食品与发酵工业 >

2019 , Vol. 45 >Issue 15: 157 - 163

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.020591

生产与科研应用

酒酒球菌(Oenococcus oeni)产胞外聚合物培养基的优化及其抗冷冻干燥性能评价

  • 王继锋 ,
  • 石侃 ,
  • 安玮 ,
  • 余东亮 ,
  • 刘树文 ,
  • 何玲
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  • 1西北农林科技大学 葡萄酒学院,陕西 杨凌,712100
    2陕西省葡萄与葡萄酒工程技术研究中心,陕西 杨凌,712100
    3陕西省合阳葡萄试验示范站,陕西 渭南,715300
    4秦皇岛金士国际葡萄酒庄有限公司,河北 昌黎,066600
    5西北农林科技大学 园艺学院,陕西 杨凌,712100
硕士研究生(刘树文教授和何玲教授为共同通讯作者,E-mail:liushuwen@nwsuaf.edu.cn,heliurui@nwsuaf.edu.cn)。

网络出版日期: 2019-09-03

基金资助

贺兰山东麓特色葡萄酒生产工艺体系及葡萄酒标准体系建立研究(2016BZ0603);“十三五”国家科技支撑计划项目(2016YFD0400504-01);国家现代农业产业技术体系建设专项(nycytx-30-ch-03)

收起

Culture medium optimization for Oenococcus oeni producing extracellular polymeric substances and their cryoprotective ability

  • WANG Jifeng ,
  • SHI Kan ,
  • AN Wei ,
  • YU Dongliang ,
  • LIU Shuwen ,
  • HE Ling
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  • 1College of Enology, Northwest A&F University, Yangling 712100, China
    2Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, China
    3Heyang Experimental and Demonstrational Stations for Grape, Weinan 715300, China
    4Chateau Kings International Co., Ltd. Qinhuangdao, Changli 066600, China
    5College of Horticulture, Northwest A&F University, Yangling 712100, China

Online published: 2019-09-03

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摘要

为了研究酒酒球菌(Oenococcus oeni)产胞外聚合物(extracellular polymeric substances, EPS)在冷冻干燥过程中的保护性能,通过优化培养基提高EPS产量,并通过EPS对细菌冷冻干燥存活率的影响以及观察冷冻干燥过程中酒酒球菌内部及外表面的变化,评价其抗冷冻干燥的性能。正交试验优化可得,在初始pH值4.8、蛋白胨含量20 g/L、葡萄糖含量15 g/L的条件下,能产119.7 mg/100 mL酒酒球菌EPS。EPS作为冷冻干燥保护剂时细菌冷冻干燥后的存活率为70.97%,高于其他常规保护剂,扫描电子显微镜(scanning electron microscope,SEM)和透射电子显微镜(transmission electron microscope,TEM)的图像表明添加冷冻干燥保护剂的实验组细胞形态更加完整,说明EPS有明显的冷冻干燥保护作用。该研究初步探究了酒酒球菌EPS冷冻干燥保护的机制,并为EPS在冷冻干燥保护剂中的应用提供理论基础。

关键词： 酒酒球菌; 胞外聚合物; 冷冻干燥; 存活率

本文引用格式

王继锋 , 石侃 , 安玮 , 余东亮 , 刘树文 , 何玲 . 酒酒球菌(Oenococcus oeni)产胞外聚合物培养基的优化及其抗冷冻干燥性能评价[J]. 食品与发酵工业, 2019 , 45(15) : 157 -163 . DOI: 10.13995/j.cnki.11-1802/ts.020591

Abstract

This study aimed to optimize the culture medium for producing extracellular polymeric substances (EPS) by Oenococcus oeni and evaluate the cryoprotective ability of EPS by scanning electron microscope (SEM), transmission electron microscope (TEM) and the survival rate analysis of O. oeni after freeze-drying. It was found that 119.7 mg/100 mL EPS was produced under the optimized condition: an initial pH of 4.8 with 20 g/L peptone and 15 g/L glucose. The survival rate of bacteria after freeze-drying was 70.97% when EPS was used as a cryoprotectant, which was higher than those of other conventional cryoprotectants. Besides, EPS had obvious protective effects on freeze-dried bacteria, as the morphology of cells in experimental groups with cryoprotectants were more complete. This study prelimin.arily explored the mechanisms of EPS regarding protecting freeze-dried O. oeni, which provides a theoretical basis for applying EPS in developing freeze-drying protectants.

Key words： Oenococcus oeni; extracellular polymeric substances; freeze-drying; survival rate

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