R, RAJAMANI B. In-vitro functional properties of Lactobacillus plantarum isolated from fermented ragi malt[J]. South Indian Journal of Biological Sciences, 2015, 1(1): 15-23.
[29] 张凤敏,田丰伟,陈卫,等. 具抗氧化活性乳酸菌的筛选[J]. 中国乳品工业, 2007, 35(2):4-7.
[30] CHOORUK A, PIWAT S, TEANPAISAN R. Antioxidant activity of various oral Lactobacillus strains[J]. Journal of applied microbiology, 2017, 123(1): 271-279.
[31] SHI Y, CUI X, GU S, et al. Antioxidative and probiotic activities of lactic acid bacteria isolated from traditional artisanal milk cheese from northeast China[J]. Probiotics and antimicrobial proteins, 2018: 1-14.
[32] KUDA T, KANEKO N, YANO T, et al. Induction of superoxide anion radical scavenging capacity in Japanese white radish juice and milk by Lactobacillus plantarum isolated from aji-narezushi and kaburazushi[J]. Food Chemistry, 2010, 120(2): 517-522.
[33] SUO H, LIU S, LI J, et al. Lactobacillus paracasei ssp. paracasei YBJ01 reduced d-galactose–induced oxidation in male Kuming mice[J]. Journal of dairy science, 2018, 101(12): 10 664-10 674.
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