建立表面增强拉曼(surface-enhanced raman scattering,SERS)技术检测牛奶中三聚氰胺含量的方法。以金属钛板作为SERS衬底材料,采用50 nm银纳米颗粒作为基底,控制银溶胶与样品的体积比为1∶2,NaCl和NaOH溶液的浓度为4 mol/L,通过便携式拉曼光谱仪采集样品的SERS信号。在质量浓度0.2~10 mg/L的范围内,SERS强度随着牛奶中三聚氰胺浓度的增大而增强,线性相关系数R2=0.998,检测限为0.08 mg/L。使用银纳米基底对1 mg/L加标样品平行测定20次,三聚氰胺特征峰强度的相对标准偏差为4.34%。此法简单易行,重现性好、稳定性高,可实现对牛奶中三聚氰胺含量的快速检测,为食品中污染物的SERS检测提供了参考价值。
The surface-enhanced raman scattering (SERS) technique was used to detect melamine in milk. The metal titanium plate was used as a SERS substrate material, 50 nm silver nanoparticles were substrates, the ratio of the silver nanoparticles to the sample was 1∶2, and concentrations of both NaCl and NaOH were 4 mol/L. The SERS signal was acquired by a portable raman spectrometer. It was found that the intensity of SERS increased as the concentration of melamine increased from 0.2 mg/L to 10 mg/L (R2=0.998). Moreover, the detection limit was 0.08 mg/L and the relative standard deviation of the characteristic peak intensity of melamine was 4.34%. The established method was simple and easy to perform with good reproducibility and high stability. It can realize rapid detection of melamine in milk, which provides a reference for applying SERS in detecting pollutants in foods.
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