为高效利用口虾蛄制备金属硫蛋白(metallothionein, MT),以口虾蛄MT提取量为指标,考察提取时间、提取温度、液浓度、液料比、提取液浓度和pH值5个因素对MT提取效果的影响,并用响应面法研究口虾蛄中MT的最佳提取工艺条件。结果表明,口虾蛄MT最佳提取工艺为:提取时间1 h、提取温度51 ℃、液料比4∶1、提取液浓度0.02 mol/L、提取液pH 7.8;在此条件下MT的提取量为0.232 mg/g,该结果与理论预测值基本相符。响应面法建立的优化模型能较好地预测口虾蛄MT的提取量,这为后续对口虾蛄MT进行分离、纯化研究提供基础,为海洋生物资源的开发和利用提供新的思路。
The purpose of this study was to use Oratosquilla oratoria as a raw material to prepare metallothionein. The extraction yield of metallothionein was used as an evaluation indicator, effects of extraction time, extraction temperature, liquid-to-solid ratio, buffer concentration, and pH of extract solution were investigated. It was found that extracted at 51 ℃ for 1 h, with 4∶1 liquid to solid ratio, 0.02 mol/L buffer and an extract solution with pH=7.8 was the optimum process to extract Oratosquilla oratoria metallothionein. Under this condition, the amount of Oratosquilla oratoria metallothionein extracted was 0.232 mg/g, which was consistent with the theoretical value. Therefore, the optimization model established by response surface method could be used to estimate the amount of metallothionein extracted from Oratosquilla oratoria, which provides a basis for separating and purifying Oratosquilla oratoria metallothionein in the following research, and also gives a new idea for developing and utilizing marine resources.
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