研究报告

日龄对雄性伊拉兔肌肉蛋白质组成的影响

  • 李少博 ,
  • 贺稚非 ,
  • 胡颖 ,
  • 王泽富 ,
  • 李洪军
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  • 1西南大学 食品科学学院,重庆,400716
    2重庆市特色食品工程技术研究中心,重庆,400716
博士研究生(李洪军教授为通讯作者,E-mail:983362225@qq.com)。

网络出版日期: 2019-09-03

基金资助

2017年国家自然科学基金项目(31671787);国家兔产业技术体系肉加工与综合利用(CARS-43-E-1);重庆市草食牲畜产业技术体系(Y201706);重庆市研究生科研创新项目(CYB18099)

Effects of days of age on meat protein profile in male Ira rabbits

  • LI Shaobo ,
  • HE Zhifei ,
  • HU Ying ,
  • WANG Zefu ,
  • LI Hongjun
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  • 1College of Food Science, Southwest University, Chongqing 400716, China
    2Chongqing Engineering Research Center of Regional Food, Chongqing 400716, China

Online published: 2019-09-03

摘要

为了解家兔在生长过程中蛋白质组成的变化情况,研究了35、70和105日龄雄性伊拉兔肌肉中肌浆蛋白、肌原纤维蛋白和肌基质蛋白的组成比例变化情况,并运用shotgun蛋白质组学技术深入探讨了兔肉的蛋白质组成。结果显示,兔肉中含有较高的蛋白质和必需氨基酸,日龄会显著影响部分氨基酸的含量,如亮氨酸和苏氨酸等;其次,兔肉中肌浆蛋白、肌原纤维蛋白和肌基质蛋白占总蛋白质的平均比例分别为32.32%、57.35%和10.33%,日龄会显著影响肌基质蛋白的含量;另外,在兔肉中共检测出790种蛋白质,这些蛋白质主要参与机体的能量代谢、物质运输和生化反应等,其中35日龄兔肉中蛋白质的数量显著高于其他2个日龄。以上结果表明,日龄会显著影响兔肉的蛋白质组成,这为伊拉兔在生长过程中兔肉品质变化的研究提供了方向。

本文引用格式

李少博 , 贺稚非 , 胡颖 , 王泽富 , 李洪军 . 日龄对雄性伊拉兔肌肉蛋白质组成的影响[J]. 食品与发酵工业, 2019 , 45(15) : 93 -99 . DOI: 10.13995/j.cnki.11-1802/ts.020327

Abstract

To determine changes in protein composition of rabbit meat during growth, male Ira rabbits aged 35 d, 70 d and 105 d were used to evaluate their protein quantity (sarcoplasmic, myofibrillar, and stromal protein) and protein profiles. The results indicated that Ira rabbit meat was rich in protein and essential amino acids, and age significantly affected the amino acid profile of rabbit meat, such as Leu and Thr. Moreover, the proportions of sarcoplasmic, myofibrillar, and stromal protein accounted for 32.32%, 57.35%, and 10.33%, respectively, of total protein, and days of age had a significant effect on the content of stromal protein. Furthermore, there were 790 proteins identified in samples using shotgun proteomics, which were mainly involved in energy metabolism, material transport and biochemical reactions etc., and the protein content in the meat of 35-day-old Ira rabbit was the highest among all groups. In conclusion, age has significant effects on protein composition of rabbit meat, which provides a direction for researching changes in Ira rabbit meat quality at growth stage.

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