为了研究桑葚提取物中花青素的组成、含量及其体外抗氧化活性,采用高效液相色谱与质谱联用法对桑葚提取物中的花青素组成进行鉴定。以矢车菊素-3-O-葡萄糖苷为标准品测定了花青素的含量,并测定桑葚提取物的体外抗氧化活性。结果表明,桑葚提取物的总花青素含量为314.30 μg/mg,其中共有3种花青素,分别为矢车菊素-3-O-葡萄糖苷、矢车菊素-3-O-芸香糖苷和矢车菊素;体外抗氧化试验表明,桑葚提取物的DPPH、ABTS+和羟基自由基清除能力的IC50值分别为22.33、12.90和1.74 mg/mL,在桑葚提取物质量浓度为250 μg/mL时,其还原力达到0.744。桑葚提取物中花青素含量高,且具有良好的抗氧化活性,该研究为桑葚提取物的开发利用提供了理论依据。
The composition and content of anthocyanins in mulberry extract and their in vitro antioxidant activities were determined. The composition of anthocyanins was identified by HPLC-MS, and the content of anthocyanins was determined by using cyanidin-3-O-glucoside as a standard, followed by investigating their antioxidant activity. The results showed that the content of total anthocyanins in mulberry extract was 314.30 μg/mg, which were mainly cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside, and cyanidin. The in vitro antioxidant activity experiments showed that the IC50 of the mulberry extract for scavenging DPPH·, ABTS+· and ·OH were 22.33 mg/mL, 12.90 mg/mL, and 1.74 mg/mL, respectively. Besides, 250 μg/mL mulberry extract had 0.744 reducing power. Therefore, mulberry extract has high anthocyanin content and good antioxidant activity, which provides a theoretical basis for developing and utilizing mulberry extract.
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